To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 15 April - 7 May 2009, belt transect surveys of coral population and diseases quantitative assessments were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) Cruise HI0903 in the Marianas Archipelago at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, there were 21 surveys in total conducted at REA sites around Pagan Island in the Marianas Archipelago. At the specific REA sites, coral biologists along with fish biologists, algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (~300 m2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of fish, corals, macro-invertebrates, and algae in shallow-water (< 35 m) habitats. As a part of REA surveys, the coral belt surveys were focused on quantifying the diversity, abundance, density, and size-class distribution of the anthozoan and hydrozoan corals as well as the condition and health state of the coral reef populations. The surveys were conducted along two consecutively-placed, 25m transect lines. The belt width was dictated at the beginning of each dive by subjective perceived colony density: 1-m width was used in high density areas, while 2-m width was used in low density areas. Within each 25m transect, five 2.5-meter segments were surveyed (beginning at points: 0, 5, 10, 15, and 20 meters), whereby in each segment, all coral colonies whose center fell within 0.5m of either side of the transect line were identified to the lowest taxonomic level possible (genus or species) and two planar size metrics were collected: maximum diameter and diameter perpendicular to the maximum diameter. In addition, the extent of mortality, both recent and old, was estimated for each colony. Observers paid special attention to identifying as best as possible the extent of the former live colony. When a coral colony exhibited signs of disease or compromised health, additional information was recorded including type of affliction (bleaching, skeletal growth anomaly, white syndrome, tissue loss other than white syndrome, trematodiasis, necrosis, other, pigmentation responses, algal overgrowth, and predation), severity of the affliction (mild, moderate, marked, severe, acute), as well as photographic documentation and sometimes tissue samples. Tissue samples were catalogued and fixed in buffered zinc-formalin solution for further histopathological analyses. Raw survey data included species presence and relative abundance, colony counts per taxon, size (width and length), mortality, predation, and health status. A surveyed area was ranging from 10m2 to 25m2 per site.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, reef fish assessment surveys were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) Cruise HA1101 in the Marianas region by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, 29 REA sites were surveyed at Pagan in the Marianas region. At each REA site, fish biologists entered the water and conducted a fine-scale (~700 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species diversity, size distribution, and abundance of fish in shallow-water hard-bottom (less than 30 m) habitats. Reef fish assessment surveys were focused on cataloging the diversity (species richness), abundance (numeric density) and biomass (fish mass per unit area) of diurnally active reef fish assemblages. The stationary point count (SPC) method was used to quantify reef fish species. Two divers lay out a 30 m transect line, and position themselves at the 7.5 and 22.5 meter marks. The SPC biologist then records estimated size and abundance of all fish within a visually estimated 15-m diameter cylinder centered on the stationary diver (7.5-m radius, total area ~ 177m^2 per cylinder). The diver first spends 5 minutes identifying all fish species in the cylindrical area, then proceeds to count and estimate size (total length) for each in a series of "instantaneous" point counts or sweeps of the cylinder. Fish were identified at the species level, wherever possible. All reef-associated fish, including those in the water column, were surveyed. The survey time for each stationary point count survey was approximately 20 min and generally four stationary point count surveys (two per diver) were conducted at each fish REA site. After completing REA surveys, divers noted the presence, at the survey site, of any unusual fish species not counted during SPC counts, in order to facilitate species lists per location.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, belt transect surveys of coral population and diseases quantitative assessments were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) cruise HA1101 in Marianas at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, there were 6 surveys in total conducted at REA sites around Tinian. At the specific REA sites, coral biologists along with algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (approximately 300 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of corals, macro-invertebrates, and algae in shallow-water (less than 35 m) habitats. As a part of REA surveys, the coral belt surveys were focused on quantifying the diversity, abundance, density, and size-class distribution of the anthozoan and hydrozoan corals as well as the condition and health state of the coral reef populations. The surveys were conducted along two consecutively-placed, 25 m transect lines. The belt width was 1 m wide, 0.5 m on each side of the transect line. Within each 25 m transect, five 2.5 m segments were surveyed (beginning at points: 0, 5, 10, 15, and 20 meters), whereby in each segment, all coral colonies whose center fell within 0.5 m of either side of the transect line were identified to the lowest taxonomic level possible (genus or species) and two planar size metrics were collected: maximum diameter and diameter perpendicular to the maximum diameter. In addition, the extent of mortality, both recent and old, was estimated for each colony. Observers paid special attention to identifying as best as possible the extent of the former live colony. When a coral colony exhibited signs of disease or compromised health, additional information was recorded including type of affliction (bleaching, skeletal growth anomaly, white syndrome, tissue loss other than white syndrome, trematodiasis, necrosis, other, pigmentation responses, algal overgrowth, and predation), severity of the affliction (mild, moderate, marked, severe, acute), as well as photographic documentation and occasional tissue samples. Tissue samples were cataloged and fixed in buffered zinc-formalin solution for further histopathological analyses. Raw survey data included species presence and relative abundance, colony counts per taxon, size (width and length), mortality, predation, and health status. A surveyed area was ranging from 10 m^2 to 25 m^2 per site.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, belt transect surveys of coral population and diseases quantitative assessments were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) cruise HA1101 in Marianas at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, there were 3 surveys in total conducted at REA sites around Sarigan. At the specific REA sites, coral biologists along with algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (approximately 300 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of corals, macro-invertebrates, and algae in shallow-water (less than 35 m) habitats. As a part of REA surveys, the coral belt surveys were focused on quantifying the diversity, abundance, density, and size-class distribution of the anthozoan and hydrozoan corals as well as the condition and health state of the coral reef populations. The surveys were conducted along two consecutively-placed, 25 m transect lines. The belt width was 1 m wide, 0.5 m on each side of the transect line. Within each 25 m transect, five 2.5 m segments were surveyed (beginning at points: 0, 5, 10, 15, and 20 meters), whereby in each segment, all coral colonies whose center fell within 0.5 m of either side of the transect line were identified to the lowest taxonomic level possible (genus or species) and two planar size metrics were collected: maximum diameter and diameter perpendicular to the maximum diameter. In addition, the extent of mortality, both recent and old, was estimated for each colony. Observers paid special attention to identifying as best as possible the extent of the former live colony. When a coral colony exhibited signs of disease or compromised health, additional information was recorded including type of affliction (bleaching, skeletal growth anomaly, white syndrome, tissue loss other than white syndrome, trematodiasis, necrosis, other, pigmentation responses, algal overgrowth, and predation), severity of the affliction (mild, moderate, marked, severe, acute), as well as photographic documentation and occasional tissue samples. Tissue samples were cataloged and fixed in buffered zinc-formalin solution for further histopathological analyses. Raw survey data included species presence and relative abundance, colony counts per taxon, size (width and length), mortality, predation, and health status. A surveyed area was ranging from 10 m^2 to 25 m^2 per site.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, belt transect surveys of coral population and diseases quantitative assessments were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) cruise HA1101 in Marianas at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, there were 9 surveys in total conducted at REA sites around Saipan. At the specific REA sites, coral biologists along with algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (approximately 300 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of corals, macro-invertebrates, and algae in shallow-water (less than 35 m) habitats. As a part of REA surveys, the coral belt surveys were focused on quantifying the diversity, abundance, density, and size-class distribution of the anthozoan and hydrozoan corals as well as the condition and health state of the coral reef populations. The surveys were conducted along two consecutively-placed, 25 m transect lines. The belt width was 1 m wide, 0.5 m on each side of the transect line. Within each 25 m transect, five 2.5 m segments were surveyed (beginning at points: 0, 5, 10, 15, and 20 meters), whereby in each segment, all coral colonies whose center fell within 0.5 m of either side of the transect line were identified to the lowest taxonomic level possible (genus or species) and two planar size metrics were collected: maximum diameter and diameter perpendicular to the maximum diameter. In addition, the extent of mortality, both recent and old, was estimated for each colony. Observers paid special attention to identifying as best as possible the extent of the former live colony. When a coral colony exhibited signs of disease or compromised health, additional information was recorded including type of affliction (bleaching, skeletal growth anomaly, white syndrome, tissue loss other than white syndrome, trematodiasis, necrosis, other, pigmentation responses, algal overgrowth, and predation), severity of the affliction (mild, moderate, marked, severe, acute), as well as photographic documentation and occasional tissue samples. Tissue samples were cataloged and fixed in buffered zinc-formalin solution for further histopathological analyses. Raw survey data included species presence and relative abundance, colony counts per taxon, size (width and length), mortality, predation, and health status. A surveyed area was ranging from 10 m^2 to 25 m^2 per site.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, belt transect surveys of coral population and diseases quantitative assessments were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) cruise HA1101 in Marianas at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, there were 13 surveys in total conducted at REA sites around Guam. At the specific REA sites, coral biologists along with algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (approximately 300 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of corals, macro-invertebrates, and algae in shallow-water (less than 35 m) habitats. As a part of REA surveys, the coral belt surveys were focused on quantifying the diversity, abundance, density, and size-class distribution of the anthozoan and hydrozoan corals as well as the condition and health state of the coral reef populations. The surveys were conducted along two consecutively-placed, 25 m transect lines. The belt width was 1 m wide, 0.5 m on each side of the transect line. Within each 25 m transect, five 2.5 m segments were surveyed (beginning at points: 0, 5, 10, 15, and 20 meters), whereby in each segment, all coral colonies whose center fell within 0.5 m of either side of the transect line were identified to the lowest taxonomic level possible (genus or species) and two planar size metrics were collected: maximum diameter and diameter perpendicular to the maximum diameter. In addition, the extent of mortality, both recent and old, was estimated for each colony. Observers paid special attention to identifying as best as possible the extent of the former live colony. When a coral colony exhibited signs of disease or compromised health, additional information was recorded including type of affliction (bleaching, skeletal growth anomaly, white syndrome, tissue loss other than white syndrome, trematodiasis, necrosis, other, pigmentation responses, algal overgrowth, and predation), severity of the affliction (mild, moderate, marked, severe, acute), as well as photographic documentation and occasional tissue samples. Tissue samples were cataloged and fixed in buffered zinc-formalin solution for further histopathological analyses. Raw survey data included species presence and relative abundance, colony counts per taxon, size (width and length), mortality, predation, and health status. A surveyed area was ranging from 10 m^2 to 25 m^2 per site.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, belt transect surveys of coral population and diseases quantitative assessments were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) cruise HA1101 in Marianas at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, there were 6 surveys in total conducted at REA sites around Agrihan. At the specific REA sites, coral biologists along with algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (approximately 300 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of corals, macro-invertebrates, and algae in shallow-water (less than 35 m) habitats. As a part of REA surveys, the coral belt surveys were focused on quantifying the diversity, abundance, density, and size-class distribution of the anthozoan and hydrozoan corals as well as the condition and health state of the coral reef populations. The surveys were conducted along two consecutively-placed, 25 m transect lines. The belt width was 1 m wide, 0.5 m on each side of the transect line. Within each 25 m transect, five 2.5 m segments were surveyed (beginning at points: 0, 5, 10, 15, and 20 meters), whereby in each segment, all coral colonies whose center fell within 0.5 m of either side of the transect line were identified to the lowest taxonomic level possible (genus or species) and two planar size metrics were collected: maximum diameter and diameter perpendicular to the maximum diameter. In addition, the extent of mortality, both recent and old, was estimated for each colony. Observers paid special attention to identifying as best as possible the extent of the former live colony. When a coral colony exhibited signs of disease or compromised health, additional information was recorded including type of affliction (bleaching, skeletal growth anomaly, white syndrome, tissue loss other than white syndrome, trematodiasis, necrosis, other, pigmentation responses, algal overgrowth, and predation), severity of the affliction (mild, moderate, marked, severe, acute), as well as photographic documentation and occasional tissue samples. Tissue samples were cataloged and fixed in buffered zinc-formalin solution for further histopathological analyses. Raw survey data included species presence and relative abundance, colony counts per taxon, size (width and length), mortality, predation, and health status. A surveyed area was ranging from 10 m^2 to 25 m^2 per site.

Coral health and disease assessments were conducted along 2 consecutively placed 25-m transects, as part of Rapid Ecological Assessments conducted at 9 sites at Pagan Island in the Marianas Archipelago during 25 May - 09 June 2007, aboard the NOAA Hi'ialakai Reef Assessment and Monitoring Program (RAMP) Cruise HI0703. Within an area of 1-3m on each side of both transect lines, each colony was inspected and every diseased/afflicted coral enumerated and identified to the lowest taxonomic level. For each affected coral the following information was recorded: colony size, type of affliction, area affected, percent live/dead, and severity of the affliction (mild = 1-10%, moderate = 11-25%, marked = 26-50%, severe = 51-75%, acute = 76-100%). Coral afflictions were classified into one of six general categories (following Willis et al., 2004) including: bleaching, tissue loss, black band disease, skeletal growth anomaly, predation, and other lesions. This latter category included algal overgrowth, as well as unidentified syndromes causing deterioration of scleractinian corals. Tissue samples of selected cases were procured for histopathological analyses. These data provide the basis for computing quantitative estimates of disease incidence and prevalence. Samples and photographs provide aid in further disease characterization and description. Histological tissue samples are traditionally fixed in a formaldehyde solution prior to preparation for processing and analysis.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, belt transect surveys of coral population and diseases quantitative assessments were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) cruise HA1101 in Marianas at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, there were 9 surveys in total conducted at REA sites around Maug. At the specific REA sites, coral biologists along with algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (approximately 300 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of corals, macro-invertebrates, and algae in shallow-water (less than 35 m) habitats. As a part of REA surveys, the coral belt surveys were focused on quantifying the diversity, abundance, density, and size-class distribution of the anthozoan and hydrozoan corals as well as the condition and health state of the coral reef populations. The surveys were conducted along two consecutively-placed, 25 m transect lines. The belt width was 1 m wide, 0.5 m on each side of the transect line. Within each 25 m transect, five 2.5 m segments were surveyed (beginning at points: 0, 5, 10, 15, and 20 meters), whereby in each segment, all coral colonies whose center fell within 0.5 m of either side of the transect line were identified to the lowest taxonomic level possible (genus or species) and two planar size metrics were collected: maximum diameter and diameter perpendicular to the maximum diameter. In addition, the extent of mortality, both recent and old, was estimated for each colony. Observers paid special attention to identifying as best as possible the extent of the former live colony. When a coral colony exhibited signs of disease or compromised health, additional information was recorded including type of affliction (bleaching, skeletal growth anomaly, white syndrome, tissue loss other than white syndrome, trematodiasis, necrosis, other, pigmentation responses, algal overgrowth, and predation), severity of the affliction (mild, moderate, marked, severe, acute), as well as photographic documentation and occasional tissue samples. Tissue samples were cataloged and fixed in buffered zinc-formalin solution for further histopathological analyses. Raw survey data included species presence and relative abundance, colony counts per taxon, size (width and length), mortality, predation, and health status. A surveyed area was ranging from 10 m^2 to 25 m^2 per site.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 15 April - 7 May 2009, reef fish assessment surveys were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) Cruise HI0903 in the Marianas Archipelago at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, 21 REA sites were surveyed at Pagan Island in the Marianas Archipelago. At the specific REA sites, fish biologists along with coral biologists, algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (~300 m2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of fish, corals, macro-invertebrates, and algae in shallow-water (< 35 m) habitats. Reef fish assessment surveys were focused on cataloging the diversity (species richness), abundance (numeric density [# fish 100 m-2] and biomass density [kg 100 m-2]) of diurnally active reef fish assemblages. Three complementary noninvasive underwater survey methods were used, including belt-transect, stationary point count, and roving-diver surveys. For all methods, fish were identified at the species level, when possible, and assigned to a size bin ranging from 1 to 200 cm based on a visual estimate of total fish length. Belt-transect (BLT) surveys were used to quantify the entire diurnal fish community (all size classes). In belt-transect surveys, two fish biologists swam side-by-side along three consecutively-placed, 25m transect lines. The BLT team swam each transect two times. During the initial swim-out, each fish diver recorded all fish larger than 20 cm observed within a 4-m wide belt perpendicular to their respective side of the transect (200 m2 area per line, 100 m2 per diver). On the return swim, each fish diver recorded all fish less than 20 cm observed within a 2-m wide belt (100 m2 area per line, 50 m2 per diver). The survey of large fish took approximately 5 min to complete while the survey of smaller fish took about 10 min to complete. All reef-associated fish, including those in the water column (including planktivores), were counted. Any coastal pelagic species (e.g., clupeids [sardines], belonids [beakfish], antherinids [silversides]) seen near the surface were not recorded. The stationary point count (SPC) method were used to quantify larger, more mobile reef fish species that can be missed on belt-transect surveys. In stationary point count survey, a fish biologist swam approximately 15 m away from a transect line concurrently being surveyed by the other two BLT fish biologists. The SPC biologist then recorded all fish greater than 25 cm in length that passed within a visually estimated 20-m diameter cylinder centered on the diver's fixed position (10-m radius, total area = 314 m2). The survey time for each stationary point count survey was 5 min and a total of four stationary point count surveys were conducted at each REA site. Roving-diver surveys were followed belt-transect and stationary point count surveys. As diver bottom time permitted, the fish assessment team conducted random swim surveys throughout the REA site area, recording, to the species level or the lowest recognizable taxon, the presence of reef fish not encountered during previous methods.