This study aimed to (1) enrich microbial acetylenotrophs from trichloroethylene (TCE) contaminated groundwater and (2) evaluate whether these enrichments could degrade TCE coupled to acetylene degradation. Acetylenotrophs are microorganisms that use acetylene as their carbon and energy source. TCE contaminated groundwater was collected from wells at the Naval Air Warfare Center (NAWC) in West Trenton, New Jersey. Microbial acetylene uptake in groundwater samples was established by mixing the groundwater with a defined mineral medium to supply nutrients and providing acetylene as the sole electron donor and carbon source. The structure of the microbial community in those enrichments was characterized as shown by 16S rRNA gene sequencing and analysis. The acetylenotrophic groundwater enrichment cultures were then tested to assess whether they could utilize acetylene to drive reduction of TCE and tetrachloroethene (PCE) to vinyl chloride.

This U.S. Geological Survey (USGS) Data Release provides water-quality data collected during 1-week and 2-week nutrient studies beginning on February 22, May 17, and July 26 located in the Caloosahatchee River at the S-79 water-control structure. For each study period, 12 fiberglass tanks were suspended in the river using three floating cradles with each cradle holding four tanks. Each tank was open to the atmosphere and closed to the river. The tanks were filled with native water during the first day of each study period. Four different treatment methods were represented within each cradle. For all three study periods, two of the four treatments remained constant: ammonium hydroxide and untreated. For the 1-week study period in February, the additional treatment methods were sodium phosphate dibasic dodecahydrate and sodium nitrate. For the 2-week study period in May, the additional treatment methods were sodium phosphate dibasic dodecahydrate and urea. For the 2-week study period during July-August, the additional treatment methods were sodium nitrate and urea. Treatments were added to the tanks following the profiles on the first day of each study period. Nutrient samples were collected and processed by Nova Southeastern University. Water-quality sensor data were collected near the surface (approximately 1 foot below the water surface), near the middle of the water column (approximately 2 feet below the water surface), and near the bottom (approximately 3 feet below the water surface) of each tank using a multi-parameter water-quality sonde. Additional water-quality sensor data were collected at approximately 1, 2, and 3 feet below the water surface within the river. Each point reading is provided as an instantaneous measurement. Water-quality parameters measured include water temperature, specific conductance, pH, dissolved oxygen, turbidity, phycocyanin fluorescence, chlorophyll fluorescence, and fluorescence of dissolved organic matter. Revision History: First release: November 2023. Version 1.1: August 2024. Changes from previous version: in file "Mesocosms_profiles_2021_datarelease_data" revised one of the four treatment methods for the July-August study period from sodium phosphate dibasic dodecahydrate to sodium nitrate to correct an Excel entry error.

The dataset includes water-quality sensor readings collected by the U.S Geological Survey (USGS) from the Caloosahatchee River at the Franklin Lock and Dam and 12 open-air fiberglass tanks filled with Caloosahatchee River water used for mesocosm experiments testing the effects of elevated nutrients on harmful algal bloom (HAB) dynamics. This dataset contains water quality sensor readings from two of eight total independent experiments conducted from June 8-11, 2020 and September 14-17, 2020. Each of the 12 tanks were randomly treated with either ammonium hydroxide, sodium nitrate, sodium phosphate, or left untreated (controls) for a total of three replicates of each treatment. The tanks were treated with incrementally higher dosing solutions every 24 hours for the first three days of the four-day experiment (T0, T24, and T48). Biological and nutrient concentration samples were collected each day before and after the dosing solution was applied and at T72, the final day of the experiment. Water-quality sensor data were collected on all four days (T0, T24, T48, T72) at three depths within each tank and the river using a multi-parameter water-quality sonde before the dosing solutions were applied. Each point reading is provided as an instantaneous measurement. Water-quality parameters measured include chlorophyll fluorescence, dissolved oxygen, fluorescence of dissolved organic matter, pH, phycocyanin fluorescence, specific conductance, turbidity, and water temperature. The data is provided as a table in comma delimited format.

This U.S. Geological Survey (USGS) Data Release provides water-quality data collected during 1-week and 2-week nutrient studies beginning on February 22, May 17, and July 26 located in the Caloosahatchee River at the S-79 water-control structure. For each study period, 12 fiberglass tanks were suspended in the river using three floating cradles with each cradle holding four tanks. Each tank was open to the atmosphere and closed to the river. The tanks were filled with native water during the first day of each study period. Four different treatment methods were represented within each cradle. For all three study periods, two of the four treatments remained constant: ammonium hydroxide and untreated. For the 1-week study period in February, the additional treatment methods were sodium phosphate dibasic dodecahydrate and sodium nitrate. For the 2-week study period in May, the additional treatment methods were sodium phosphate dibasic dodecahydrate and urea. For the 2-week study period during July-August, the additional treatment methods were sodium nitrate and urea. Treatments were added to the tanks following the profiles on the first day of each study period. Nutrient samples were collected and processed by Nova Southeastern University. Water-quality sensor data were collected near the surface (approximately 1 foot below the water surface), near the middle of the water column (approximately 2 feet below the water surface), and near the bottom (approximately 3 feet below the water surface) of each tank using a multi-parameter water-quality sonde. Additional water-quality sensor data were collected at approximately 1, 2, and 3 feet below the water surface within the river. Each point reading is provided as an instantaneous measurement. Water-quality parameters measured include water temperature, specific conductance, pH, dissolved oxygen, turbidity, phycocyanin fluorescence, chlorophyll fluorescence, and fluorescence of dissolved organic matter. Revision History: First release: November 2023. Version 1.1: August 2024. Changes from previous version: in file "Mesocosms_profiles_2021_datarelease_data" revised one of the four treatment methods for the July-August study period from sodium phosphate dibasic dodecahydrate to sodium nitrate to correct an Excel entry error.

Sediment accumulation in water storage tanks may protect microorganisms from disinfectant exposure, causing water quality degradation. However, microbial activity and disinfectant penetration within water storage sediment remains largely uncharacterized. This study evaluated monochloramine and free chlorine penetration into a 2-cm (20,000 µm) deep drinking water storage tank sediment using microelectrodes. The sediment was successively exposed to 4-months monochloramine, 2-months free chlorine, and 2-months monochloramine. Temporal monochloramine, free chlorine, dissolved oxygen (DO), pH, ammonium, nitrite, and nitrate profiles were acquired using microelectrodes. Results showed that complete monochloramine or free chlorine penetration was not observed. Likewise, DO never fully penetrated the sediment, progressing inward with time to a maximum depth of 10,000 µm and indicating microbial activity remained during the entire 8 months. Decreasing ammonium and increasing nitrate concentrations, with minimal nitrite accumulation, further demonstrated microbial activity and indicated complete sediment nitrification. There was measurable ammonium, nitrite, and nitrate during free chlorine application and nitrification activity gradually resumed upon a switch back to monochloramine. These findings suggest that periodic sediment removal from drinking water storage facilities is desirable to remove potentially protected environments for microorganisms. This dataset is associated with the following publication: Liu, H., D. Wahman, and J. Pressman. Evaluation of Monochloramine and Free Chlorine Penetration in a Drinking Water Storage Tank Sediment Using Microelectrodes. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 53(16): 9352-9360, (2019).

Sediment accumulation in water storage tanks may protect microorganisms from disinfectant exposure, causing water quality degradation. However, microbial activity and disinfectant penetration within water storage sediment remains largely uncharacterized. This study evaluated monochloramine and free chlorine penetration into a 2-cm (20,000 µm) deep drinking water storage tank sediment using microelectrodes. The sediment was successively exposed to 4-months monochloramine, 2-months free chlorine, and 2-months monochloramine. Temporal monochloramine, free chlorine, dissolved oxygen (DO), pH, ammonium, nitrite, and nitrate profiles were acquired using microelectrodes. Results showed that complete monochloramine or free chlorine penetration was not observed. Likewise, DO never fully penetrated the sediment, progressing inward with time to a maximum depth of 10,000 µm and indicating microbial activity remained during the entire 8 months. Decreasing ammonium and increasing nitrate concentrations, with minimal nitrite accumulation, further demonstrated microbial activity and indicated complete sediment nitrification. There was measurable ammonium, nitrite, and nitrate during free chlorine application and nitrification activity gradually resumed upon a switch back to monochloramine. These findings suggest that periodic sediment removal from drinking water storage facilities is desirable to remove potentially protected environments for microorganisms. This dataset is associated with the following publication: Liu, H., D. Wahman, and J. Pressman. Evaluation of Monochloramine and Free Chlorine Penetration in a Drinking Water Storage Tank Sediment Using Microelectrodes. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 53(16): 9352-9360, (2019).

The bacteria sequence data generated in this study is available in the Short Read Archive (SRA) of NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA509718. This dataset is associated with the following publication: Siponen, S., J. Ikonen, V. Gomez-Alvarez, A. Hokajärvi, M. Ruokolainen, B. Jayaprakash, M. Kolehmainen, I.T. Miettinen, T. Pitkänen, and E. Torvinen. Effect of Pipe Material and Disinfectant on Active Bacterial Communities in Drinking Water and Biofilms. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 136(1): lxaf004, (2025).

This study examined the removal of chloroform under two environmental conditions (anaerobic and aerobic), and in the presence of ethanol as co-metabolite. Investigations of the biological community structure within the BTFs were also conducted. The use of aerobic fungi BTF under acidic condition successfully enhanced the biodegradation process of chloroform. The BTF provided more stable performance by having smaller standard deviation in the removal efficiency as compared to the anaerobic BTF. Hence, acidic aerobic BTF had achieved significant improvement in the removal of chloroform. This dataset is associated with the following publication: Sahle-Demessie, E., J. Lu, B. Mezgebe, and G. Sorial. Performance of Anaerobic Biotrickling Filter and Its Microbial Diversity for the Removal of Stripped Disinfection By-products. INTERNATIONAL JOURNAL OF ENVIRONMENTAL POLLUTION. Universidad Nacional Autonoma de Mexico, 228: 437, (2017).

This study examined the removal of chloroform under two environmental conditions (anaerobic and aerobic), and in the presence of ethanol as co-metabolite. Investigations of the biological community structure within the BTFs were also conducted. The use of aerobic fungi BTF under acidic condition successfully enhanced the biodegradation process of chloroform. The BTF provided more stable performance by having smaller standard deviation in the removal efficiency as compared to the anaerobic BTF. Hence, acidic aerobic BTF had achieved significant improvement in the removal of chloroform. This dataset is associated with the following publication: Sahle-Demessie, E., J. Lu, B. Mezgebe, and G. Sorial. Performance of Anaerobic Biotrickling Filter and Its Microbial Diversity for the Removal of Stripped Disinfection By-products. INTERNATIONAL JOURNAL OF ENVIRONMENTAL POLLUTION. Universidad Nacional Autonoma de Mexico, 228: 437, (2017).

This U.S. Geological Survey (USGS) Data Release provides water-quality data collected from the Caloosahatchee River and 12 fiberglass tanks located within the Caloosahatchee River. The tanks were open to the atmosphere, and were closed to the river. Tanks were filled with native water within 1-2 hours prior to the first profile collected on May 6, July 8, and September 16. Nutrients were added at approximately 12:00 p.m. on May 6, 11:45 a.m. on July 8, and 11:00 a.m. on September 16. Sodium nitrate was added for the nitrate treatments, sodium phosphate was added for the phosphate treatments, and ammonium hydroxide was added for the ammonium treatments. Nutrient samples were collected and processed by Nova Southeastern University. Water-quality measurements were made at 3 depths within each fiberglass tank, near the surface (approximately 1 foot deep), near the middle of the water column, (approximately 2 feet deep) and near the bottom (approximately 3 feet deep), and at approximately 1, 2, and 3 feet deep within the river itself. Each depth location value represents an approximate 30 second average. Water-quality characteristics measured and recorded include water temperature, specific conductance, dissolved oxygen, pH, turbidity, chlorophyll fluorescence, phycocyanin fluorescence, and fluorescence of dissolved organic matter.