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Microsatellite genotypes and DNA yield of artificially produced larval pallid sturgeon, Scaphirhynchus albus
Data consist of genotypes for pallid sturgeon and paddlefish broodstock and artificially-produced larvae at 19 sturgeon microsatellite loci and four paddlefish loci. Microsatellite genotypes allowed confirmation of homozygous sturgeon DNA and absence of paddlefish DNA in haploid gynogens. Data also consist of DNA yield estimates for haploid gynogens for proof of concept that haploid gynogens can provide sufficient DNA quantity for restriction site-associated DNA sequencing studies.
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Ploidy and genome size estimates of artificially produced larval pallid sturgeon, Scaphirhynchus albus
공공데이터포털
Data consist of flow cytometry files (.FCS) that were generated in the analysis of larval pallid sturgeon specimens. Nuclei from produced larval sturgeon somatic cells were analyzed using flow cytometry to estimate genome size and assign ploidy. Freshly collected rainbow trout (Oncorhynchus mykiss), largemouth bass (Micropterus salmoides), or pallid sturgeon blood were used as external controls, whereby the fish blood samples were analyzed in separate tubes from the cells of prepared sturgeon larvae. All samples at 1 x 10^6 cells/mL were incubated in a propidium iodide solution to stain DNA, then analyzed by flow cytometry. Analysis software generated histograms from the experimental samples and the control standard samples. The histogram peak channels (modes) were then compared to generate a genome size estimate and additionally to visualize peaks for ploidy categorization.
Ploidy and genome size estimates of artificially produced larval pallid sturgeon, Scaphirhynchus albus
공공데이터포털
Data consist of flow cytometry files (.FCS) that were generated in the analysis of larval pallid sturgeon specimens. Nuclei from produced larval sturgeon somatic cells were analyzed using flow cytometry to estimate genome size and assign ploidy. Freshly collected rainbow trout (Oncorhynchus mykiss), largemouth bass (Micropterus salmoides), or pallid sturgeon blood were used as external controls, whereby the fish blood samples were analyzed in separate tubes from the cells of prepared sturgeon larvae. All samples at 1 x 10^6 cells/mL were incubated in a propidium iodide solution to stain DNA, then analyzed by flow cytometry. Analysis software generated histograms from the experimental samples and the control standard samples. The histogram peak channels (modes) were then compared to generate a genome size estimate and additionally to visualize peaks for ploidy categorization.
Individual assignments and microsatellite genotypes for Atlantic Sturgeon from 2021 (ver. 4.0, September 2025)
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This dataset includes genotypes and individual-based assignment tests for 1394 Atlantic Sturgeon selected for assignment by the National Marine Fisheries Service to meet management requirements under the Endangered Species Act.
Individual assignments and microsatellite genotypes for Atlantic Sturgeon from 2021 (ver. 4.0, September 2025)
공공데이터포털
This dataset includes genotypes and individual-based assignment tests for 1026 Atlantic Sturgeon selected for assignment by the National Marine Fisheries Service to meet management requirements under the Endangered Species Act.
Individual assignments and microsatellite genotypes for Atlantic Sturgeon from 2021 (ver. 3.0, January 2025)
공공데이터포털
This dataset includes genotypes and individual-based assignment tests for 1026 Atlantic Sturgeon selected for assignment by the National Marine Fisheries Service to meet management requirements under the Endangered Species Act.
Genotypes for Atlantic Sturgeon Included in Assignment Tests
공공데이터포털
Genotypes for Atlantic Sturgeon that were submitted by the National Marine Fisheries Service from individuals encountered as mortalities or taken during permitted activities.
Genotypes for Atlantic Sturgeon Included in Assignment Tests
공공데이터포털
Genotypes for Atlantic Sturgeon that were submitted by the National Marine Fisheries Service from individuals encountered as mortalities or taken during permitted activities.
Results of individual-based genetic assignments for Atlantic sturgeon
공공데이터포털
Data represent results of individual-based assignment tests performed in the program GeneClass for Atlantic Sturgeon of unknown origin. Data for genetic sex are also presented.