This data collection consists of water quality measurements, nutrient data and measured copper concentrations associated with experiments that derived site-specific guidline values (GVs) for tropical seawater microbiomes by integrating 16S rRNA gene amplicon sequencing with quantitative microbiome profiling and concentration-response modelling. Seawater was collected during high tide at three target sites adjacent to the Burdekin region of the Great Barrier Reef, representing varying disturbance histories: (1) Davies Reef, a high ecological value (HEV) coral reef reference site (S 18°49.826’ E 147°38.177’) situated 50 km offshore with low levels of human impacts and part of the Marine Protected Area; (2) Magnetic Island, a slightly to moderately disturbed (SD-MD) inshore coral/algal reef site (S 19°09.279’ E 146°52.108’) with some perturbations from shipping traffic, dredging, industry, and agricultural runoff; (3) Townsville Breakwater in close proximity to the Port of Townsville, a highly disturbed (HD) inshore site (S 19°14.629’ E 146°49.989’) with increased influence from shipping traffic, industry, and urban runoff. Seawater from each site was subsequently used in a 48-h copper exposure experiment. Water quality measurements (dissolved oxygen, pH and salinity) and water samples for nutrients (dissolved organic / inorganic carbon and nitrogen) were taken at 0-h and 48-h, following standard AIMS procedures. Representative water samples from the nominal experimental treatments (0, 0.1, 0.3, 1, 3, 10, 30, 60, 100, 300, 1000, 3000, 8000 μg Cu L-1) were sent to Envirolab for dissolved copper quantification. Data was entered into excel.

The dataset includes the accession numbers to the RNA sequences and qPCR data for pathogens and validation of some important RNA sequences. This dataset is associated with the following publication: Lu, J., I. Struewing, and N. Ashbolt. Meta-Transcriptomic Response to Copper Corrosion in Drinking Water Biofilms. Microorganisms. MDPI, Basel, SWITZERLAND, 13(7): 1528, (2025).

This dataset characterizes the acute toxicity of copper and zinc to white sturgeon (~30 days post hatch) and cladocerans (less thab 24 hours old) in waters with varying dissolved organic carbon (DOC) by conducting 96-hour white sturgeon and 48-hour cladoceran copper and zinc exposures in test waters with natural DOC (from pond water) at concentrations ranging from 0.5 to 5.5 mg/L.

Historical lead mining in Madison County, Missouri, USA has left a legacy of metals contamination. Laboratory studies were conducted to examine the effect of water hardness and age of crayfish on the toxicity of environment-based metals mixture. Additionally, the effects of chronic exposure to a metals mixture to egg development in adult crayfish were assessed. These data are comprised of measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead), major anions (e.g., chloride, sulfate), dissolved organic carbon, and major cations (e.g, sodium, calcium, magnesium) in water samples collected during laboratory-based diluter exposure studies.

Historical lead mining in Madison County, Missouri, USA has left a legacy of metals contamination. Laboratory studies were conducted to examine the effect of water hardness and age of crayfish on the toxicity of environment-based metals mixture. Additionally, the effects of chronic exposure to a metals mixture to egg development in adult crayfish were assessed. These data are comprised of measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead), major anions (e.g., chloride, sulfate), dissolved organic carbon, and major cations (e.g, sodium, calcium, magnesium) in water samples collected during laboratory-based diluter exposure studies.

Historical lead mining in Madison County, Missouri, USA has left a legacy of metals contamination. Laboratory studies were conducted to examine the effect of water hardness and age of crayfish on the toxicity of environment-based metals mixture. Additionally, the effects of chronic exposure to a metals mixture to egg development in adult crayfish were assessed. These data are comprised of measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead), major anions (e.g., chloride, sulfate), dissolved organic carbon, and major cations (e.g, sodium, calcium, magnesium) in water samples collected during laboratory-based diluter exposure studies. Measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead) in tissue samples (crayfish whole bodies, ovaries, leaves, feed) collected during laboratory-based diluter exposure studies are also provided.

Historical lead mining in Madison County, Missouri, USA has left a legacy of metals contamination. Laboratory studies were conducted to examine the effect of water hardness and age of crayfish on the toxicity of environment-based metals mixture. Additionally, the effects of chronic exposure to a metals mixture to egg development in adult crayfish were assessed. These data are comprised of measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead), major anions (e.g., chloride, sulfate), dissolved organic carbon, and major cations (e.g, sodium, calcium, magnesium) in water samples collected during laboratory-based diluter exposure studies. Measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead) in tissue samples (crayfish whole bodies, ovaries, leaves, feed) collected during laboratory-based diluter exposure studies are also provided.

This dataset consists of trace organic contaminant concentrations and bacteria counts in wastewater effluents, and observed responses in fish exposed to effluents. The study was conducted from April to September 2022 at a facility located in Hutchinson, Minnesota. Nine pairs of primary-treated, activated sludge secondary treated, and membrane bioreactor secondary treated wastewater were characterized for select trace organic contaminants (pharmaceuticals, pesticides and pesticide degradates, hormones, alkylphenols, and bisphenols) and bacterial (total coliform, Escherichia coli) counts. Two 21-day exposures were conducted on-site in which fathead minnows were exposed to activated sludge secondary treated effluent and membrane bioreactor secondary treated effluent. Effluent samples were collected once per month in April, August, and September, and once weekly during the on-site exposures.