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Data from: Data on morphological features of mycosis induced by Colletotrichum nymphaeae and Lecanicillium longisporum on citrus orthezia scale
,Symptoms of mycosis induced by two native fungal entomopathogens of the citrus orthezia scale, Praelongorthezia praelonga (Hemiptera: Ortheziidae), an important pest of citrus orchards, are described. The data presented in this article are related to the article entitled "Seasonal prevalence of the insect pathogenic fungus Colletotrichum nymphaeae in Brazilian citrus groves under different chemical pesticide regimes". The endemic fungal pathogen, C. nymphaeae, emerges through the thin cuticular intersegmental regions of the citrus orthezia scale body revealing orange salmon-pigmented conidiophores bearing conidial masses, as well as producing rhizoid-like hyphae that extend over the citrus leaf. By contrast, nymphs or adult females of this scale insect infected with Lecanicillium longisporum exhibit profuse outgrowth of bright white-pigmented conidiophores with clusters of conidia emerging from the insect intersegmental membranes, and mycosed cadavers are commonly observed attached to the leaf surface by hyphal extensions. These morphological differences are important features to discriminate these fungal entomopathogens in citrus orthezia scales.,
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Data From: Two Novel Species of Pestalotiopsis Fungi on Picea and Tsuga from Temperate Forests in the United States
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,In a 2023 survey evaluating conifers with Rosellinia infections, five Pestalotiopsis-like fungal endophytes were isolated from plant samples obtained from Maine, New Hampshire, and Ohio by The Mycology & Nematology Genetic Diversity & Biology Laboratory at the United States Department of Agriculture. The two data sets provided herein contain species-specific base pair substitutions for the partial translation elongation factor 1-alpha gene (TEF). In the alignments, the novel Pestalotiopsis fungi are compared to their most closely related species. The data set can be used as a diagnostic tool to differentiate between the closely related species.,DNA was extracted from fungal samples using the E.Z.N.A HP Plant & Fungal DNA Kit (OMEGA® Bio-Tek, Norcross, GA, USA) following manufacturer’s protocol. The TEF locus was amplified, and reactions were conducted in 25 μL volumes with 12.5 μL of KAPA2G Robust Hotstart® (Kapa Biosystems, Inc., Wilmington, MA, USA), 1.25 μL of the forward and reverse primers at 10 μM, 1-1.75 μL of DNA at 10-20 ng, and 8 μL of molecular grade H2O. Amplifications were performed following the protocol described by Maharachchikumbura et al. (2014) in a C1000 Touch PCR Thermal Cycler (Bio-Rad, Hercules, CA). PCR products were analyzed through capillary electrophoresis with the QIAxcel Advanced System instrument and the QIAxcel ScreenGel software (Qiagen, Hilden, Germany). PCR products were then purified using ExoSAP-IT Cleanup (Affymetrix, Santa Clara, CA) following the manufacturer’s protocol. The BigDye™ 3.1 Terminator Cycle sequencing kit was used to sequence amplicons bi-directionally with the Applied Biosystems SeqStudio Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA).,Resources in this dataset:,,,
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