Preliminary Metadata record for data expected from ASAC Project 1126 See the link below for public details on this project. ---- Public Summary from Project ---- Previous work on anti-freeze proteins (AFPs) in bacteria isolated from saline lakes in the Vestfold Hills, has shown that only around 10% of isolates possessed AFP activity. This suggests that the majority of bacteria may be using other mechanisms to avoid freezing or possibly are non-functional at sub-zero temperatures. We propose building on our previous work to ascertain if AFP occurrence is characteristic of particular taxonomic groups, or whether its evolution is random among different species. The fields in this dataset are: Lake Date Air Temperature Ice Thickness Sample Type Depth Height of ice core sample from ice/water interface Thickness of Ice core sample Salinity Water Temperature Nitrate Nitrite Ammonia Phosphate Bacteria Flagellates Chlorophyll DOC - Dissolved Organic Carbon COV of DOC - Coefficient of Variance

This metadata record will contain the results of bioassays conducted to characterise the response of Antarctic near-shore marine zooplankton to hydrocarbon contaminants in Special Antarctic Blend (SAB) diesel fuels commonly used in Antarctica. The results from one summer season (2010-11) are in this record. This was conducted under the AAS Project 3054: Ecological risks from oil products used in Antarctica: characterising hydrocarbon behaviour and assessing toxicity on sensitive early life stages of Antarctic marine invertebrates. Exposure solutions of fuel were experimentally mixed by slow stir of fuel and seawater in temperature control cabinets at -1 degree C to prepare a mixture of fuel hydrocarbons in filtered seawater (FSW) termed the Water Accommodated Fraction (WAF). WAF was produced by adding fuel to seawater in 5 L or 10 L Pyrex glass bottles using a ratio of 1:24 Fuel : FSW. This mixture was stirred at slow speed with minimal vortex for 18 h on a magnetic stirrer. The mixture was settled for 6 h before the water portion was drawn from beneath the fuel. Ecotoxicological bioassays were conducted at Davis Stations in the 2010/11 summer season using SAB WAF to prepare experimental treatments consisting of WAF dilution series. For each bioassay, treatments consisted of undiluted 100% WAF and dilutions of 10%, 17%, 25% and 50% of WAFs in FSW, to test the toxicity of water accommodated fractions of these three fuels on Antarctic both the zooplankton community and single copepod species. Bioassays were conducted in open vessels (glass jars or beakers) in temperature controlled cabinets. Mortality was observed at endpoints of 24 hrs, 48 hrs, 96 hrs, 7 days, 8 days, 9 days, 10 days, 11days, 12 days, 14 days, 15 days, and 16 days. New WAF solutions were prepared at the 7 day interval to replenish the experimental treatments. Deionised water was added to test solutions as required to maintain test solution volume and salinity. Water quality data was collected at each water change. Samples of test treatments for chemical analysis of hydrocarbon concentration were taken at each water change. Results of these analyses are not included as delayed progress with HC analyses impacted on quality of samples and these data were not used. This dataset consists of Excel spreadsheets. The file name code for zooplankton bioassays is; Project number_Season_Taxa_Test name Eg AAS_3054_10-11_zooplankton_m1 Project number : AAS_3054 Season : 2010/11 season Taxa: Zooplankton Community Test name: M1 =Multi-species test 1 Bioassay spreadsheets contain the results of bioassays for a species or the zooplankton community. Where replicate tests were conducted, each experiment is on a separate worksheet. The worksheet labelled "Test conditions" shows details of Test name, dates, animal collection details, laboratory holding conditions, details of water accommodated fractions (WAF), and bioassay conditions. The worksheet labelled "Counts" has a table for each of the replicates, arranged into a column for each treatment type. These tables show the number or dead individuals which were found and removed at each of the observation days. The worksheet labelled "Totals" has calculations of total number of individuals (of all species) which were found dead at each observation day in each replicate. It also gives the mean and standard deviation for each of the treatments. Further information on the zooplankton community structure in the 6 samples taken across the summer, based on the community in the toxicity tests and trials, is also included in the spreadsheet "AAS_3054_10-11_zooplankton_CommStructure". Sampling locations were near-shore from Davis Station, Vestfold Hills and from O'Gorman Rocks, southwest of Anchorage Island and northwest of Plough Island.

Metadata record for data from ASAC Project 1101 See the link below for public details on this project. ---- Public Summary from Project ---- Most of our knowledge of the Antarctic marine ecosystems comes from summer surveys. There are very few observations of this ecosystem in winter and there is a fundamental lack of knowledge of understanding of even basic questions such as 'what is there?' and 'what's it doing?'. The proposed visit to the sea ice zone in winter is a rare opportunity to conduct observations on phytoplankton, krill, birds, seals and whales, so that we can begin to understand the biological processes that go on in winter. Data for this project were intended to be collected on a 1998 winter voyage of the Aurora Australis, but a fire on board meant that the voyage had to return to port before work could be carried out. Data were then collected the following year during a 1999 winter voyage of the Aurora Australis (IDIOTS), which ran from July to September. Data attached to this metadata record, include zooplankton and CTD data collected from the Mertz Glacier region. The data have been compiled by Angela McGaffin, and can be found in the "processed" folder of the download file. Original datasets are also available in the "Original Datasets" folder.

The data describe all zooplankton species abundance values estimated at each rectangular midwater trawl (RMT 1+8) target and routine sampling site collected during the 2021 TEMPO voyage on R/V Investigator. During the TEMPO voyage, zooplankton were collected using the RMT 1+8 plankton net and sorted down to the lowest taxonomic level possible. The supporting data set, in CSV format, feature abundances estimated from RMT+1 samples only. The data file is named allzooplankton.csv. The fields (columns) in the file are: "Site" – sampling site ID for each routine (RXX) and target (TXX) trawl using RMT1+8. "Date" – date of each sample collected (ie. target or routine trawl) [dd/mm/yyyy]. "Latitude" – latitude of each trawl [degrees] (-dd.ddddd). "Longitude" – longitude of each trawl [degrees] (dd.ddddd). "Chla" – Integrated estimate of chlorophyll-a (mg m^-2) averaged at depths between 10 to 200 m, which aligns with the section of the water column where zooplankton were sampled. "Depth" – Bathymetric depth estimated at each sampling station. "MLD_03" – Mixed layer depth (m). Not used in final analysis. "MLD_05" – Mixed layer depth (m). Not used in final analysis. "MLD_N2max" – Mixed layer depth (m) where the N^2 maximum is found, i.e., the pycnocline. "CT_200" – Absolute temperature (°C) averaged at depths between surface and 200 m maximum net sampling depth. Values are derived from in situ CTD measurements at each sampling site. "SA_200" – Absolute salinity (g kg^-1) averaged at depths between surface and 200 m maximum net sampling depth. Values are derived from in situ CTD measurements at each sampling site. "IceMeltDays" – Number of days since sea ice melted calculated from daily passive microwave estimated percent sea ice concentration taken from the National Snow and Ice Data Centre. "m_to_Ice" – Distance (m) estimated from sampling site to edge of ice. "IceConc" – Actual ice concentration value (%). It is the percentage of an area covered in sea ice. The remaining columns are zooplankton species abundances (individuals per 1000 cubic metres) estimated from count data, volume of original sample, split of sample analysed under the microscope and calibration of the flowmeter: "R.gigas" – Rhincalanus gigas, copepod "C.propinquus" – Calanus propinquus, copepod "C.simillimus" – Calanus simillimus, copepod "C.acutus" – Calanoides acutus, copepod "Metridia sp." – Metridia species, copepod "M.gerlachei" – Metridia gerlachei, copepod "Paraeuchaeta sp." – Paraeuchaeta species, copepod "Pleuromamma sp." – Pleuromamma species, copepod "Oncaea" – Oncaea species, copepod "O.antarctica" – Oncaea antarctica, copepod "Neo tonsus" – Neocalanus tonsus, copepod "Neo gracilis" – Neocalanus gracilis, copepod "Stephos longipes" – Stephos longipes, copepod "Ctenocalanus sp." – Ctenocalanus species, copepod "Microcal pygmaeus" – Microcalanus pygmaeus, copepod "Oithona similis" – Oithona similis, copepod "Oithona frigida" – Oithona frigida, copepod "Heterhabdus australis" – Heterorhabdus australis, copepod "Heterhabdus sp." – Heterorhabdus species, copepod "Harpacticoid" – Harpacticoid species, copepod "Haloptilus sp." – Haloptilus species, copepod "Candacia sp." – Candacia species, copepod "Solecithricella sp." – Solecithricella species, copepod "Clausocalanus breviceps" – Clausocalanus breviceps, copepod "Clausocalanus laticeps" – Clausocalanus laticeps, copepod "Clausocalanus sp." – Clausocalanus species, copepod "Aetideops antarctica – Aetideopsis antarctica, copepod "Aetide minor" – Aetideopsis minro, copepod "Euchirella rostromanga" – Euchirella rostromanga, copepod "Heterostylites longicornis" – Heterostylites longicornis, copepod "Gaidius tenuispinus" – Gaidius tenuispinus, copepod "Gammaridean amphipod" – Gammaridean, amphipod "T.gaudichaudii" – Themisto gaudichaudii, amphipod "Parathemisto sp." – Parathemisto species, amphipod "Hyperia antarctica" – Hyperia antarctica, amphipod "Hyperia sp." – Hyperia species, amphipod "Hyperiella dilatata" –

The body surface, mouth, gills, internal organs and tissues of 368 teleost fish of 26 species from Prydz Bay, Heard Island, Macquarie Island, Davis Station and Casey Station in Antarctica were examined for parasites. At least eight species of Monogenea, seven species of Copepoda, and five or six species of Acanthocephala were recorded. Overall, the fauna of Monogenea and Copepoda of Antarctic fish is much poorer than that of lower latitudes, and there are fewer species of Gyrodactylidae relative to other Monogenea than at higher northern latitudes. Abundance and species richness of Acanthocephala are relatively high.