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Validation of a Novel Cell Culture Exposure System (CCES) for Studying the Toxicity of Volatile Chemicals at the Air-Liquid Interface
We developed a cell culture exposure system (CCES) to expose cells at the air-liquid interface (ALI) to volatile chemicals. We characterized the CCES by exposing indigo dye-impregnated filters inside each culture well to 125 ppb ozone (O3) for 1 h at flow rates of 5 and 25 mL/min/well; the reaction of O3 with an indigo dye produces a fluorescence product. We observed a 5-fold increase in fluorescence at 25 mL/min/well, suggesting higher flows were more effective. We then exposed primary human bronchial lung epithelial cells (HBECs) to 0.3 ppm acrolein for 2 h at 3, 5 and 25 mL/min/well and compared our results against well-established Human Studies Facility in vitro exposure chambers (HSF Chambers) at the U.S. EPA. We measured lactate dehydrogenase (LDH) release, and transcript changes of heme oxygenase-1 (HMOX1) and interleukin-8 (IL8) at 0, 1, and 24 h post-exposure. Comparing responses from the HSF Chamber to the CCES, differences were only observed at 1 h post-exposure for HMOX1. Here, the HSF Chamber produced a ~6-fold increase in HMOX1 while the CCES at 3 and 5 mL/min/well produced a ~1.7-fold increase. Operating the CCES at 25 mL/min/well produced a ~4.5-fold increase; slightly lower than the HSF Chamber. Our results suggest that higher flow rates in the CCES were more effective at delivering the gas to the cells, and this was further validated by our comparison against a well-established in vitro exposure system. Further testing is required to explore the sensitivity of the CCES with other chemicals. This dataset is associated with the following publication: Zavala-Mendez, J., A. Ledbetter, D.S. Morgan, L. Dailey, E. Puckett, S. McCullough, and M. Higuchi. A New Cell Culture Exposure System (CCES) for Studying the Toxicity of Volatile Chemicals at the Air-Liquid Interface. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 30(4): 169-177, (2018).
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Validation of a Novel Cell Culture Exposure System (CCES) for Studying the Toxicity of Volatile Chemicals at the Air-Liquid Interface
공공데이터포털
We developed a cell culture exposure system (CCES) to expose cells at the air-liquid interface (ALI) to volatile chemicals. We characterized the CCES by exposing indigo dye-impregnated filters inside each culture well to 125 ppb ozone (O3) for 1 h at flow rates of 5 and 25 mL/min/well; the reaction of O3 with an indigo dye produces a fluorescence product. We observed a 5-fold increase in fluorescence at 25 mL/min/well, suggesting higher flows were more effective. We then exposed primary human bronchial lung epithelial cells (HBECs) to 0.3 ppm acrolein for 2 h at 3, 5 and 25 mL/min/well and compared our results against well-established Human Studies Facility in vitro exposure chambers (HSF Chambers) at the U.S. EPA. We measured lactate dehydrogenase (LDH) release, and transcript changes of heme oxygenase-1 (HMOX1) and interleukin-8 (IL8) at 0, 1, and 24 h post-exposure. Comparing responses from the HSF Chamber to the CCES, differences were only observed at 1 h post-exposure for HMOX1. Here, the HSF Chamber produced a ~6-fold increase in HMOX1 while the CCES at 3 and 5 mL/min/well produced a ~1.7-fold increase. Operating the CCES at 25 mL/min/well produced a ~4.5-fold increase; slightly lower than the HSF Chamber. Our results suggest that higher flow rates in the CCES were more effective at delivering the gas to the cells, and this was further validated by our comparison against a well-established in vitro exposure system. Further testing is required to explore the sensitivity of the CCES with other chemicals. This dataset is associated with the following publication: Zavala-Mendez, J., A. Ledbetter, D.S. Morgan, L. Dailey, E. Puckett, S. McCullough, and M. Higuchi. A New Cell Culture Exposure System (CCES) for Studying the Toxicity of Volatile Chemicals at the Air-Liquid Interface. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 30(4): 169-177, (2018).
Benchmark Dose Modeling Approaches for Volatile Organic Chemicals using a Novel Air-Liquid Interface In Vitro Exposure System
공공데이터포털
Whole transcriptomics dose response data is collected and storedthrough public facing Gene Expression Omnibus database. Raw collected viability/cytotoxicity data for each chemical are collected and presented on separate spreadsheets. Portions of this dataset are inaccessible because: The transcriptomics full data file is too large to be uploaded alone onto ScienceHub, impeding ease of access. They can be accessed through the following means: Raw and processed transcriptomics data is available through Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) under accession GSE199794. Format: The full transcriptomics data set for all chemical conditions tested. This dataset is associated with the following publication: Speen, A., J. Murray, T. Krantz, D. Davies, P. Evansky, J. Harrill, L. Everett, J. Bundy, L. Dailey, W. Zander, E. Carlsten, M. Monsees, J. Hill, J. Zavala, and M. Higuchi. Benchmark Dose Modeling Approaches for Volatile Organic Chemicals using a Novel Air-Liquid Interface In Vitro Exposure System. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 188(1): 88-107, (2022).
Benchmark Dose Modeling Approaches for Volatile Organic Chemicals using a Novel Air-Liquid Interface In Vitro Exposure System
공공데이터포털
Whole transcriptomics dose response data is collected and storedthrough public facing Gene Expression Omnibus database. Raw collected viability/cytotoxicity data for each chemical are collected and presented on separate spreadsheets. Portions of this dataset are inaccessible because: The transcriptomics full data file is too large to be uploaded alone onto ScienceHub, impeding ease of access. They can be accessed through the following means: Raw and processed transcriptomics data is available through Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/) under accession GSE199794. Format: The full transcriptomics data set for all chemical conditions tested. This dataset is associated with the following publication: Speen, A., J. Murray, T. Krantz, D. Davies, P. Evansky, J. Harrill, L. Everett, J. Bundy, L. Dailey, W. Zander, E. Carlsten, M. Monsees, J. Hill, J. Zavala, and M. Higuchi. Benchmark Dose Modeling Approaches for Volatile Organic Chemicals using a Novel Air-Liquid Interface In Vitro Exposure System. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 188(1): 88-107, (2022).
Criteria pollutant impacts of volatile chemical products informed by near-field modeling
공공데이터포털
Dataset includes data appearing in main text figures and CMAQ code. This dataset is associated with the following publication: Qin, M., B. Murphy, K. Isaacs, B. McDonald, Q. Lu, S. McKeen, L. Koval, A. Robinson, C. Efstathiou, C. Allen, and H. Pye. Criteria pollutant impacts of volatile chemical products informed by near-field modeling. Nature Sustainability. Nature Publishing Group, New York, NY, USA, 1-57, (2020).
Criteria pollutant impacts of volatile chemical products informed by near-field modeling
공공데이터포털
Dataset includes data appearing in main text figures and CMAQ code. This dataset is associated with the following publication: Qin, M., B. Murphy, K. Isaacs, B. McDonald, Q. Lu, S. McKeen, L. Koval, A. Robinson, C. Efstathiou, C. Allen, and H. Pye. Criteria pollutant impacts of volatile chemical products informed by near-field modeling. Nature Sustainability. Nature Publishing Group, New York, NY, USA, 1-57, (2020).
Data for Volatile Chemical Product Enhancements to Criteria Pollutants in the United States
공공데이터포털
Data includes CMAQ code, CMAQ output, analysis scripts, CMAQ emission inputs, and VCPy emission framework code.
Data for Volatile Chemical Product Enhancements to Criteria Pollutants in the United States
공공데이터포털
Data includes CMAQ code, CMAQ output, analysis scripts, CMAQ emission inputs, and VCPy emission framework code.
Derivation of new Threshold of Toxicological Concern values for exposure via inhalation for environmentally-relevant chemicals
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An effort was made to derive new inhalation TTC values using the EPA’s Toxicity Values database, ToxValDB. A total of 4703 substances captured in ToxValDB were assigned into their respective TTC categories using the Kroes module within the Toxtree software tool and custom profilers developed in Nelms et al (2019) and Patlewicz et al (2018). For the substances assigned into the 3 Cramer classes, the 5th percentiles were calculated from the empirical cumulative distributions of No observed (adverse) effect level (concentration) values. The 5th percentiles were converted to their respective TTC values and compared with published values reported by Escher et al (2010) and Carthew et al (2009). The TTC values derived from ToxValDB were orders of magnitude more conservative, further Cramer classification was not found to be effective at discriminating potencies. This dataset is associated with the following publication: Nelms, M., and G. Patlewicz. Derivation of New Threshold of Toxicological Concern Values for Exposure via Inhalation for Environmentally-Relevant Chemicals. Frontiers in Toxicology. Frontiers, Lausanne, SWITZERLAND, 2: 580347, (2020).
Results of Section 4 Chemical Testing
공공데이터포털
The Toxic Substances Control Act (TSCA) requires that data be developed on the effect of chemical substances and mixtures on health and the environment. This data source collects the applicable test information on these chemicals submitted by external parties.
Results of Section 4 Chemical Testing
공공데이터포털
The Toxic Substances Control Act (TSCA) requires that data be developed on the effect of chemical substances and mixtures on health and the environment. This data source collects the applicable test information on these chemicals submitted by external parties.
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