Data set to support the manuscript "Increased endocrine activity of xenobiotic chemicals as mediated by metabolic activation". Contains analytical measurement of metabolites, binding potential to the estrogen receptor, and gene activation to vitellogenin induction in the rainbow trout assays. Chemicals of interest in this study were phenolphthalein, phenolphthalin, and 4,4’-methylenedianiline. This dataset is associated with the following publication: Kolanczyk, R., J. Denny, B. Sheedy, V. Olson, J. Serrano, and M. Tapper. Increased Endocrine Activity of Xenobiotic Chemicals as Mediated by Metabolic Activation.. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 42(12): 2747-2757, (2023).

Files contain summary reports of chemical and Mass Spectrometry raw and analyzed data used as basis for plot generation and text discussions in the Cyclic phenone metabolism manuscript. File dataset includes a Definition Xcel file listing the supporting files in set. Specifically, the dataset includes individual chemical slice exposure Xcel data summaries for the model cyclic phenones DPK, CBP and CPK as well as well as hepatocyte cytosol exposure data to the same chemicals. Each file contains data organized in labelled tabs that includes General Experimental information, Quantitative and qualitative methods used to process/analyze the raw data for parent and chemical metabolites, chemical mass balances and conclusions. Power point files with the summarized data were presented in the manuscript's Supplemental data. This dataset is associated with the following publication: Serrano, J., M. Tapper, R. Kolanczyk, B. Sheedy, T. Lahren, D. Hammermeister, J. Denny, M. Hornung, A. Kubatova, P. Kosian, J. Voelker, and P. Schmieder. Metabolism of cyclic phenones in rainbow trout in vitro assays. XENOBIOTICA. Taylor & Francis, Inc., Philadelphia, PA, USA, 50(2): 115-131, (2019).

,All-female lines are preferred for rainbow trout production because early sexual maturation in males causes reductions in production efficiency and product quality. However, spontaneous males were reported in genetically all-female XX populations. The objective of this study was to identify single nucleotide polymorphisms (SNPs) associated with spontaneous sex reversal to males in XX rainbow trout (sXX sex reversal). Two families, ST15 and ST22, were genotyped with the Axiom 57K SNP array, and 55 significant SNPs associated with sXX sex reversal were identified and 53 out of the 55 SNPs were located on chromosome OmyA26.,The genotypes of families ST15 and ST22 are included in this dataset. The chromosome numbers and SNPs locations are based on the Arlee reference genome. SNPs cannot be mapped to the genome were assigned to chromosome 34.,

Dataset consists of 2 main files as follows: 1. All main and Supplemental MS final figures used for manuscript preparation for better viewing (pdf file). Figures are copies of raw or analyzed data directly from the mass spectrometers experimental files generated during research. Explanation of figures is provided in manuscript's text 2. Xcel file (Mass calculator CPK metabolites original), containing a significant amount of MS raw data obtained during the research for parent chemicals and metabolites. Emphasis was provided to the analyses of CPK metabolites. The file contains an Index page stating the content of each sheet. Because CPK metabolites were labelled differently as the research progressed, a Legend with the different labeling of each metabolite is presented on each sheet. This dataset is associated with the following publication: Kolanczyk, R., J. Serrano, M. Tapper, and P. Schmieder. A comparison of fish pesticide metabolic pathways with those of the rat and goat. REGULATORY TOXICOLOGY AND PHARMACOLOGY. Elsevier Science Ltd, New York, NY, USA, 94: 124-143, (2018).

These data were compiled to describe population reproductive structure of rainbow trout in the Colorado River downstream of Glen Canyon Dam, Arizona. Objective(s) of our study were to describe the population on reproductive structure of the rainbow trout population in Glen Canyon on the Colorado River and evaluate the accuracy of nonlethal methods (manual expression and ultrasonography) for assigning sex and reproductive phase. These data represent samples collected in 3 sampling trips fall in October to November 2018, February 2019, and April 2019. These data were collected in Glen Canyon, from Glen Canyon Dam to Lees Ferry on the Colorado River. Specifically, we sampled three reaches within Glen Canyon to represent the upper (A: 2.0 - 4.8 km downstream of the dam), middle (B: 8.7 – 11.2 km), and lower (C: 18 – 20.7 km) sections of the tailwater, representing a little more than 1/3rd of the 25-km length of Glen Canyon. These data were collected by the study authors (Crossman, Webb, and Korman) as part of ongoing USGS Trout Recruitment and Growth Dynamics sampling trips in 2018 and 2019. Rainbow trout were sampled from each sampling site on each trip and approximately equal numbers taken from each of four size classes (100-199, 200-299, 300-399, 400-499 mm fork length). Fish were measured for length and weighed and a piece of gonad tissue was preserved for histological assignment in the lab. These data can be used to describe population reproductive structure of rainbow trout, understand less invasive methods for assigning sex, and improve our understanding of how somatic growth and energetic status influence population reproductive structure, reproductive seasonality, and later recruitments.

"Morshead ML, Jensen KM, Ankley GT, Vliet S, LaLone CA, Aller AV, Watanabe KH, Villeneuve DL. Putative adverse outcome pathway development based on physiological responses of female fathead minnows to model estrogen versus androgen receptor agonists. Aquat Toxicol. 2023 Aug;261:106607. doi: 10.1016/j.aquatox.2023.106607. Epub 2023 Jun 9. PMID: 37354817.". This dataset is associated with the following publication: Morshead, M., K. Jensen, G. Ankley, S. Vliet, C. LaLone, D. Villeneuve, A. Vidales Aller, and K. Watanabe. Putative adverse outcome pathway development based on physiological responses of female fathead minnows to model estrogen versus androgen receptor agonists. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 261: 106607, (2023).

Field estimates of the abundance of two trout species (bull trout and westslope cutthroat trout) in Montana and rainbow trout in Washington and British Columbia were collected in concert with environmental DNA samples (eDNA) to evaluate if eDNA copy numbers correlated with abundance of trout. In addition, stream habitat data including channel units (pools, riffles), substrate, large woody debris, among others, were collected at sites.

We investigated whether the length of time fire chemical weathers on a terrestrial substrate before mixing into aquatic environments alters the chemical’s toxicity when encountered by juvenile rainbow trout. We also looked at whether the type of substrate where the chemical is applied affects its toxicity to juvenile rainbow trout after mixing into water. Two fire chemicals (Phos Chek LC95A-R and Phos Chek MVP-Fx) were applied to four substrate media (low organic content soil, high organic content soil, duff, and gravel) and allowed to age on the substrate for 7, 14, 28 or 56 days. At the end of the assigned weathering period, 96-hour assays were conducted by adding water to the dosed substrate and stocking each experimental unit with juvenile rainbow trout. The fish were added to the experimental units after allowing the substrate to settle for 60 minutes. Water chemistry parameters were measured at the initiation of each assay, including water temperature, dissolved oxygen, specific conductance, pH, alkalinity, hardness, and total ammonia. Observations on mortality of rainbow trout were made daily and dead fish were removed upon discovery. The experiment concluded 96 hours after fish entered the experiment.

We investigated whether the length of time fire chemical weathers on a terrestrial substrate before mixing into aquatic environments alters the chemical’s toxicity when encountered by juvenile rainbow trout. We also looked at whether the type of substrate where the chemical is applied affects its toxicity to juvenile rainbow trout after mixing into water. Two fire chemicals (Phos Chek LC95A-R and Phos Chek MVP-Fx) were applied to four substrate media (low organic content soil, high organic content soil, duff, and gravel) and allowed to age on the substrate for 7, 14, 28 or 56 days. At the end of the assigned weathering period, 96-hour assays were conducted by adding water to the dosed substrate and stocking each experimental unit with juvenile rainbow trout. The fish were added to the experimental units after allowing the substrate to settle for 60 minutes. Water chemistry parameters were measured at the initiation of each assay, including water temperature, dissolved oxygen, specific conductance, pH, alkalinity, hardness, and total ammonia. Observations on mortality of rainbow trout were made daily and dead fish were removed upon discovery. The experiment concluded 96 hours after fish entered the experiment.