Dataset for journal article 'Evaluation of 147 Perfluoroalkyl Substances for Immunosuppressive and other Activities Through Phenotypic Screening of Human Primary Cells'. Per- and polyfluoroalkyl substances (PFAS) are a large class of chemicals in widespread use for diverse applications in commerce resulting in significant presence in the environment. Extensive studies of several of the highly produced members of the class have demonstrated potential for adverse health consequences to humans and the environment as well as highly pervasive and persistent exposures. Here we report testing results of 147 PFAS substances in a phenotypic screening platform of primary human cell co-culture systems, the BioMAP® Diversity PLUS panel, used to model complex tissue and disease biology of organs (vasculature, immune system, skin, lung) and general tissue biology. PFAS were tested at four concentrations ranging from approximately 0.06 to 60 micromolar in order to minimize influence from confounding effects of polypharmacology resulting from qualitatively different activities at higher concentrations. We also compared response profiles for all PFAS with an existing database of responses for the BioMAP assays to identify other potential mechanisms of activity for this diverse chemical family. This dataset is associated with the following publication: Houck, K., K. Friedman, M. Feshuk, G. Patlewicz, M. Smeltz, M. Clifton, B. Wetmore, S. Velichko, A. Berenyi, and E. Berg. Evaluation of 147 perfluoroalkyl substances for immunotoxic and other (patho)physiological activities through phenotypic screening of human primary cells. ALTEX. Society ALTEX Edition, Kuesnacht, SWITZERLAND, 40(2): 248-270, (2023).

Dosing information, body weights during exposure and immune system endpoints. This dataset is associated with the following publication: DeWitt, J., W. Williams , J. Creech, and R. Luebke. Suppression of antigen-specific antibody responses in mice exposed to perfluorooctanoic acid: Role of PPARalpha and T- and B-cell targeting. JOURNAL OF IMMUNOTOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 13(1): 38-45, (2016).

Dosing information, body weights during exposure and immune system endpoints. This dataset is associated with the following publication: DeWitt, J., W. Williams , J. Creech, and R. Luebke. Suppression of antigen-specific antibody responses in mice exposed to perfluorooctanoic acid: Role of PPARalpha and T- and B-cell targeting. JOURNAL OF IMMUNOTOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 13(1): 38-45, (2016).

Supplementary material for "Kreutz, A.; Clifton, M.S.; Henderson, W.M.; Smeltz, M.G.; Phillips, M.; Wambaugh, J.F.; Wetmore, B.A. Category-Based Toxicokinetic Evaluations of Data-Poor Per- and Polyfluoroalkyl Substances (PFAS) using Gas Chromatography Coupled with Mass Spectrometry. Toxics 2023, 11, 463. https://doi.org/10.3390/toxics11050463". This dataset is associated with the following publication: Kreutz, A., M. Clifton, W. Henderson, M. Smeltz, M. Phillips, J. Wambaugh, and B. Wetmore. Category-Based Toxicokinetic Evaluations of Data-Poor Per- and Polyfluoroalkyl Substances (PFAS) using Gas Chromatography Coupled with Mass Spectrometry. Toxics. MDPI, Basel, SWITZERLAND, 11(5): 463, (2023).

Supplementary material for "Kreutz, A.; Clifton, M.S.; Henderson, W.M.; Smeltz, M.G.; Phillips, M.; Wambaugh, J.F.; Wetmore, B.A. Category-Based Toxicokinetic Evaluations of Data-Poor Per- and Polyfluoroalkyl Substances (PFAS) using Gas Chromatography Coupled with Mass Spectrometry. Toxics 2023, 11, 463. https://doi.org/10.3390/toxics11050463". This dataset is associated with the following publication: Kreutz, A., M. Clifton, W. Henderson, M. Smeltz, M. Phillips, J. Wambaugh, and B. Wetmore. Category-Based Toxicokinetic Evaluations of Data-Poor Per- and Polyfluoroalkyl Substances (PFAS) using Gas Chromatography Coupled with Mass Spectrometry. Toxics. MDPI, Basel, SWITZERLAND, 11(5): 463, (2023).

A table of known interferences for per- and polyfluoroalkyl substances using mass spectrometry.

A table of known interferences for per- and polyfluoroalkyl substances using mass spectrometry.

Dataset of medium throughput (96 well plate) in vitro nuclear receptor activation assays for peroxisome proliferator activated receptor alpha (PPARa), PPAR gamma (PPARg), and estrogen receptor (ER) across a panel of per- and polyfluoroalkyl substances (PFAS), fatty acids, and pharmaceuticals. This dataset is associated with the following publication: Evans, N., J. Conley, M. Cardon, P. Hartig, E. Medlock Kakaley, and L. Gray. In vitro activity of a panel of per- and polyfluoroalkyl substances (PFAS), fatty acids, and pharmaceuticals in peroxisome proliferator-activated receptor (PPAR) alpha, PPAR gamma, and estrogen receptor. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 449(116136): 1, (2022).

Dataset of medium throughput (96 well plate) in vitro nuclear receptor activation assays for peroxisome proliferator activated receptor alpha (PPARa), PPAR gamma (PPARg), and estrogen receptor (ER) across a panel of per- and polyfluoroalkyl substances (PFAS), fatty acids, and pharmaceuticals. This dataset is associated with the following publication: Evans, N., J. Conley, M. Cardon, P. Hartig, E. Medlock Kakaley, and L. Gray. In vitro activity of a panel of per- and polyfluoroalkyl substances (PFAS), fatty acids, and pharmaceuticals in peroxisome proliferator-activated receptor (PPAR) alpha, PPAR gamma, and estrogen receptor. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 449(116136): 1, (2022).