These data represent water and bed sediment samples analyzed for a variety of organic compounds. The samples were collected in streams and rivers in the Chesapeake Bay watershed from 2006-2014. Water samples were collected from 61 sites and analyzed for hormones (SH2434 method; Tables 1A and 1B), pharmaceuticals (SH2080 method; Tables 2A and 2B), wastewater indicators (SH1433 method; Tables 3A and 3B), and antibiotics (LCAB method; Tables 4A and 4B). Select water samples were analyzed at the U.S. Geological Survey National Water Quality Laboratory for pesticides (SH2001 method and SH2003 method; Tables 5A/5B and 6A/6B, respectively), wastewater indicators (SH4433 method; Tables 7A and 7B), pharmaceuticals (SH8244 method; Tables 8A and 8B), and hormones (SH4434 method; Tables 9A and 9B). Bed sediment samples were collected from 56 sites and analyzed for wastewater compounds (SH5433 method; Tables 10A and 10B), hormones (SH6434 method; tables 11A and 11B), and organohalogen compounds (LC8093 method; Tables 12A and 12B). Water samples were analyzed for antibiotics at the U.S. Geological Survey Kansas Organic Geochemistry Laboratory. For each of the 12 laboratory methods there are two files, an analyte file (A; analytical method details) and data file (B; site information and analyte concentration if detected). The information can be downloaded in three formats: CSV (comma delimited; alldata-csv.zip), ASCII TXT (tab delimited; alldata-tab-delim-txt.zip), and XLSX (two Excel 2013 files; each dataset is a workbook tab; alldata.for.scibase.part-01.020317.xlsx [Tables 1-4, 10-12], alldata.for.scibase.part-02.020317.xlsx [Tables 5-9]). The Dataset XML file includes an complete list of Analyte Compounds, Compound Groups, CAS Numbers, and USGS NWIS Parameter codes found in each analyte file and associated data file. The analyte file for each method includes analyte compound names, compound group, CAS Number(s), USGS National Water Information System (NWIS) Parameter code, method detection limit, and reporting limit. Compound groups (in alphabetic order) include Androgen, Antibiotics Human and Veterinary, Antidepressant, Antihistamine, Antiviral, Beta-Blocker/Heart, Caffeine/Nicotine, Estrogen, Flame Retardant, Fungicide, Herbicide, Insecticide, Opiate, Persistent Organic Pollutant, Personal Care/Domestic Use (PCDU), Pesticide, Pharmaceutical, Plant/Animal Biochemical (PAB), Plasticizer, Polychlorinated Biphenyl, Polycyclic Aromatic Hydrocarbon, Progesterone, Progestin, and Stimulant/Abuse. The data file for each laboratory method includes State, Network, Sample Number, Station ID, Sample Date, Sample Time, Compound Group, Compound concentration. Network: CHEDC, data collected between 2012-2013 by the Chesapeake Endocrine Disruption project; PAREC, data available from Pennsylvania (other than Chesapeake Endocrine Disruption project samples)(various years listed in headnote); PA012, data available from Pennsylvania collected before 2013; C2007, data collected in Maryland, Virginia, and West Virginia between 2006-2007; CTEM, data collected at sites in West Virginia (various years listed in headnote). Station ID: When available, U.S. Geological Survey National Water Information (NWIS) site number. Sample time is standard time. Table head notes list sample media: filtered or unfiltered water, or bed sediment. Analyte Concentrations Units are listed in the table headnote (micrograms per kilogram OR nanograms per liter); nd denotes non-detect; dnq denotes detected, but not quantified; concentrations in italics denote replicate of previous sample. Site locations (including latitude/longitude) are provided in interactive map viewer, downloadable ESRI ArcGIS Shape file (Chesapeake_Bay_Watershed_water_and_sediment_sampling_sites_for_ScienceBase4.zip), and ArcGIS Mapping Service (REST Service).

This data release provides contaminant data from bed sediment collected from small streams between 2021 and 2024 in the Chesapeake Bay Watershed. These data are part of a larger study designed to determine how conservation practices are affecting local stream ecosystem health, which stressors are negatively impacting streams, and which landscape characteristics are associated with better or worse conditions in local streams. Samples were analyzed at RTI Laboratories Incorporated, Livonia, Michigan and The U.S. Geological Survey, Florence Bascom Geoscience Center, Wetland Ecosystem Ecology and Biogeochemistry Lab, Reston, Virginia. Exact site location information for these sites is not available as a result of privacy concerns.

Over 300 samples were collected in New York, Vermont, and Massachusetts from wastewater treatment facilities (20 locations), drinking water facilities (9 locations), streams (53 locations), lakes (2 locations), a bay (1 location), and a POCIS (Polar Organic Chemical Integrative Sampler) blank sample between April 2009 and October 2018. This data release provides chemistry data for over 400 analytes, including pharmaceuticals, hormones, personal care and domestic use compounds, pesticides and other compounds using 10 different laboratory analytical methods on water, sediment, wastewater sludge, and POCIS samples. Quality assurance data, such as equipment and field blank data and percent recovery for isotopic dilution standards, are included when available for a method.

Over 300 samples were collected in New York, Vermont, and Massachusetts from wastewater treatment facilities (20 locations), drinking water facilities (9 locations), streams (53 locations), lakes (2 locations), a bay (1 location), and a POCIS (Polar Organic Chemical Integrative Sampler) blank sample between April 2009 and October 2018. This data release provides chemistry data for over 400 analytes, including pharmaceuticals, hormones, personal care and domestic use compounds, pesticides and other compounds using 10 different laboratory analytical methods on water, sediment, wastewater sludge, and POCIS samples. Quality assurance data, such as equipment and field blank data and percent recovery for isotopic dilution standards, are included when available for a method.

Data from 10-day sediment toxicity tests of bed sediments from the Niagara River area of concern and tributaries, Niagara and Erie County, New York. Specifically, the data was used to compare the survival and growth of two macroinvertebrate species in sediments from study sites and laboratory controls. Results are from 10-day sediment exposures of two test species, Chironomus dilutus and Hyalella azteca. Bed sediments were collected from depositional areas using either a petite Ponar (0.03 square meter) dredge or a stainless steel sediment scoop. At each site, approximately five grabs or scoops were composited into a bucket, mixed, and a 4-liter (L) subsample was stored in a polyethylene container. Sediment toxicity testing were then conducted by a contract laboratory to quantify toxicity with the dipteran, Chironomus dilutus, and the amphipod, Hyalella azteca, during 10-day survival and growth bioassays following USEPA test methods 100.2 and 100.1, respectively. U.S. Environmental Protection Agency, 2000, Methods for measuring the toxicity and bioaccumulation of sediment associated contaminants with freshwater invertebrates. Second edition: U.S. Environmental Protection Agency, Office of Research and Development EPA 600/R-99/064.http://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=30003SBA.TXT. The data include the year, site ID, and six columns of results from 10-day sediment exposures of two test species, Chironomus dilutus and Hyalella azteca. The following endpoints were measured for each species following the 10-day exposure: percentage of organisms surviving (hereafter survival), average ash-free dry weight of the surviving organisms (hereafter growth), and average ash-free dry weight of the surviving organisms divided by the initial number of organisms (hereafter biomass). Companion to this report: George, S.D., Baldigo, B.P., and Duffy, B.T., 2016, Toxicity of bed sediments from the Niagara River Area of Concern and tributaries, New York, to Chironomus dilutus and Hyalella azteca, 2014–15: U.S. Geological Survey Data Series 1016, XX p., http://dx.doi.org/10.3133/ds1016.

Data from 10-day sediment toxicity tests of bed sediments from the Niagara River area of concern and tributaries, Niagara and Erie County, New York. Specifically, the data was used to compare the survival and growth of two macroinvertebrate species in sediments from study sites and laboratory controls. Results are from 10-day sediment exposures of two test species, Chironomus dilutus and Hyalella azteca. Bed sediments were collected from depositional areas using either a petite Ponar (0.03 square meter) dredge or a stainless steel sediment scoop. At each site, approximately five grabs or scoops were composited into a bucket, mixed, and a 4-liter (L) subsample was stored in a polyethylene container. Sediment toxicity testing were then conducted by a contract laboratory to quantify toxicity with the dipteran, Chironomus dilutus, and the amphipod, Hyalella azteca, during 10-day survival and growth bioassays following USEPA test methods 100.2 and 100.1, respectively. U.S. Environmental Protection Agency, 2000, Methods for measuring the toxicity and bioaccumulation of sediment associated contaminants with freshwater invertebrates. Second edition: U.S. Environmental Protection Agency, Office of Research and Development EPA 600/R-99/064.http://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=30003SBA.TXT. The data include the year, site ID, and six columns of results from 10-day sediment exposures of two test species, Chironomus dilutus and Hyalella azteca. The following endpoints were measured for each species following the 10-day exposure: percentage of organisms surviving (hereafter survival), average ash-free dry weight of the surviving organisms (hereafter growth), and average ash-free dry weight of the surviving organisms divided by the initial number of organisms (hereafter biomass). Companion to this report: George, S.D., Baldigo, B.P., and Duffy, B.T., 2016, Toxicity of bed sediments from the Niagara River Area of Concern and tributaries, New York, to Chironomus dilutus and Hyalella azteca, 2014–15: U.S. Geological Survey Data Series 1016, XX p., http://dx.doi.org/10.3133/ds1016.

Over 300 samples were collected in New York, Vermont, and Massachusetts from wastewater treatment facilities (20 locations), drinking water facilities (9 locations), streams (53 locations), lakes (2 locations), a bay (1 location), and a POCIS (Polar Organic Chemical Integrative Sampler) blank sample between April 2009 and October 2018. This data release provides chemistry data for over 400 analytes, including pharmaceuticals, hormones, personal care and domestic use compounds, pesticides and other compounds using 10 different laboratory analytical methods on water, sediment, wastewater sludge, and POCIS samples. Quality assurance data, such as equipment and field blank data and percent recovery for isotopic dilution standards, are included when available for a method. First posted November 5, 2019, ver. 1.0 Revised January 2023, ver. 1.1

Over 300 samples were collected in New York, Vermont, and Massachusetts from wastewater treatment facilities (20 locations), drinking water facilities (9 locations), streams (53 locations), lakes (2 locations), a bay (1 location), and a POCIS (Polar Organic Chemical Integrative Sampler) blank sample between April 2009 and October 2018. This data release provides chemistry data for over 400 analytes, including pharmaceuticals, hormones, personal care and domestic use compounds, pesticides and other compounds using 10 different laboratory analytical methods on water, sediment, wastewater sludge, and POCIS samples. Quality assurance data, such as equipment and field blank data and percent recovery for isotopic dilution standards, are included when available for a method. First posted November 5, 2019, ver. 1.0 Revised January 2023, ver. 1.1

These data represent surface water and bed sediment samples analyzed for a variety of organic compounds. The samples were collected in streams and rivers in the Chesapeake Bay watershed from 2012-2018. Sites were located in Maryland, Pennsylvania, and West Virginia. Surface water samples were collected from 25 sites and analyzed for estrogenicity and an extensive suite of organic contaminants including hormones, pharmaceuticals, wastewater indicators, pesticides, phytoestrogens, and mycotoxins. Bed sediment samples were collected from 20 sites and analyzed for a suite of organic contaminants including hormones, wastewater indicators, pesticides, and organohalogens. Bed sediment was also analyzed for total organic carbon. Data also include replicate and blank quality assurance samples. This U.S. Geological Survey (USGS) data release contains daily-mean streamflow and water-quality data in the Chesapeake Bay watershed ranging from 2012 to 2018 (beginning and end dates may vary).

These data represent surface water and bed sediment samples analyzed for a variety of organic compounds. The samples were collected in streams and rivers in the Chesapeake Bay watershed from 2012-2018. Sites were located in Maryland, Pennsylvania, and West Virginia. Surface water samples were collected from 25 sites and analyzed for estrogenicity and an extensive suite of organic contaminants including hormones, pharmaceuticals, wastewater indicators, pesticides, phytoestrogens, and mycotoxins. Bed sediment samples were collected from 20 sites and analyzed for a suite of organic contaminants including hormones, wastewater indicators, pesticides, and organohalogens. Bed sediment was also analyzed for total organic carbon. Data also include replicate and blank quality assurance samples. This U.S. Geological Survey (USGS) data release contains daily-mean streamflow and water-quality data in the Chesapeake Bay watershed ranging from 2012 to 2018 (beginning and end dates may vary).