This dataset characterizes sensitivity of fathead minnow and a unionid mussel to elevated major ions in produced water from oil and gas extraction process.

The data release includes data from four studies: (1) toxicity of a permitted effluent, which entered the Deep Fork River (DFR), Oklahoma, USA, to a unionid mussel (Lampsilis siliquoidea) and to 2 standard test species (cladoceran Ceriodaphnia dubia; and fathead minnow Pimephales promelas) in short-term 7-d effluent tests; (2) relative sensitivities of the 3 species to potassium (K), an elevated major ion in the effluent, using 7-d toxicity tests with KCl spiked into a DFR upstream reference water; (3) potential influences of background water characteristics on the acute K toxicity to the mussel (96-h exposures) and cladoceran (48-h exposure) in 4 reconstituted waters that mimicked the hardness and ionic composition ranges of the DFR; and (4) potential influence of temperature on acute K toxicity to the mussel. Water quality, survival, growth, and reproduction endpoints are reported.

The data release includes data from four studies: (1) toxicity of a permitted effluent, which entered the Deep Fork River (DFR), Oklahoma, USA, to a unionid mussel (Lampsilis siliquoidea) and to 2 standard test species (cladoceran Ceriodaphnia dubia; and fathead minnow Pimephales promelas) in short-term 7-d effluent tests; (2) relative sensitivities of the 3 species to potassium (K), an elevated major ion in the effluent, using 7-d toxicity tests with KCl spiked into a DFR upstream reference water; (3) potential influences of background water characteristics on the acute K toxicity to the mussel (96-h exposures) and cladoceran (48-h exposure) in 4 reconstituted waters that mimicked the hardness and ionic composition ranges of the DFR; and (4) potential influence of temperature on acute K toxicity to the mussel. Water quality, survival, growth, and reproduction endpoints are reported.

Supplementary data for "Leveraging invasive mussel contaminant survey data for stepwise prioritization of chemicals of potential concern in the Great Lakes basin". This dataset is associated with the following publication: Fuller, N., K. Kimbrough, M. Edwards, E. Maloney, S. Corsi, M. Pronschinske, L. DeCicco, J. Frisch, A. Baldwin, S. Hummel, N. Garcia Reyero, and D. Villeneuve. Leveraging invasive mussel contaminant survey data for stepwise prioritization of chemicals of potential concern in the Great Lakes basin. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 44(7): 2070-2087, (2025).

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

Attributes regarding the acute toxicity of sodium chloride (NaCl) and potassium chloride (KCl) to larvae (glochidia) or juveniles of a unionid mussel (fatmucket, Lampsilis siliquoidea) at various water hardness and in the presence of other major ions.

Attributes regarding the acute toxicity of sodium chloride (NaCl) and potassium chloride (KCl) to larvae (glochidia) or juveniles of a unionid mussel (fatmucket, Lampsilis siliquoidea) at various water hardness and in the presence of other major ions.

Additional data generated during the present study are available as a US Geological Survey data release at https://doi.org/XXXX (pending). This dataset is associated with the following publication: Wang, N., J. Kunz, D. Cleveland, R. Dorman, J. Steevens, S. Raimondo, T. Augsberger, and C. Barnhardt. Evaluation of Chronic Effects of Potassium Chloride and Nickel on Survival, Growth, and Reproduction of a Unionid Mussel (Lampsilis siliquoidea). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 43(5): 1097–1111, (2024).

High-resolution mass spectrometry is advantageous for monitoring physiological impacts and contaminant biotransformation products in fish exposed to complex wastewater effluent. We evaluated this technique using skin mucus from male and female fathead minnows (Pimephales promelas) exposed to control water or treated wastewater effluent at 5%, 20%, and 100% levels for 21 d, using an onsite, flow-through system providing real-time exposure. Both sex-specific and non-sex-specific responses were observed in the mucus metabolome, the latter suggesting the induction of general compensatory pathways for xenobiotic exposures. Altogether, 85 statistically significant treatment-dependent metabolite changes were observed and 30 of those annotated with probable structures. The mummichog software package was used to elucidate impacted biochemical pathways and enhance metabolite annotation. Partial least squares regression models revealed relationships between the mucus metabolomes and upregulated hepatic mRNA transcripts reported previously for these same fish. These regression models suggest that mucus metabolomic changes reflected, in part, processes by which the fish biotransformed xenobiotics in the effluent. Further, we detected a phase II transformation product of bisphenol A in the skin mucus of male fish. Collectively, these findings demonstrate the utility of mucus as a minimally invasive matrix for simultaneously assessing exposures and effects of real-world mixtures of contaminants. This dataset is associated with the following publication: Mosley, J., D. Ekman, J.E. Cavallin, D. Villeneuve, G. Ankley, and T. Collette. High‐resolution mass spectrometry of skin mucus for monitoring physiological impacts and contaminant biotransformation products in fathead minnows exposed to wastewater effluent. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 37(3): 788-796, (2018).