This dataset contains boundary polygons from 59 wetlands, that were monitored as part of the U.S. Geological Survey (USGS) Amphibian Research and Monitoring Initiative (ARMI) from Saint Marks National Wildlife Refuge (SMNWR). These sites covered a range of sizes, shapes, topographic and vegetative characteristics that were deemed to be representative of wetlands within the study area. Ground-based surveys of wetland perimeters were made using a Trimble R8 Real Time Kinematic Global Positioning System (RTK-GPS). Surveys generally followed the lowest extent of saw palmetto (Serenoa repens), which is a reliable indicator of the upper limits (elevation) of sustained inundation. In instances where palmetto was absent, margins were identified based on the lower limits of gallberry (Ilex sp.) and/or the upper extent of titi (Cyrilla/Cliftonia). These species occupied similar hydrologic and elevation zones as saw palmetto and, therefore, considered to also be reliable indicators of a wetland’s edge. GPS points were collected along this vegetative margin to represent the size and general shape of each wetland. In cases where dense vegetation prevented surveying the complete margin, we used elevation contours to connect distant points when digitizing perimeters. For a subset of wetlands that were not surveyed in the field on screen digitizing was used to construct the boundaries. When using this latter approach multiple layers were used to help identify the boundary including the national wetlands inventory, aerial imagery, and a lidar derived digital elevation model. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.