,The horn fly, Haematobia irritans irritans (Linnaeus, 1758; Diptera: Muscidae), a hematophagous external parasite of cattle, causes considerable economic losses to the livestock industry worldwide. This pest is mainly controlled with insecticides; however, horn fly populations from several countries have developed resistance to many of the products available for their control. In an attempt to better understand the adult horn fly and the development of resistance in natural populations, we used an Illumina paired-end read HiSeq and GAII approach to determine the transcriptomes of untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males from a Louisiana population of horn flies with a moderate level of pyrethroid resistance. A total of 128,769,829, 127,276,458, 67,653,920, and 64,270,124 quality-filtered Illumina reads were obtained for untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males, respectively. The de novo assemblies using CLC Genomics Workbench 8.0.1 yielded 15,699, 11,961, 2672, 7278 contigs (≥ 200 nt) for untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males, respectively. More than 56% of the assembled contigs of each data set had significant hits in the BlastX (UniProtKB/Swiss-Prot database) (E <0.001). The number of contigs in each data set with InterProScan, GO mapping, Enzyme codes and KEGG pathway annotations were: Untreated Control Adult Females – 10,331, 8770, 2963, 2183; Untreated control adult males – 8392, 7056, 2449, 1765; Permethrin-treated surviving adult males – 1992, 1609, 641, 495; Permethrin + PBO-treated killed adult males – 5561, 4463, 1628, 1211.,Data is with this article and also available at the National Center for Biotechnology Information (NCBI) Short Read Archive (SRA) through the direct link https://www.ncbi.nlm.nih.gov/sra/SRP131897 or through SRA accession number SRP131897. The adult horn fly transcriptome Shotgun Assembly project has been deposited at DDBJ/EMBL/GenBank under the accession GGLM00000000. The version described in this paper is the first version, GGLM01000000. The overall BioProject ID is PRJNA429442 and the BioSample accessions are SAMN08355023, SAMN08355024, SAMN08355025, and SAMN08355026.,,

,Expressed genes from two individual sibling specimens of Coleomegilla maculata (Coleoptera: Coccinellidae). One individual was fed only insect eggs as an adult, and one was fed only pollen as an adult. Two sequenced samples, total RNA from a single individual adult specimen of Coleomegilla maculata, a beneficial lady beetle common in agroecosystems and native to North America. One sample was an adult fed only insect eggs (carnivore diet) and one sample was an adult fed only pollen (plant-based diet); insects were reared from the same egg mass (siblings), fed identical diet while in larval stage.,BioProject: Coleomegilla maculata strain:inbred Mississippi Transcriptome or Gene expression Taxonomy: Coleomegilla maculata Project data type: Transcriptome or Gene expression Scope: Multiisolate US Department of Agriculture Accession: PRJNA236444 ID: 236444,,

,Reproductive output and behavioral data from stored-product pest insects exposed to long-lasting insecticide treated netting. Includes offspring output and survival for Trogoderma variabile (treated as larvae) and Tribolium castaneum (treated as adults).,,

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,This package includes the data from field experiments to measure the range of attraction of two "male lures" on two different pest fruit fly species via Mark-Release-Recapture (MRR). These values will be of importance to those seeking to optimize fruit fly detection networks or other networks of traps. Methyl eugenol is found to be more attractive to Bactrocera dorsalis compared with trimedlure to Ceratitis capitata. Data consists of number released, proportion responsive, quality control assay results, and recaptures in traps set in a grid pattern after the release.,Resources in this dataset:,,

,Background: The Old-World sand fly, Phlebotomus papatasi (Scopoli), a vector of zoonotic cutaneous leishmaniasis, is usually controlled by insecticides, including anticholinesterases. Previous studies revealed 85% amino acid sequence identity of recombinant P. papatasi acetylcholinesterase (rPpAChE1) to mosquito AChE and identified synthetic carbamates that selectively inhibited rPpAChE1 and circumvented the G119S mutation responsible for high level resistance to anticholinesterases. This study reports the construction, baculovirus expression, and biochemical properties of rPpAChE1 containing the F290V and F331W orthologous mutations from mosquitoes.,Methods: Recombinant PpAChE1 enzymes with or without the F290V, F331W, and G119S orthologous mosquito mutations were expressed in Sf21cells utilizing the baculoviral system. Ellman assays determined changes in catalytic properties and inhibitor sensitivity resulting from wild type and mutant rPpAChE1 containing single or combinations of orthologous mosquito mutations.,Results: Each of the orthologous mutations (F290V, F331W, and G119S) from mosquito AChE significantly reduced inhibition sensitivity to organophosphate or carbamate pesticides, and catalytic activity was lost when they were expressed in combination. Novel synthetic carbamates were identified that significantly inhibited the rPpAChEs expressing each of the single orthologous mosquito mutations.,Conclusions: These novel carbamates could be developed as efficacious insecticides with improved specificity and safety for use in sand fly or mosquito populations expressing the mutant AChEs.,