수인성식품매개감염병 발생의 원인규명 및 전파차단을 위해 감염환자로 부터 원인병원체를 확인하고 유행 추이를 분석하는 사업 관련 데이터입니다.

,Recent USDA/ARS patent- and PVP-protected plant cultivars that are available for licensing are described, including summary, contact, and patent number/status. Updated June 2018.,,

Deformed wing virus (DWV) is a major pathogen of concern to apiculture, and recent reports have indicated the local predominance and potential virulence of recombinants between DWV and a related virus, Varroa destructor virus 1 (VDV). However, little is known about the frequency and titer of VDV and recombinants relative to DWV generally. In this study, I assessed the relative occurrence and titer of DWV and VDV in public RNA-seq accessions of honey bee using a rapid, kmer-based approach. Three recombinant types were detectable graphically and corroborated by de novo assembly. Recombination breakpoints did not disrupt the capsid-encoding region, consistent with previous reports, and both VDV- and DWV-derived capsids were observed in recombinant backgrounds. High abundance of VDV kmers was largely restricted to recombinant forms. Non-metric multidimensional scaling identified genotypic clusters among DWV isolates, which was corroborated by read mapping and consensus generation. The recently described DWV-C lineage was not detected in the searched accessions. The data further highlight the utility of high-throughput sequencing to monitor viral polymorphisms and statistically test biological predictors of titer, and point to the need for consistent methodologies and sampling schemes.

A survey of public honey bee sequence data was performed to detect infections by Lake Sinai Virus (LSV). The Sequence Read Archive of the National Center for Biotechnology Information (NCBI) was queried to identify accessions of RNA sequence data derived from honey bee. These were filtered as described below and then up to 50 million reads or read pairs were downloaded and searched against a reference database of conserved LSV sequence. Accessions with matches above a specified threshold were downloaded in their entirety and assembled into longer contiguous sequences (contigs). The result contigs were searched against each open reading frame (ORF) of the reference LSV genome present in the NCBI database (accession NC_032433.1) and matching regions from each contig. These ORF sequences were aligned with additional sequences identified in NCBI databases through the BLAST web service. These alignments provide the basis for computing phylogenetic trees, rates of nucleotide substitution, codon usage bias, and other evolutionary parameters.

These data describe the results of virus isolation from oropharyngeal/cloacal swabs and testing of sera by a commercial blocking enzyme-linked immunosorbent assay (bELISA) and hemagglutinin (HA) specific testing by microneutralization (MN) and hemagglutination inhibition (HI) for Lesser Scaup in both the Atlantic and Pacific Flyways and Greater Scaup in the Pacific Flyway. These data support a USGS published manuscript.

These data describe the results of virus isolation from oropharyngeal/cloacal swabs and testing of sera by a commercial blocking enzyme-linked immunosorbent assay (bELISA) and hemagglutinin (HA) specific testing by microneutralization (MN) and hemagglutination inhibition (HI) for Lesser Scaup in both the Atlantic and Pacific Flyways and Greater Scaup in the Pacific Flyway. These data support a USGS published manuscript.

Three datasets are included: 1) survival of domesticated canaries and American crows following sub-cutaneous challenges ranging from 101 – 105 plaque forming units of West Nile virus. 2) Arbitrary units of WNV detected by RT-PCR or plaque forming units of WNV cultured in vero cells in 4 separate studies. Culture results are indicated for each day post WNV challenge. 3) Weight (mass) changes in grams in canaries and crows each day following WNV challenge. Day 0 (inoculation) set to 0 gms, then each subsequent day is change in gms from previous day.

Three datasets are included: 1) survival of domesticated canaries and American crows following sub-cutaneous challenges ranging from 101 – 105 plaque forming units of West Nile virus. 2) Arbitrary units of WNV detected by RT-PCR or plaque forming units of WNV cultured in vero cells in 4 separate studies. Culture results are indicated for each day post WNV challenge. 3) Weight (mass) changes in grams in canaries and crows each day following WNV challenge. Day 0 (inoculation) set to 0 gms, then each subsequent day is change in gms from previous day.