Between April 1, 2021, and March 31, 2024, the CFIA tested 858 samples of fresh and frozen seaweed salad for the presence of E. coli O157, Listeria monocytogenes, Salmonella, and generic E. coli. Of the samples tested, 98.1% were found to be satisfactory and required no further action. Samples that were found to be unsatisfactory underwent further actions, including further testing.

Between 2016 and 2021, 2398 samples of raw ground beef, veal and lamb were collected at retail stores across Canada. Samples were tested for STEC O157 and non-O157 STEC. STEC O157 and non-O157 STEC were found in 0.1% (3/2398) and 6.0% (145/2398) of the ground meat samples respectively. The prevalence of STEC O157 in Canadian retail raw ground meat is low, similar to ground beef produced from processing facilities. All STEC virulence gene profiles were assessed using criteria of the FAO/WHO risk categories and the majority of the STEC strains were classified as belonging to risk level 5 which is the lowest risk level. This study indicates that the current food safety control measures implemented for ground meats in Canada are effective at maintaining an acceptable level of possible contamination with STEC strains associated with severe clinical outcomes.

발표일 : 2024년 02월 08일, 시행일 : 2024년 08월 08일, 발표기관: 국가위생건강위원회, 국가시장감독관리총국 인 중국 GB 4789.40-2024 식품 미생물학 검사(크로노박터 검사) 번역문입니다. 원문은 표준은 식품 속 크로노박터(Cronobacter spp.) 검사 방법을 규정하고 있다.본 표준은 영유아 조제식품, 영유아 보조식품, 우유 및 유제품, 원료 속 크로노박터 검사에 적용됩니다. 해당 시스템에 접속하면 번역본에 대한 상세한 내용과 연혁, 원문 다운로드, 다운로드 가능합니다.본 법령 및 기준규격 콘텐츠는 번역 자료로 해당 내용의 최종 확인은 원문을 참고하시기 바랍니다.

,Foodborne pathogens are a significant cause of illness and infection with Shiga toxin-producing Escherichia coli (STEC) has the potential to produce life-threatening complications. The current methods to identify STEC in meat involve culture-based, molecular, and proteomic assays and take at least four days to complete. This time could be reduced by using long-read whole genome sequencing to identify foodborne pathogens. Therefore, the goal of this project was to evaluate using long-read sequencing to detect STEC in ground beef. The objectives of the project included: establishing optimal sequencing parameters, determining the limit of detection of all STEC virulence genes of interest in pure cultures and spiked ground beef, and evaluating selective sequencing to enhance STEC detection in ground beef. Sequencing libraries were run on Oxford Nanopore Technologies’ MinION sequencer. Optimal sequencing output was obtained using the default parameters in MinKNOW, except for setting the minimum read length to 1 kb. All genes of interest (eae, stx1, stx2, fliC, wzx, wzy, rrsC) were detected in DNA extracted from STEC pure cultures within 1 hour of sequencing, and 30X coverage was obtained within 2 hours. All virulence genes were confidently detected in STEC DNA quantities as low as 12.5 ng. In STEC inoculated ground beef, software-controlled selective sequencing improved virulence gene detection; however, several virulence genes were not detected due to high bovine DNA concentrations in the samples. Growth enrichment of inoculated meat samples in mTSB resulted in a 100-fold increase in virulence gene detection as compared to the unenriched samples. The results of this project suggest that further development of long-read sequencing protocols may result in a faster, less labor-intensive method to detect STEC in ground beef. The sequencing data from this project has been uploaded.,

중국 GB 4789.35-2023 식품 미생물학 검사(유산균 검사) 번역문입니다. 본 콘텐츠는 번역 자료로 해당 내용의 최종 확인은 원문을 참고하시기 바랍니다.

중국 GB 4789.26-2023 식품 미생물학 검사(상업적 무균 검사) 번역문입니다. 본 콘텐츠는 번역 자료로 해당 내용의 최종 확인은 원문을 참고하시기 바랍니다.