Everglades National Park (EVER) protects 610,502 ha of natural areas at the extreme southern tip of Florida. EVER consists primarily of shallow seasonal wetland habitats including marshes, sloughs, cypress forests, and mangrove forests. In addition, a portion of the park consists of upland habitat; primarily pine forests and tropical hardwood hammocks. These habitats combined make up a heterogeneous matrix of open grasslands and forested wetlands and uplands. An inventory of amphibian species in Everglades National Park was conducted during 2000 to 2003. The goals of the project were to create a georeferenced inventory of amphibian species, use new analytical techniques to estimate proportion of sites occupied by each species, look for any signs of amphibian decline (missing species, disease, die-offs, etc.), and to establish a protocol that could be used for future monitoring efforts. Primary survey methods were visual encounter surveys (VES) and vocalization surveys but trapping and PVC pipe refugia were used as well.

Bullseye snakehead, Channa marulius, was first detected in 2000 in the southern Florida town of Tamarac and has been expanding its geographic range. Environmental DNA (eDNA) analysis is a newly-developed technique used to non-invasively detect cryptic or low-density species, or those that are logistically difficult to study. Genetic material shed into the environment through tissue and body fluids is concentrated from water samples and analyzed for the presence of target species eDNA. To help delineate bullseye snakehead’s geographic range, we developed and validated a species-specific eDNA assay for both quantitative and droplet digital PCR (ddPCR). We then used ddPCR to assess 16 locations in southeast Florida using 222 water samples collected from 2015-2018. Positive eDNA detections were obtained at all six locations that were within the known geographic range of bullseye snakehead. Furthermore, eDNA was detected in six of 10 locations that were previously thought to be outside the periphery of the range but hydrologically connected through the extensive canal system. Over the four years of sampling, estimated occurrence rates (ψ) remained stable and relatively high (ψ = 0.67 [95% Confidence Interval (CI) 0.33-0.95]) near Tamarac, FL, as compared to the most southern sampling sites (ψ = 0.0-0.37). Bullseye snakehead eDNA estimated occurrence rates in the middle region increased between 2016 (0.28 [95% CI 0.03-0.94]) and 2017 (0.66 [95% CI 0.24-0.98]), potentially reflecting eDNA detections related to a growing or expanding population. Bullseye snakehead eDNA was detected at low concentrations on the northern and eastern borders of Everglades National Park, which is an important conservation area and UNESCO World Heritage Site. Despite extensive sampling via electrofishing, no bullseye snakeheads were visually detected in several locations that yielded positive eDNA samples. It is unclear whether eDNA was transported through flowing water or another vector. To date, collection records for this species are confined to urban canals; however, bullseye snakehead may use the interconnected system of canals to disperse to natural conservation areas, such as Everglades National Park, Big Cypress National Preserve and Water Conservation Areas, where it may impact native species via predation and competition.

American alligators (Alligator mississippiensis) are responsive to hydrologic conditions at all stages of their life history, making them well established indicators of ecosystem restoration within the Everglades. The distribution and success of alligator nesting is monitored annually within Everglades National Park by systematic reconnaissance flight (SRF) helicopter surveys. This dataset represents a subset of the long-term monitoring program data, consisting of data for nests observed between 2008 - 2023, which was used to investigate the relationships between nest-level hydrology and multiple causes of nest failure.

Data were collected between 2010 and 2022 in a research area of the Everglades known as the Decompartmentalization Physical Model (DPM), a wetland area in the central Everglades that includes canals and levees bordering Water Conservation Area 3A (WCA-3A) to the northwest and Water Conservation Area 3B (WCA-3B) to the southeast. During the twelve-year study period more than ten major controlled flow releases occurred by opening the S-152 culverts on canal L-67A that released experimental high flows through the wetland. Data consist of water levels (continuous and discrete), water velocity (continuous and discrete), bed shear stress (discrete), suspended sediment concentration (discrete), dissolved phosphorus concentration, load, and flux (discrete), suspended phosphorus concentration, load and flux (discrete), grainsize distribution (continuous and discrete), related biogeochemistry (discrete), water quality parameters (continuous), temperature profiles (continuous), microtopography (discrete), and vegetation community type and stem density (discrete).

This data was collected as part of a sampling protocol to monitor ecotonal movement of mangroves and upslope freshwater marshes in Everglades National Park and Big Cypress National Preserve. Remote sensing approaches are used to delineate the physical location of the ecotone (boundaries) between mangroves and freshwater marsh. Fourteen 3 kilometer (1.9 mile [mi]) segments are systematically placed along the ecotone within the two parks. Aerial imagery is used to digitize the mangrove-marsh ecotone in each segment and must be 1 meter (3.3 feet [ft]) or better resolution and have positional accuracy of ± 3 meters (9.8 feet [ft]) or better. Four ground-truth field point locations (marker points) are established approximately 1 kilometer (0.62 miles [mi]) apart along the ecotone of each segment to provide field-based measurements and ground-truthing of the digitized line. Vegetation data is collected at each sampling point, with this vegetation coverage determining where the placement of the ecotone should be. generally, this is where mangrove cover drops below 50% cover. The vegetation data also verifies if it is mangroves (typical Red) that is the dominant tree on the tress side of the ecotonal edge. The vegetation data also indicated the dominant graminoid on the more inland side of the ecotone. We have found 3 major groups for the marsh dominant vegetation type. This could be important mitigating factor or a indicator of future conditions. NOTE - Data for this data release was pulled from the mangrove marsh ecotone database and preliminarily cleaned and organized in May 2025. The data should undergo further QAQC before being used in analysis.

Quantitative waterbird surveys over seasonal wetlands of the Alligator Rivers Region (ARR) of the NT, provided by the Supervising Scientist Division (SSD) of the Commonwealth Department of the Environment, Water, Heritage and the Arts (DEWHA). Ground and aerial surveys at 17 billabongs within the Magela Creek catchment. Environmental information includes structural classification of each billabong surveyed

Quantitative waterbird surveys over seasonal wetlands of the Alligator Rivers Region (ARR) of the NT, provided by the Supervising Scientist Division (SSD) of the Commonwealth Department of the Environment, Water, Heritage and the Arts (DEWHA). Systematic ground surveys from 30 sites on the Magela Floodplain, Kakadu National Park

This data release includes 2 child items with tracking data for Whimbrels, a shorebird found throughout tundra and boreal habitats of Alaska during the summer and winters along the coasts of the continental U.S., and central and South America. Child Item 1: "Argos Satellite Tracking Data for Whimbrels (Numenius phaeopus) - Processed Data" -- Quality-controlled data collected from Argos satellite transmitters. Child Item 2: "Argos Satellite Tracking Data for Whimbrels (Numenius phaeopus) - Raw Data" -- All raw data collected from Argos satellite transmitters, provided for completeness of the archive. The quality-controlled, "Argos Processed Data" (Child Item 1) are better suited for most analytical purposes.

This volume's release consists of 90364 media files captured by autonomous wildlife monitoring devices under the project, Silvio O Conte National Fish and Wildlife Refuge Wildlife Monitoring Project. The attached files listed below include several CSV files that provide information about the data release. The file, "media.csv" provides the metadata about the media, such as filename and date/time of capture. The actual media files are housed within folders under the volume's "child items" as compressed files. A critical CSV file is "dictionary.csv", which describes each CSV file, including field names, data types, descriptions, and the relationship of each field to fields in other CSV files. Some of the media files may have been "tagged" or "annotated" by either humans or by machine learning models, identifying wildlife targets within the media. If so, this information is stored in "annotations.csv" and "modeloutputs.csv", respectively. To protect privacy, all personally identifiable information (PII) have been removed, locations have been "blurred" by bounding boxes, and media featuring sensitive taxa or humans have been omitted. To enhance data reuse, the sbRehydrate() function in the AMMonitor R package will download files and re-create the original AMMonitor project (database + media files). See source code at https://code.usgs.gov/vtcfwru/ammonitor.

Quantitative PCR results for surveillance of New Zealand mudsnail environmental DNA at the U.S. Fish and Wildlife Service Alchesay National Fish Hatchery.