This dataset consists of two tables with the results from two manipulative thermal experiments with juvenile coho salmon (May 2021) and juvenile Chinook salmon (May 2020) and include 1) gene transcription results and heat shock protein 70 abundance in coho salmon and Chinook salmon and 2) water temperatures to which fish were exposed during the experiment.

This data set documents the gene transcription levels for a panel of 12 selected genes and the heat shock protein 70 (HSP70) protein abundance measured in the muscle tissue of individual wild Chinook salmon captured from locations within the U.S. portion of the Yukon River watershed. Chinook salmon were primarily captured in 2016 and 2017 from existing field efforts (n = 477). A small number of additional samples (n = 22) were collected in 2018 following an experimental temperature experiment conducted in Pilot Station, AK, USA. During this experiment individual fish were held for ~6 hours at either river ambient control water temperatures or a heated treatment temperature of 18 °C or 21 °C. This small experiment data set was used to validate and refine the use of gene transcription and HSP70 protein levels as heat stress biomarkers for Yukon River Chinook salmon. The analysis of this data was published in von Biela et al. 2020 (DOI:10.1139/cjfas-2020-0209).

This dataset documents the gene transcription levels for a panel of 12 selected genes and the heat shock protein 70 (HSP70) protein abundance measured in the muscle tissue of individual wild Chinook salmon captured from locations within the U.S. portion of the Yukon River watershed. Chinook salmon were primarily captured in 2016 and 2017 from existing field efforts (n = 477). A small number of additional samples (n = 22) were collected in 2018 following an experimental temperature experiment conducted in Pilot Station, Alaska, USA. During this experiment individual fish were held for ~6 hours at either river ambient control water temperatures or a heated treatment temperature of 18 °C or 21 °C. This small experiment dataset was used to validate and refine the use of gene transcription and HSP70 protein levels as heat stress biomarkers for Yukon River Chinook salmon.

This data release contains one table with heat shock protein 70 (HSP70) abundance measurements of Pacific salmon (Oncorhynchus spp.) muscle samples, collected from fish occupying freshwater in various Alaskan watersheds. Water temperatures associated with a sampled fish are also included in the dataset, when available. Gene transcription results from fish included in this data release are published in a separate data release; https://doi.org/10.5066/P19M3AYH.

This data release contains one table with gene transcription results from muscle samples collected from fish occupying freshwater in various Alaskan watersheds. Heat shock protein 70 (HSP70) results from fish included in this data release are published in a separate data release available at https://doi.org/10.5066/P13ZYKWR.

This dataset documents the temperature and dissolved oxygen of water during the implementation of a new experimental thermal challenge protocol for migrating adult Pacific salmon in remote settings. This experiment was conducted with migrating adult Chinook salmon near Pilot Station, Alaska, along the Yukon River in a location without access to utilities.

This data set documents the temperature and dissolved oxygen of water during the implementation of a new experimental thermal challenge protocol for migrating adult Pacific salmon in remote settings. This experiment was conducted with migrating adult Chinook salmon near Pilot Station, Alaska, along the Yukon River in a location without access to utilities. The analysis of this data was published in Donnelly et al. 2020 (DOI:10.1093/conphys/coaa074).

These are genetic data collected from over 700 individual coho salmon (Oncorhynchus kisutch) from 17 streams and rivers within Glacier Bay Alaska and 2 rivers outside the bay. Data collected from all samples include one nuclear gene intron, Growth Hormone-1, and eight microsatellite loci.

These are genetic data collected from over 700 individual coho salmon (Oncorhynchus kisutch) from 17 streams and rivers within Glacier Bay Alaska and 2 rivers outside the bay. Data collected from all samples include one nuclear gene intron, Growth Hormone-1, and eight microsatellite loci.

Closely related species of Salmonidae, including Pacific and Atlantic salmon, can be distinguished from one another based on nucleotide sequences from the cytochrome c oxidase sub-unit 1 mitochondrial gene (CO1), using ensembles of short-reads aligned to genetic barcodes that serve as digital proxies of the relevant species. This is accomplished by exploiting both the nucleotide sequences and their quality scores recorded in a FASTQ file obtained via Next Generation sequencing of mitochondrial DNA extracted from Coho salmon caught with hook and line in the Gulf of Alaska. This subset of the full FASTQ file contains 4839 fragments, each between 100 and 200 nucleotides long, and their associated quality scores. These sequences were used in a novel method for assigning uncertainty to the NGS method employed.