This file contains data used to generate figures shown in Weitekamp et al. Triclosan-resistant host-associated microbiota perform xenobiotic biotransformations in larval zebrafish. This dataset is associated with the following publication: Weitekamp, C., D. Phelps, A. Swank, J. McCord, J. Sobus, T. Catron, S. Keely, N. Brinkman, T. Zurlinden, E. Wheaton, M. Strynar, C. McQueen, C. Wood, and T. Tal. Triclosan-Selected Host-Associated Microbiota Perform Xenobiotic Biotransformations in Larval Zebrafish (2019). TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 172(1): 109-122, (2019).

This file contains data used to generate figures shown in Catron et al. Characterization of host toxicity and microbiota disruption in larval zebrafish following developmental exposure to BPA and BPA alternatives. This dataset is associated with the following publication: Catron, T., S. Keely, N. Brinkman, T. Zurlinden, C. Wood, J. Wright, D. Phelps, E. Wheaton, A. Kvasnicka, S. Gaballah, R. Lamendella, and T. Tal. Host Developmental Toxicity of BPA and BPA Alternatives Is Inversely Related to Microbiota Disruption in Zebrafish. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 167(2): 468-483, (2019).

Across taxa, animals with depleted intestinal microbiomes show disrupted behavioral phenotypes. Axenic (i.e., microbe-free) mice, zebrafish, and fruit flies exhibit increased locomotor behavior, or hyperactivity. The mechanism through which bacteria interact with host cells to trigger normal neurobehavioral development in larval zebrafish is unknown. Here, we monoassociated zebrafish with either one of six different zebrafish-associated bacteria, mixtures of these host-associates, or with an environmental bacterial isolate. We found that while the axenic cohort was hyperactive, monoassociation with three different host-associated bacterial species, as well as with the mixtures, resulted in control-like locomotor behavior. Monoassociation with one host-associate and the environmental isolate resulted in the hyperactive phenotype characteristic of axenic larvae, while monoassociation with two other host-associated bacteria partially blocked this phenotype. Furthermore, we found an intriguing inverse relationship between the total concentration of bacteria per larvae and locomotor behavior. These data support a growing body of evidence that individual species of bacteria can have different effects on host behavior, potentially related to their success at intestinal colonization. Specific to the zebrafish model, our results suggest that differences in the composition of microbes in fish facilities could have profound effects on the outcomes of behavioral and pharmacological studies. This dataset is associated with the following publication: Weitekamp, C., A. Kvasnicka, S. Keely, N. Brinkman, X. Howey, S. Gaballah, D. Phelps, T. Catron, T. Zurlinden, E. Wheaton, and T. Tal. Monoassociation with bacterial isolates reveals the role of colonization, community complexity and abundance on locomotor behavior in larval zebrafish. Animal Microbiome. BioMed Central Ltd, London, UK, 3(12): 1-13, (2021).

Effects of developmental 17beta-estradiol exposure on microbiota and behavior in zebrafish. This dataset is associated with the following publication: Catron, T., A. Swank, L. Wehmas, D. Phelps, S. Keely, N. Brinkman, J. McCord, R. Singh, J. Sobus, C. Wood, M. Strynar, E. Wheaton, and T. Tal. Microbiota alter metabolism and mediate neurodevelopmental toxicity of 17β-estradiol. Scientific Reports. Nature Publishing Group, London, UK, 9: Article number 7064, (2019).

Zebra mussels (Dreissena polymorpha Pallas 1771) are a tenacious aquatic invasive species in the United States and new infestations can rapidly expand into dense colonies. Zebra mussels were first reported in Marion Lake (Dakota County, Minnesota) in September 2017 and surveys indicated the infestation was likely isolated near the public boat access. A 2.4-hectare area, containing the known zebra mussel infestation, was enclosed and treated for nine days with EarthTec QZ®, a copper-based molluscicide, in an attempt to eradicate the zebra mussels. We conducted an on-site bioassay to confirm treatment efficacy. The bioassay was conducted in mobile assay trailer that received a continuous flow of treated lake water and zebra mussels test animals that were collected from White Bear Lake (Ramsey County, MN). Zebra mussel mortality in the treated bioassay tanks achieved 99 percent mortality (95-percent Confidence Interval: 98 – 100 percent) and a predictive model indicates in-lake mortality as low as 85 percent may have occurred; therefore, our results indicate that the Marion Lake treatment may not have induced complete zebra mussel mortality. Post-treatment assessments and surveys are recommended to further verify treatment success and monitor lake conditions in regards to zebra mussel infestation.

Zebra mussels (Dreissena polymorpha Pallas 1771) are a tenacious aquatic invasive species in the United States and new infestations can rapidly expand into dense colonies. Zebra mussels were first reported in Marion Lake (Dakota County, Minnesota) in September 2017 and surveys indicated the infestation was likely isolated near the public boat access. A 2.4-hectare area, containing the known zebra mussel infestation, was enclosed and treated for nine days with EarthTec QZ®, a copper-based molluscicide, in an attempt to eradicate the zebra mussels. We conducted an on-site bioassay to confirm treatment efficacy. The bioassay was conducted in mobile assay trailer that received a continuous flow of treated lake water and zebra mussels test animals that were collected from White Bear Lake (Ramsey County, MN). Zebra mussel mortality in the treated bioassay tanks achieved 99 percent mortality (95-percent Confidence Interval: 98 – 100 percent) and a predictive model indicates in-lake mortality as low as 85 percent may have occurred; therefore, our results indicate that the Marion Lake treatment may not have induced complete zebra mussel mortality. Post-treatment assessments and surveys are recommended to further verify treatment success and monitor lake conditions in regards to zebra mussel infestation.

Data used to generate figures shown in Phelps et al. This dataset is associated with the following publication: Phelps, D., N. Brinkman, S. Keely, E. Anneken, T. Catron, D. Betancourt, C. Wood, S. Espenschied, J. Rawls, and T. Tal. Microbial colonization is required for normal neurobehavioral development in zebrafish. Scientific Reports. Nature Publishing Group, London, UK, 11(7): 11244, (2017).

Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways with early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous eradication treatments have been conducted during times of variable water temperatures and temperature has been shown to influence the efficacy of molluscicides. Natural resource managers would benefit from knowledge regarding the impacts of water temperature and exposure duration on toxicity of molluscicides to zebra mussels. In particular, temperature specific data are needed to inform the selection of an effective molluscicide and the proper dose that will induce 100% zebra mussel mortality. We evaluated the influences of temperature and exposure duration on the toxicity of two EPA-registered (EarthTec QZ and Zequanox) and two nonregistered (niclosamide and potassium chloride) molluscicides to zebra mussels at water temperatures of 7, 12, 17, and 22 °C. Our results indicate that treatment options for the eradication of zebra mussels in waters ≤ 12 °C include 336 h or longer treatments with EarthTec QZ and KCl and treatments with niclosamide ≥ 24 h in duration. In waters ≥ 17 °C, multiple toxicant and exposure duration combinations would be effective for zebra mussel eradication treatments. However, site specific variables should be considered prior to treatment including: the extent of the infestation, water chemistry, aquatic vegetation, substrate, and the presence of nontarget organisms. The use of on-site or in situ zebra mussel bioassays would also be a useful tool for the evaluation of treatment efficacy. The dataset includes: Water Quality, Chemical Concentrations, Mortality, and Zebra Mussel Condition Data

Zebra mussels (Dreissena polymorpha) have continued their spread within inland lakes and rivers in North America despite diligent containment and decontamination efforts by natural resource agencies and other stakeholders. Identification of newly infested waterways with early detection surveillance programs allows for rapid response zebra mussel eradication treatments in some situations. Previous eradication treatments have been conducted during times of variable water temperatures and temperature has been shown to influence the efficacy of molluscicides. Natural resource managers would benefit from knowledge regarding the impacts of water temperature and exposure duration on toxicity of molluscicides to zebra mussels. In particular, temperature specific data are needed to inform the selection of an effective molluscicide and the proper dose that will induce 100% zebra mussel mortality. We evaluated the influences of temperature and exposure duration on the toxicity of two EPA-registered (EarthTec QZ and Zequanox) and two nonregistered (niclosamide and potassium chloride) molluscicides to zebra mussels at water temperatures of 7, 12, 17, and 22 °C. Our results indicate that treatment options for the eradication of zebra mussels in waters ≤ 12 °C include 336 h or longer treatments with EarthTec QZ and KCl and treatments with niclosamide ≥ 24 h in duration. In waters ≥ 17 °C, multiple toxicant and exposure duration combinations would be effective for zebra mussel eradication treatments. However, site specific variables should be considered prior to treatment including: the extent of the infestation, water chemistry, aquatic vegetation, substrate, and the presence of nontarget organisms. The use of on-site or in situ zebra mussel bioassays would also be a useful tool for the evaluation of treatment efficacy. The dataset includes: Water Quality, Chemical Concentrations, Mortality, and Zebra Mussel Condition Data

The efficacy and subsurface application of Zequanox®, a commercially prepared spray-dried powder formulation of Pseudomonas fluorescens (strain CL145A), were evaluated for controlling zebra mussels (Dreissena polymorpha) within 27-m2 enclosures in Lake Minnetonka (Deephaven, Minnesota). Five treatments consisting of (1) two whole water column Zequanox applications, (2) two subsurface Zequanox applications, and (3) an untreated control were completed on each of three independent treatment days during September 2014. The two types of samplers used in the study were (1) custom built multi-plate samplers (type 1 samplers), with wood, perforated aluminum, and tile substrates that were placed into Robinson’s Bay in June of 2013 to allow for natural colonization by zebra mussels, and (2) samplers that were designed to contain zebra mussels (type 2 samplers) which consisted of zebra mussels adhering to perforated aluminum trays that were placed into mesh containment bags. One day prior to treatment, three individual samplers of each type were distributed to test enclosures and exposed to a randomly assigned treatment. Sampling to determine the living zebra mussel biomass adhering to type 1 samplers and the survival assessments for zebra mussels contained in type 2 samplers were completed ~40 days after exposure. The living zebra mussel biomass adhering to type 1 samplers and the survival of zebra mussels contained in type 2 samplers were significantly less in groups treated with the highest Zequanox concentrations and in groups that received whole water column applications compared to groups treated with lower Zequanox concentrations and subsurface applications. However, standardization biomass and survival results to the amount of Zequanox applied showed that the lower Zequanox concentrations and subsurface applications were more efficient at reducing zebra mussel biomass and inducing zebra mussel mortality. Although more efficient, biological significance and management goals should be evaluated prior to selecting subsurface application methods and lower treatment concentrations for Zequanox applications. Development and refinement of additional application techniques may improve the utility of the subsurface Zequanox applications.