This Data Release makes available measurements of phytoplankton species composition, abundance and cell size made on samples collected in San Francisco Bay (CA) from April 1992 through March 2014. Phytoplankton samples were collected at 31 stations along a 145-km transect where the variability of salinity, temperature, turbidity and nutrient concentrations reflected a broad range of environmental factors that regulate phytoplankton growth and abundance (station map on ScienceBase homepage). Whole water samples were preserved with acid Lugol’s solution, and 2 to 50 ml aliquots were settled in chambers for 6 to 24 hours (Utermöhl 1958). Phytoplankton cells were then counted and identified using a phase-contrast inverted microscope at 125x magnification, all cells greater than 30 μm diameter were enumerated. Cells smaller than 30 μm were counted at 1250x magnification; following the APHA (1989) strip count method, at least 100 cells of the most numerous taxon were counted. Cell volumes were estimated from measured linear dimensions and geometric formulas that varied with cell shapes. Phytoplankton samples representing 923 distinct date, station and depths were included. The 16,442 entries in this dataset include 609 different taxa within 11 functional groups. Diatoms dominated the total biovolume contributed by each functional group (77%) followed by dinoflagellates (14%) and cryptophytes (4.5%). The top 5 species contributing to the summed biovolume in all samples were: Thalassiosira punctigera, Chaetoceros socialis, Ditylum brightwellii, Thalassiosira rotula and Noctiluca scintillans. By frequency of occurrence, the top 5 species were: Teleaulax amphioxeia, Nannochloropsis spp., Plagioselmis prolonga var. nordica, Skeletonema costatum and Thalassiosira eccentrica. APHA. (1989). Standard methods for the examination of water and wastewater, 17th edn. American Public Health Association, Washington, DC Utermöhl H. (1958). Zur Vervollkommnung der quantitativen Phytoplankton-Methodik. Mitt Int Verh Theor Angew Limnol 9:1-38

This Data Release makes available measurements of phytoplankton species composition, abundance and cell size made on samples collected in San Francisco Bay (CA) from April 1992 through March 2014. Phytoplankton samples were collected at 31 stations along a 145-km transect where the variability of salinity, temperature, turbidity and nutrient concentrations reflected a broad range of environmental factors that regulate phytoplankton growth and abundance (station map on ScienceBase homepage). Whole water samples were preserved with acid Lugol’s solution, and 2 to 50 ml aliquots were settled in chambers for 6 to 24 hours (Utermöhl 1958). Phytoplankton cells were then counted and identified using a phase-contrast inverted microscope at 125x magnification, all cells greater than 30 μm diameter were enumerated. Cells smaller than 30 μm were counted at 1250x magnification; following the APHA (1989) strip count method, at least 100 cells of the most numerous taxon were counted. Cell volumes were estimated from measured linear dimensions and geometric formulas that varied with cell shapes. Phytoplankton samples representing 923 distinct date, station and depths were included. The 16,442 entries in this dataset include 609 different taxa within 11 functional groups. Diatoms dominated the total biovolume contributed by each functional group (77%) followed by dinoflagellates (14%) and cryptophytes (4.5%). The top 5 species contributing to the summed biovolume in all samples were: Thalassiosira punctigera, Chaetoceros socialis, Ditylum brightwellii, Thalassiosira rotula and Noctiluca scintillans. By frequency of occurrence, the top 5 species were: Teleaulax amphioxeia, Nannochloropsis spp., Plagioselmis prolonga var. nordica, Skeletonema costatum and Thalassiosira eccentrica. APHA. (1989). Standard methods for the examination of water and wastewater, 17th edn. American Public Health Association, Washington, DC Utermöhl H. (1958). Zur Vervollkommnung der quantitativen Phytoplankton-Methodik. Mitt Int Verh Theor Angew Limnol 9:1-38

This Data Release makes available measurements of phytoplankton species composition, abundance and cell size made on samples collected in San Francisco Bay (CA) from January 2014 through December 2018. Whole water phytoplankton samples were collected at least monthly at fixed sampling stations along a 145-km transect where the variability of salinity, temperature, turbidity and nutrient concentrations reflected a broad range of environmental factors that regulate phytoplankton growth and abundance. A map and table of sampling locations are included in this Data Release. Immediately after samples were collected, they were preserved with acid Lugol’s solution. The samples were analyzed by BSA Environmental Services, Inc following the McNabb (1960) filter method. Following standard methods, at least 400 natural units (colonies, filaments, and unicells) were enumerated to the lowest possible taxonomic level from each sample. In 2014 and 2015, abundances were estimated by random field counts with at least 100 cells of the most numerous taxon counted. From 2016 onward, the 400 natural unit tally was met using two separate random field count efforts: (1) at least 100 natural units of organisms smaller than or equal to 5 μm were identified and enumerated; and (2) at least 300 natural units of organisms larger than 5 μm were identified and enumerated. The 2016 method was adopted to maximize detection of large but rare cells. For all data, cell volumes for each taxon were estimated with the formulas for solid geometric shapes that most closely matched the cell shape according to Hillebrand et al. (1999). Cell volume calculations were based on measurements of up to ten organisms for the most abundant taxa. The Biovolume (cubic microns per ml) of each taxon was computed as the product of abundance (cells per ml) and cell volume (cubic microns per cell). In order to assess replication of results, 10 duplicate samples were collected and analyzed in 2017 and are reported here. Hillebrand, H., Dürselen, C.-D., Kirschtel, D., Pollingher, U. and Zohary, T. 1999. Biovolume Calculation for Pelagic and Benthic Microalgae. Journal of Phycology, 35: 403–424. doi:10.1046/j.1529-8817.1999.3520403.x McNabb, C. D. 1960. Enumeration of freshwater phytoplankton concentrated on the membrane filter. Limnol. Oceanog., 5: 57-61.

This Data Release makes available measurements of phytoplankton species composition, abundance and cell size made on samples collected in San Francisco Bay (CA) from January 2014 through December 2018. Whole water phytoplankton samples were collected at least monthly at fixed sampling stations along a 145-km transect where the variability of salinity, temperature, turbidity and nutrient concentrations reflected a broad range of environmental factors that regulate phytoplankton growth and abundance. A map and table of sampling locations are included in this Data Release. Immediately after samples were collected, they were preserved with acid Lugol’s solution. The samples were analyzed by BSA Environmental Services, Inc following the McNabb (1960) filter method. Following standard methods, at least 400 natural units (colonies, filaments, and unicells) were enumerated to the lowest possible taxonomic level from each sample. In 2014 and 2015, abundances were estimated by random field counts with at least 100 cells of the most numerous taxon counted. From 2016 onward, the 400 natural unit tally was met using two separate random field count efforts: (1) at least 100 natural units of organisms smaller than or equal to 5 μm were identified and enumerated; and (2) at least 300 natural units of organisms larger than 5 μm were identified and enumerated. The 2016 method was adopted to maximize detection of large but rare cells. For all data, cell volumes for each taxon were estimated with the formulas for solid geometric shapes that most closely matched the cell shape according to Hillebrand et al. (1999). Cell volume calculations were based on measurements of up to ten organisms for the most abundant taxa. The Biovolume (cubic microns per ml) of each taxon was computed as the product of abundance (cells per ml) and cell volume (cubic microns per cell). In order to assess replication of results, 10 duplicate samples were collected and analyzed in 2017 and are reported here. Hillebrand, H., Dürselen, C.-D., Kirschtel, D., Pollingher, U. and Zohary, T. 1999. Biovolume Calculation for Pelagic and Benthic Microalgae. Journal of Phycology, 35: 403–424. doi:10.1046/j.1529-8817.1999.3520403.x McNabb, C. D. 1960. Enumeration of freshwater phytoplankton concentrated on the membrane filter. Limnol. Oceanog., 5: 57-61.

This dataset provides the estimated cell abundance and total biovolume for whole-water picoplankton samples collected by the U.S. Geological Survey (USGS) at 25 different sites in Kansas between August of 2020 and August of 2022. The samples were analyzed by BSA Environmental Services, Inc. This data is part of a larger multi-agency project between U.S. Environmental Protection Agency, National Aeronautics and Space Administration, National Oceanic and Atmospheric Administration, and USGS called the Cyanobacteria Assessment Network (CyAN). The goal of the CyAN project is to develop a satellite-based, early warning system to detect harmful algal blooms (HABs) in freshwater systems.

This dataset provides the estimated cell abundance and total biovolume for whole-water picoplankton samples collected by the U.S. Geological Survey (USGS) at 25 different sites in Kansas between August of 2020 and August of 2022. The samples were analyzed by BSA Environmental Services, Inc. This data is part of a larger multi-agency project between U.S. Environmental Protection Agency, National Aeronautics and Space Administration, National Oceanic and Atmospheric Administration, and USGS called the Cyanobacteria Assessment Network (CyAN). The goal of the CyAN project is to develop a satellite-based, early warning system to detect harmful algal blooms (HABs) in freshwater systems.

Depth profiles of phytoplankton community composition collected from a small boat in western Lake Erie in the Great Lakes region from 2017-03-23 to 2022-10-24. These data were collected during repeated trips to a set of stations before, during, and after harmful algal blooms (HABs) using a Fluoroprobe (bbe Moldaenke, GmbH) with factory calibration. Data for the following parameters are included: date and time, green algae, blue-green algae, diatom, cryptophyta, and total chlorophyll concentrations, yellow substances, transmission, depth, and temperature. Any cells in a table with no value means there is no data available. The FluoroProbe provides estimates of phytoplankton concentrations based on total in vivo chlorophyll concentrations. These estimates are based on algorithms derived by bbe Moldaenke. These data represent trends within the phytoplankton assemblage and are not equivalent to extracted chlorophyll concentrations, and should not be considered absolute abundances.

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Ichthyoplankton (biological) data from cruise 0701 from the Yucatan Peninsula, Mexico, Belize, and South Florida. Data is housed in a spreadsheet database (Excel) format for use of members of the Early Life History (ELH) group.

This dataset describes the identification of phytoplankton to the lowest taxonomic level (typically species), as well as abundance (density) and biovolume from grab samples collected from Lake Michigan at Jackson Park at Hyde Park, Illinois and Lake Michigan at Jeorse Park at Gary, Indiana.