The dataset, which is presented as an Excel spreadsheet, contains all data which is presented as figures in Nichols et al., Measurement of kinetic parameters for biotransformation of polycyclic aromatic hydrocarbons by trout liver S9 fractions: Implications for bioaccumulation assessment, accepted for publication in Applied In Vitro Toxicology 04/2017. Additional information if provided regarding reaction conditions used to characterize liver S9 fractions and perform PAH depletions studies. This dataset is associated with the following publication: Nichols, J., M. Ladd, and P. Fitzsimmons. Measurement of kinetic parameters for biotransformation of polycyclic aromatic hydrocarbons by trout liver S9 fractions: Implications for bioaccumulation assessment. Applied In Vitro Toxicology. Mary Ann Liebert, Inc., Larchmont, NY, USA, 4(4): 365-378, (2018).

This ScienceHub dataset provides characterization data for a pooled sample of trout liver S9 fractions that was used to study the in vitro intrinsic clearance of selected cationic surfactants. These data describe the activity of the pooled sample toward prototypical substrates for cytochrome P450 (CYP) 1A, glutathione-S-transferase, and UDP-glucuronosyltranserase. Also provided are the protein content and total CYP content of the sample and well as information pertaining to the size and gender of fish from which the original sample was obtained. This dataset is associated with the following publication: Droge, S., J. Armitage, J. Arnot, P. Fitzsimmons, and J. Nichols. Biotransformation Potential of Cationic Surfactants in Fish Assessed with Rainbow Trout Liver S9 Fractions. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(11): 3123-3136, (2021).

This ScienceHub dataset provides characterization data for a pooled sample of trout liver S9 fractions that was used to study the in vitro intrinsic clearance of selected cationic surfactants. These data describe the activity of the pooled sample toward prototypical substrates for cytochrome P450 (CYP) 1A, glutathione-S-transferase, and UDP-glucuronosyltranserase. Also provided are the protein content and total CYP content of the sample and well as information pertaining to the size and gender of fish from which the original sample was obtained. This dataset is associated with the following publication: Droge, S., J. Armitage, J. Arnot, P. Fitzsimmons, and J. Nichols. Biotransformation Potential of Cationic Surfactants in Fish Assessed with Rainbow Trout Liver S9 Fractions. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(11): 3123-3136, (2021).

This Excel spreadsheet provides data that appear in Tables 1- 2 and Figures 1-4 of the main document, as well as data that appear in Figures S1-S3 of the Supplementary Information. This dataset is associated with the following publication: Nichols, J., M. Ladd, A. Hoffman, and P. Fitzsimmons. Biotransformation of polycyclic aromatic hydrocarbons by trout liver S9 fractions: Evaluation of competitive inhibition using a substrate depletion approach. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 38(12): 2729-2739, (2019).

This Excel spreadsheet provides data that appear in Tables 1- 2 and Figures 1-4 of the main document, as well as data that appear in Figures S1-S3 of the Supplementary Information. This dataset is associated with the following publication: Nichols, J., M. Ladd, A. Hoffman, and P. Fitzsimmons. Biotransformation of polycyclic aromatic hydrocarbons by trout liver S9 fractions: Evaluation of competitive inhibition using a substrate depletion approach. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 38(12): 2729-2739, (2019).

The purpose of this study was to compare two in vitro systems, cryopreserved trout hepatocytes and trout liver S9 fractions, used to predict in vivo levels of biotransformation in fish. This information is needed to refine modeled estimates of bioaccumulation for hydrophobic organic chemicals that undergo biotransformation. In this effort we used trout hepatocytes to measure in vitro biotransformation of 6 polycyclic aromatic hydrocarbons (PAHs). The results were compared to metabolism rates reported previously for trout liver S9 fractions. Results obtained using both in vitro systems were then used to predict measured levels of hepatic clearance for the same test chemicals exhibited by isolated perfused livers. The results of this study suggest that both in vitro systems are well suited for performing in vitro-in vivo metabolism extrapolations with fish as a means for improving modeled bioaccumulation predictions. This dataset is associated with the following publication: Fay, K., P. Fitzsimmons, A. Hoffman, and J. Nichols. Comparison of trout hepatocytes and liver S9 fractions as in vitro models for predicting hepatic clearance in fish. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, FL, USA, 36(2): 463-471, (2017).

The purpose of this study was to compare two in vitro systems, cryopreserved trout hepatocytes and trout liver S9 fractions, used to predict in vivo levels of biotransformation in fish. This information is needed to refine modeled estimates of bioaccumulation for hydrophobic organic chemicals that undergo biotransformation. In this effort we used trout hepatocytes to measure in vitro biotransformation of 6 polycyclic aromatic hydrocarbons (PAHs). The results were compared to metabolism rates reported previously for trout liver S9 fractions. Results obtained using both in vitro systems were then used to predict measured levels of hepatic clearance for the same test chemicals exhibited by isolated perfused livers. The results of this study suggest that both in vitro systems are well suited for performing in vitro-in vivo metabolism extrapolations with fish as a means for improving modeled bioaccumulation predictions. This dataset is associated with the following publication: Fay, K., P. Fitzsimmons, A. Hoffman, and J. Nichols. Comparison of trout hepatocytes and liver S9 fractions as in vitro models for predicting hepatic clearance in fish. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, FL, USA, 36(2): 463-471, (2017).

This dataset describes the results of a set of experiments, the goal of which was to evaluate the effects of protease inhibitors on the trout liver S9 substrate depletion assay. The presented data show that addition of phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, substantially increases the working lifetime of the trout S9 assay, resulting in improved detection of low intrinsic clearance rates. These findings substantially increase the utility of the trout S9 assay and may have broad implications for its use to support chemical bioaccumulation assessments. This dataset is associated with the following publication: Nichols, J., A. Hoffman, J. Swintek, S. Droge, and P. Fitzsimmons. Addition of phenylmethylsulfonyl fluoride (PMSF) substantially increases the working lifetime of the trout liver S9 substrate depletion assay resulting in improved detection of low intrinsic clearance rates. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(1): 148-161, (2021).

This dataset describes the results of a set of experiments, the goal of which was to evaluate the effects of protease inhibitors on the trout liver S9 substrate depletion assay. The presented data show that addition of phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, substantially increases the working lifetime of the trout S9 assay, resulting in improved detection of low intrinsic clearance rates. These findings substantially increase the utility of the trout S9 assay and may have broad implications for its use to support chemical bioaccumulation assessments. This dataset is associated with the following publication: Nichols, J., A. Hoffman, J. Swintek, S. Droge, and P. Fitzsimmons. Addition of phenylmethylsulfonyl fluoride (PMSF) substantially increases the working lifetime of the trout liver S9 substrate depletion assay resulting in improved detection of low intrinsic clearance rates. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(1): 148-161, (2021).

This dataset illustrates relationships between body weight and hepatic phase I and phase II metabolic activity in rainbow trout. This dataset is associated with the following publication: Fitzsimmons, P., A. Hoffman, K. Fay, and J. Nichols. Allometric scaling of hepatic biotransformation in rainbow trout.. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY PART C: TOXICOLOGY & PHARMACOLOGY. Elsevier Science Ltd, New York, NY, USA, 214: 52-60, (2018).