This record provides an overview of the NESP Marine and Coastal Hub Research Plan 2023 project "Eastern Grey Nurse Shark population abundance and trend". For specific data outputs from this project, please see child records associated with this metadata. Australia’s eastern grey nurse shark population is listed as Critically Endangered under the Environment Protection and Biodiversity Conservation Act 1999. The Recovery Plan for the Grey Nurse Shark identifies research and actions needed to improve the shark’s population status and ensure its long-term conservation. This project refines the abundance and trend estimate for the eastern Australian population of grey nurse shark and examined the shark’s expansion range into Victorian waters (evidence suggests the shark may now range as far west as Wilsons Promontory). The project builds upon previous research which has estimated the eastern grey nurse shark adult population at ~2,000 individuals. Epigenetic techniques are used estimate age of juveniles (based on patterns of DNA methylation), and stereo video analysis is used to obtain precise length measurements for use against growth curves. Understanding the species' population structure is important to inform the assessment of current conservation arrangements such as spatial closures. Findings will provide a more precise estimate of the abundance of Australia’s eastern grey nurse shark population, and reduce uncertainty surrounding trends in abundance by building a stronger evidence base for decision-making around the species’ recovery and conservation. Outputs • Tissue samples and extracted products from juvenile grey nurse sharks [dataset] • Final project report [written]

This record provides an overview of the scope and research output of NESP Marine Biodiversity Hub Project A9 - "Grey Nurse Shark CK-MR Population Estimate – East Coast". For specific data outputs from this project, please see child records associated with this metadata. A review of the 2002 National Recovery Plan for Grey Nurse Shark (DEWHA 2009) concluded it was not possible to determine if the east coast population had shown any signs of recovery (DoE 2014); recommending a new recovery plan be developed for this species. A primary objective of the new recovery plan (DoE 2014) is to improve knowledge of GNS population status. This will require a robust estimate of population size and trend – something that has not been provided to date. This project will use genetic SNP data to inform close kin-mark recapture analysis to estimate population size and trend, and provide guidance on future monitoring strategies for the east coast population of grey nurse shark. Planned Outputs • Tools to refine and integrate CK-MR and species demographic data for population assessments of a key threatened species at a national scale (combining knowledge developed under this project combined with similar techniques being applied under NESP to euryhaline sharks and white sharks). • A national estimate of (census) population size and trend for the eastern Australian population of grey nurse shark will be developed to fulfil the highest priority actions of the National Recovery Plan. • Identify national strategies to guide future monitoring of grey nurse shark populations. • The project will provide peer-reviewed additions to the scientific literature that will add to the science-support for the development and implementation of policies to support the ecologically sustainable management of Australia’s marine environment.

This record provides an overview of the NESP Marine and Coastal Hub project "Updating knowledge of Australian white sharks". For specific data outputs from this project, please see child records associated with this metadata. The white shark is listed as vulnerable and migratory under Australia’s Environment Protection and Biodiversity Conservation Act 1999. The national White Shark Recovery Plan 2013 sets out research and management actions necessary to support the recovery of the white shark in Australian waters. Previous research funded by the National Environmental Science Program (NESP) provided updated estimates of white shark breeding population size and trend. However, the results were based on modest data sets and were limited by some critical knowledge gaps in relation to pupping and juvenile nursery areas, and uncertainty about how populations are connected between eastern and south-western Australia. Recent unpublished work has raised the prospect of a single Australian population. The White Shark Recovery Plan 2013 has identified a critical need for a quantitative assessment of population trends and evidence of any recovery of the white shark in Australian waters. This project will provide an update and reduce uncertainty regarding the status, trends, and population structure of white sharks in Australian waters. Specifically, it will focus efforts to identify critical habitats and biologically important areas for white sharks and improve the understanding of population status through advancing close-kin mark recapture research. Three project sub-components will involve: • Investigating the feasibility of filling knowledge gaps about juvenile and pupping areas and adult movements; • Investigating population structure to resolve mixing/connectivity questions; and • Updating population estimates based on significant new data. The project approach will comprise of: (1) A pilot study to assess the effectiveness of tagging adult females (>4.5 metres) and juveniles (>2 m) throughout the southern-western white shark range. Genetic samples will be gathered from around Australia and sought from South Africa and New Zealand to conduct a comprehensive update of white shark stock structure. (2) Using an expanded tissue sample set from New South Wales (~1000 samples) to update and refine estimates of adult population size and population trend for the eastern white shark population. Juvenile numbers will be estimated using data from the New South Wales shark management program. Additional samples from South Australia and Western Australia will be combined with previous samples in the southern-western population to refine estimates of population size. (3) Population estimates undertaken using close-kin mark-recapture, a technique that combines advanced genetics and statistical modelling to infer population demographics by identifying close-kin-pairs (parent-offspring or half-siblings) among a collection of sampled animals. Outputs • New genetic samples and sequencing data for white sharks [dataset] • Tracking data derived from 12 PAT tags [dataset] • Final technical report (including recommendations for systematic future research to assist in identifying additional critical habitat for the south-western white shark population) [written]

This record provides an overview of the scope and research output of NESP Marine Biodiversity Hub Project A3 - "A national assessment of population status of White Sharks". For specific data outputs from this project, please see child records associated with this metadata. White sharks are listed as Vulnerable under the EPBC Act and the subject of a national recovery plan, yet there is still no effective way to assess their population status and thus no way of determining the efficacy of conservation actions. Recent debate due to various human-shark interactions has highlighted the need for further information. This Project provides a national assessment of white shark population size and status in order to establish the efficacy of existing recovery actions and provide a scientifically sound and rational basis from which to develop policies that balance conservation objectives and public safety. Planed Outputs • Tools to refine and integrate CK-MR, electronic tagging distribution and species demographic data for population assessments of a key threatened species at a national scale (combining knowledge developed under this project combined with similar techniques being applied under NESP to euryhaline sharks and planned for grey nurse sharks). • National estimates of (census) population size and trend for white sharks in Australian waters (western and eastern populations respectively) are established that fulfil the highest priority actions of the National Recovery Plan. • New genetic and statistical tools trialled for the estimation of historical population trend from contemporary tissue samples for key species for which other methods of population assessment are unreliable or unavailable. • Provide information that identifies movement corridors, hotspots and contributes to management strategies for top-order marine predators • Estimate juvenile white shark survival and abundance for input into integrated national population assessment models in order to refine population estimates. • National-level information on habitat use, behaviour and spatial dynamics of white sharks at various scales used to provide the scientific underpinning for government decisions and policies as well as provide for more informed public debate. • Identify national strategies to monitor white shark populations. • The project will provide peer-reviewed additions to the scientific literature that will add to the science-support for the development and implementation of policies to support the ecologically sustainable management of Australia’s marine environment.

This record provides an overview of the scope and research output of NESP Marine Biodiversity Hub Project A11 - "Shark action plan". No datasets were generated by this project. Conservation of elasmobranch species (sharks and rays) is an increasing priority globally, including Australia, as evidence of overexploitation of some species becomes apparent. Common issues and threats among elasmobranch species may improve management if considered holistically. This project will produce a Shark Action Plan assessing requirements for improved management including a summary of current status across the taxa, guidelines for reducing impacts and improving management, and identification of key knowledge gaps impeding conservation and management. This Plan will help guide policy for Australian elasmobranchs developed by DoEE and fishery managers. On-ground conservation will be developed from recommendations in this plan. Outputs • Shark Action Plan relevant to management of Australian elasmobranchs including an assessment of current threats, prioritised conservation and management actions for at-risk species and guidance on future management and data needs [written] • Manuscripts for scientific journals outlining the results of project components (eg, Conservation Dependent species framework) [written]

This record provides an overview of the NESP Marine and Coastal Hub small scale scoping study: Marine and Coastal Threatened Species and Communities. There are numerous species and ecological communities in Australia’s marine and coastal environments that have formal threatened conservation status. Through a process of targeted reviews, expert elicitation and workshops with end-users, domain experts and others, this project will identify and co-design prospective research projects that best deliver information that will notably improve the status of key selected threatened species in Australia’s marine and coastal environment. Outputs will include summaries of workshops outcomes and a set of co-designed research proposals for submission in future Hub annual research plans. This project also provides the platform for MAC Hub contributions to the Resilient Landscapes Hub Cross-Hub Mission Project 7.0 on Threatened and Migratory Species and Ecological Communities. Planned Outputs • Final technical report recommendations for policy makers of key findings for priority research areas [written]

This record provides an overview of the NESP Marine and Coastal Hub project 'Towards assessing the values of reefs in the southern Gulf of Carpentaria 2024 - 2025'. For specific data outputs from this project, please see child records associated with this metadata. The Gulf of Carpentaria Marine Park was only recently declared in 2013. The zoning plan for this marine park, which permits trawling within a specific trawling zone is due for renewal by 2028. There is a strong need to collect, analyse, synthesise and make publicly available information on the values and conditions of key habitats within the Marine Park, such as recently identified patch reefs and coral reefs, to contribute to this upcoming review of the management plan for this marine park. The limited scientific information that is available on the values of this marine park is hard to access and much of it has not been analysed or written up. In addition, the Traditional Owners hold much valuable information about the ecological and cultural values that should inform future park management decisions. This project aims to gather, synthesise and report on existing scientific data and information about coral and patch reefs in the marine park. This information will be presented to the Traditional Owners and other relevant stakeholders at community meetings or workshops. Documenting cultural values of the reefs in this marine park is beyond the scope of the current project and would require considerable further engagement with Traditional Owners. However, it is anticipated that presentation of existing scientific and biophysical data at community meetings will start this process. We will produce visual (e.g. photos and videos) outputs that illustrate the values of these reefs, in order to better inform stakeholders involved in decisions about future park management. Planned Outputs • Final technical report with analysed data and a summary of recommendations for policy makers of key findings [written]

This record provides an overview of the scope and research output of NESP Marine Biodiversity Hub Project A1 - "Northern Australian hotspots for the recovery of threatened euryhaline species". For specific data outputs from this project, please see child records associated with this metadata. Euryhaline elasmobranchs represent over half of the EPBC-listed threatened sharks and rays, with northern Australia of national importance for this threatened species community. Critical information gaps remain, limiting the implementation of Recovery Plan objectives. This project will fill many data gaps through the application of acoustic telemetry, traditional and advanced molecular research (population genetics and close-kin mark-recapture), life history studies and Indigenous knowledge and education. End-users, primarily the Department of the Environment, state and territory fisheries and wildlife agencies, and Indigenous organisations, will be provided with information necessary to improve management and facilitate recovery of these threatened species, focusing on three themes: (1) Monitoring and understanding euryhaline species; (2) Indigenous partnerships for management of euryhaline species, and; (3) Knowledge for the reassessment of river shark status. Planned Outputs • Updated assessment of river shark status • Manuscripts on ecology and status relevant to the management of threatened euryhaline species • Manuscripts on optimal design of acoustic receiver arrays and statistical methods for estimating mortality • Threatened marine species education package for Indigenous communities • Media releases around key field and engagement activities • Data and information outputs of this project will include distribution, extent of occurrence and area of occupancy estimates for key marine species, Indigenous knowledge on key species distribution and occurrence, mortality and survivorship data on key species, the first data on river shark age determination (an essential component of understanding demography), molecular data on population structure and population connectivity of key species, and lower population size estimate for Glyphis garricki. Data will be housed on appropriate explorable databases and made fully available to DOE • Refining the identification of biologically important areas (BIAs) within the NT and WA for threatened euryhaline elasmobranchs (using published BIA protocols)

This record provides an overview of the NESP Marine and Coastal Hub project Indigenous Ranger-led monitoring of threatened sawfish in the southern Gulf of Carpentaria. For specific data outputs from this project, please see child records associated with this metadata. This project will partner scientists with First Nations Ranger groups to assess population abundance, distribution and by-catch survival for sawfish species in the southern Gulf of Carpentaria. This project will develop capability in First Nations Ranger Groups that will directly assist with developing baseline status and trends for sawfish species in Australia (including the largetooth sawfish Pristis pristis, which is a priority species: https://www.dcceew.gov.au/sites/default/files/documents/threatened-species-action-plan-2022-2032.pdf). Developing sampling and monitoring capability within First Nations Ranger groups in the southern Gulf of Carpentaria will increase the number of tissue samples for close kin mark recapture (CKMR) population estimates, potentially reducing the present reliance on the fishing industry to collect samples, and ultimately enable sawfish population trajectories to be measured. The CSIRO project team will facilitate capacity building of four Ranger teams within the CLCAC Normanton, Gangaliida and Garawa (Burketown), Wellesley Islands and Ngumari Waanyi (Gregory) to undertake sawfish surveys in the Southern Gulf. The training will build the skills of the Rangers to undertake sawfish surveys to collect tissue samples, distribution data and satellite tagging on CLCAC sea country. Tissue samples collected will contribute directly to the current NESP Sawfish project 3.11 “Multi-fishery collaboration to assess population abundances and post-release survival of threatened sawfish in northern Australia”, which is focused on working with the commercial fishing industry. These samples will be used to obtain baseline estimates of sawfish population status required to monitor population trends. Planned Outputs • Catch data from research surveys and/or indigenous ranger programs (Tabular data) • Tissue samples collected by research and or indigenous ranger programs • Final technical report with analysed data and a short summary of recommendations for policy makers of key findings [written]

This record provides an overview of the NESP Marine and Coastal Hub small-scale study - "Conservation of spotted handfish". For specific data outputs from this project, please see child records associated with this metadata. Spotted Handfish (Brachionichthys hirsutus) were once common across the bays and estuaries of South-eastern Tasmania. By 1996, however, populations had declined and the species was listed as Critically Endangered under Australia’s Environment Protection and Biodiversity Conservation Act 1999. The species is relatively short-lived, (5–10 years) and matures at more than two years’ old. This leaves a short window for reproduction, which relies on egg masses laid on seafloor structures such as stalked ascidians. If spawning fails, population declines may occur rapidly. With no planktonic life stage to aid dispersal, and low adult dispersal, outside recruitment to re-establish collapsed populations is unlikely. Analysis of 23 years of Spotted Handfish survey data (1997–2019) supported by previous NESP Marine Biodiversity Hub work (Project A10) helped develop a time-series of survey data, increasing biological understanding and contributing to effective management actions. This project recommenced surveys of multiple local populations, after a two-year gap, to ensure that the potential impacts of development of the Derwent estuary and surrounds handfish populations or their habitats can be detected. Included in the population surveying was identification of suitable locations to plant Artificial Spawning Habitats (ASH) where natural spawning structures have declined. This will continue to support the species' captive breeding program with industry and foster engagement with the indigenous and broader community through participation, talks, outreach, publications, and the National Handfish Recovery Team (NHRT). Outputs • A consolidated database of all available data on spotted handfish imagery, length frequency, and GPS regions to 2022 [time-series database] • Final Project Report, including a short summary of recommendations for policy makers of key findings [written]