Genetic linkage map and comparative genome analysis for the estuarine Atlantic killifish (Fundulus heteroclitus)
공공데이터포털
Genetic linkage maps are valuable tools in evolutionary biology; however, their availability for wild populations is extremely limited. Fundulus heteroclitus (Atlantic killifish) is a non-migratory estuarine fish that exhibits high allelic and phenotypic diversity partitioned among subpopulations that reside in disparate environmental conditions. An ideal candidate model organism for studying gene-environment interactions, the molecular toolbox for F. heteroclitus is limited. We identified hundreds of novel microsatellites which, when combined with existing microsatellites and single nucleotide polymorphisms (SNPs), were used to construct the first genetic linkage map for this species. By integrating independent linkage maps from three genetic crosses, we developed a consensus map containing 24 linkage groups, consistent with the number of chromosomes reported for this species. These linkage groups span 2300 centimorgans (cM) of recombinant genomic space, intermediate in size relative to the current linkage maps for the teleosts, medaka and zebrafish. Comparisons between fish genomes support a high degree of synteny between the consensus F. heteroclitus linkage map and the medaka and (to a lesser extent) zebrafish physical genome assemblies. This dataset is associated with the following publication: Waits , E., J. Martinson , B. Rinner, S. Morris, D. Proestou, D. Champlin , and D. Nacci. Genetic linkage map and comparative genome analysis for the estuarine Atlantic killifish (Fundulus heteroclitus). Open Journal of Genetics. Scientific Research Publishing, Inc., Irvine, CA, USA, 6: 28-38, (2016).
Population genetics of the starfish, Linckia laevigata, in the Indian Ocean and the western Pacific Ocean
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A study of the population genetics of the starfish Linckia laevigata throughout the Indian and Pacific Oceans. Seven allozyme loci (GPI, ENO, HK, LP, LT-1, LT-2, SOD) were examined. Variations in gene frequencies of allozymes and common proteins, as well as mitochondrial D-loop DNA (the Restriction Fragment Length Polymorphism - RFLP) were used to estimate connectivity and dispersal between populations in the region. Genetic differentiation of populations within and among oceans.To determine whether colour morphs were genetically differentiated. 1020 individuals were sampled from 2 sites from each of 10 collection locations (Escape, Evening, Davies, Grub, Lodestone, Holbourne, Tern, Sanctuary, 22112, Sykes) between July 1991 and March 1992.608 individuals were added to the previous set (analyses included all samples). These were collected from 18 localities: Australia (Western Australia - Mermaid, Clerke, Imperieuse, Scott and Serangapatum Reefs - Torres Strait, Burke Reef); Fiji (Dravuni, Suva); Guam (Asan, Luminao); Japan (Sesoko, Okinawa); New Caledonia; Philippines (Bolinao, Dumaguete); Solomon Islands (Gizo, Guadacanal).92 individuals were collected from Thailand (Kuta Beach, Phuket Marine Station, Waeo Island) and South Africa (7 reefs in the St. Lucia and Maputuland Marine Reserves); analyses included previously collected data. These samples were of the orange colour morph and were compared the previous data in which the royal blue morph is predominant.
Spectacled Eider (Somateria fischeri) Microsatellite and Mitochondrial DNA Data, 2014-2018, Alaska and Russia
공공데이터포털
This data set describes nuclear microsatellite genotypes derived from ten autosomal loci (Aph8, Aph16, Cmo7, Cmo9, Hhi5, Sfi10, Smo4, Smo6, Smo8, Smo12) and nucleotide sequence data derived from one mitochondrial DNA locus (control region). A total of 262 Spectacled Eiders were examined for this study. Samples were collected at Indigirka and Chaun River Deltas, Russia, and Yukon-Kuskokwim Delta, Utqiagvik, Colville River Delta, Prudhoe Bay, Alaska. Samples used in the study originated from blood or feather samples collected in the field from live trapped birds or from tissue taken from dead birds.
Spectacled Eider (Somateria fischeri) Microsatellite and Mitochondrial DNA Data, 2014-2018, Alaska and Russia
공공데이터포털
This data set describes nuclear microsatellite genotypes derived from ten autosomal loci (Aph8, Aph16, Cmo7, Cmo9, Hhi5, Sfi10, Smo4, Smo6, Smo8, Smo12) and nucleotide sequence data derived from one mitochondrial DNA locus (control region). A total of 262 Spectacled Eiders were examined for this study. Samples were collected at Indigirka and Chaun River Deltas, Russia, and Yukon-Kuskokwim Delta, Utqiagvik, Colville River Delta, Prudhoe Bay, Alaska. Samples used in the study originated from blood or feather samples collected in the field from live trapped birds or from tissue taken from dead birds.
Molecular and morphological data to resolve species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli
공공데이터포털
We generated multilocus DNA sequence data and traditional morphometric measurements to evaluate species boundaries in Fusconaia mitchelli. We sequenced three loci: the protein-coding mitochondrial DNA genes cytochrome oxidase subunit 1 and NADH dehydrogenase 1, and the nuclear ribosomal internal transcribed spacer 1. We also took three measurements, maximum length, maximum width, and maximum height, of mussel shells for morphometric analysis.
Molecular and morphological data to resolve species boundaries in the critically imperiled freshwater mussel species, Fusconaia mitchelli
공공데이터포털
We generated multilocus DNA sequence data and traditional morphometric measurements to evaluate species boundaries in Fusconaia mitchelli. We sequenced three loci: the protein-coding mitochondrial DNA genes cytochrome oxidase subunit 1 and NADH dehydrogenase 1, and the nuclear ribosomal internal transcribed spacer 1. We also took three measurements, maximum length, maximum width, and maximum height, of mussel shells for morphometric analysis.