This dataset is compromised of proteomic results from four species of stony coral (Orbicella faveolata, Orbicella franksi, Montastraea cavernosa, and Colpophyllia natans) collected in Dry Tortugas over the course of a year during the onset of stony coral tissue loss disease (SCTLD) (Sept 2021 - Aug 2022).

This digitized histological slide collection includes tissue biopsies from wild colonies of Orbicella franksii (OFRA) located at three sites throughout the Dry Tortugas and collected at four time points to capture pre-disease and endemic disease states (September 2021, February 2022, June 2022, and August 2022). Slides were stained with either hematoxylin and eosin stains to highlight tissue morphology or Movat’s pentachrome stain to differentiate connective tissues (mucin, collagen, elastin, myosin). All slides were scanned on a digital slide scanner to a maximum magnification of 40x. All images are in an .svs format. This dataset only includes the raw histological slide collection and associated metadata from biopsied coral colonies. Results from the analysis of the histological slides will be available from partner labs in separate associated datasets.

A proteome profiling approach was taken to characterize the cellular biology of massive corals of the Upper Florida Keys exposed to experimentally elevated temperatures. Specifically, Orbicella faveolata specimens from two inshore (Cheeca Rocks & The Rocks) and one offshore reef (Little Conch) were incubated at either 33°C for 5 days or 32°C days for 31 days (with controls maintained at 30°C), and proteins were extracted from a subset of 16 samples (representing multiple coral genotypes at each temperature x time), separated by liquid chromatography, and sequenced by mass spectrometry (MS). The associated, open-access manuscript (Mayfield et al. 2021) includes all methodological details needed to interpret the proteomic data. However, I have nevertheless reiterated the majority of these details in this document to aid those in navigating the NCEI data submission. Alongside this detailed methodological treatise, I have also uploaded 16 RAW files generated by the MS (Q Exactive™, Thermo-Fisher Scientific [TFS]), 16 MZML (open-access MS mass peaks) files, 16 MZID (open-access MS results) files, two sequence libraries needed to query the proteomic data (fasta files for the coral host & its dinoflagellate [family Symbiodiniaceae] endosymbionts), and a tab-delimited “online supplemental data file” (OSDF) that 1) describes which samples were analyzed; 2) defines MS jargon and explains how to interpret the MS output; and 3) presents all data in a distilled format that is more amenable to analysis by traditional statistical approaches. Please note that there are 32 MZID files, rather than 16, because each of the 16 MZML files was separately queried against each of the two aforementioned fasta sequence libraries.

This data release includes new sub annual and mean annual Sr/Ca records from two species of massive coral, Orbicella faveolata (coral B3) and Siderastrea siderea (coral CG2), from the Dry Tortugas National Park, FL (DTNP). We combine these new records with published Sr/Ca data from three additional S. siderea coral (DeLong et al., 2014) to generate a 278-year long multi-species stacked Sr/Ca-SST record from DRTO.

This data release includes new sub annual and mean annual Sr/Ca records from two species of massive coral, Orbicella faveolata (coral B3) and Siderastrea siderea (coral CG2), from the Dry Tortugas National Park, FL (DTNP). We combine these new records with published Sr/Ca data from three additional S. siderea coral (DeLong et al., 2014) to generate a 278-year long multi-species stacked Sr/Ca-SST record from DRTO.

This data release includes new, sub-annual Strontium/Calcium (Sr/Ca) and annual linear extension rates covering a period between 1980 and 2012 for five colonies of the massive coral, Orbicella faveolata (O. faveolata). All five coral colonies were collected live by U.S. Geological Survey (USGS) scientists from the Dry Tortugas National Park (DTNP), Florida (FL) in August 2008 and May 2012.