Dry thermal treatment of soil contaminated with B. anthracis surrogate spores; study efficacy data from heat chamber and soil column tests

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The data are from the oven tests and the soil column tests in seven MS Excel spreadsheets. Included are the raw plate and ancillary data used to calculate the number for spores recovered from each soil sample, in colony forming units (CFU). This dataset is associated with the following publication: Wood, J., A. Touati, A. Abdel-Hady, D. Aslett, F. Delafield, W. Calfee, E. Silvestri, S. Serre, L. Mickelsen, C. Tomlinson, and A. Mikelonis. Decontamination of soil contaminated at the surface with Bacillus anthracis spores using dry thermal treatment. JOURNAL OF ENVIRONMENTAL MANAGEMENT. Elsevier Science Ltd, New York, NY, USA, 10, (2020).

Efficacy data for inactivation of Bacillus anthracis (anthrax) spores in soil using formaldehyde solutions

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These are the efficacy data for each test conducted in study. Data are presented for Bacillus atrophaeus and B. anthracis in colony forming units, for positive controls and for CFU recovered after decontamination. Log reduction calculations are also included, as well as data on temperature and relative humidity. This dataset is associated with the following publication: Richter, W., M. Sunderman, Z. Willenberg, M. Calfee, S. Serre, and J. Wood. Effectiveness of Formaldehyde in Various Soil Types as a Wide Area Decontamination Approach for Bacillus anthracis Spores. PLOS ONE. Public Library of Science, San Francisco, CA, USA, e0277941, (2022).

Processing Difficult B. anthracis Sample Matrices 6-2022 Manuscript Submission Dataset

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Dataset for bench-scale project evaluating methods to improve detection and sensitivity of Bacillus anthracis detection in 'difficult' sample matrices (i.e., soil, wet vacuum, stormwater runoff). This dataset is associated with the following publication: Nelson, S., K. Hofacre, S. Shah, E. Silvestri, V. Gallardo, A. Mikelonis, R. James, and M. Calfee. Evaluation of Sample Processing Methods to Improve Detection of Bacillus anthracis in Difficult Sample Matrices. ENVIRONMENTAL MONITORING AND ASSESSMENT. Springer, New York, NY, USA, 194(10): 789, (2022).

Data for decontamination of soil using steam

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The data are for the four phases of soil column testing, and include the colony forming unit (CFU) results and the steam and temperature data. Refer to the data dictionary for more details. This dataset is associated with the following publication: Wood, J., T. Chamberlain, A. Touati, D. Aslett, A. Abdel-Hady, M. Monge, W. Calfee, A. Mikelonis, E. Silvestri, S. Serre, and C. Hintz. Decontamination of soil contaminated at the surface with Bacillus anthracis (anthrax) surrogate spores using steam vapor. ENVIRONMENTAL ENGINEERING SCIENCE. Mary Ann Liebert, Inc., Larchmont, NY, USA, 42(2): 92-102, (2025).

Dataset - Evaluation of Standardized Sample Collection, Packaging, and Decontamination Procedures to Assess Cross-Contamination Potential during Bacillus anthracis Incident Response Operations

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Spore recovery data during sample packaging decontamination tests. This dataset is associated with the following publication: Calfee, W., J. Tufts, K. Meyer, K. McConkey, L. Mickelsen, L. Rose, C. Dowell, L. Delaney, A. Weber, S. Morse, J. Chaitram, and M. Gray. Evaluation of standardized sample collection, packaging, and decontamination procedures to assess cross-contamination potential during Bacillus anthracis incident response operations. JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL HYGIENE. Taylor & Francis, Inc., Philadelphia, PA, USA, 13(12): 12, (2016).

Dataset - Evaluation of Standardized Sample Collection, Packaging, and Decontamination Procedures to Assess Cross-Contamination Potential during Bacillus anthracis Incident Response Operations

공공데이터포털

Spore recovery data during sample packaging decontamination tests. This dataset is associated with the following publication: Calfee, W., J. Tufts, K. Meyer, K. McConkey, L. Mickelsen, L. Rose, C. Dowell, L. Delaney, A. Weber, S. Morse, J. Chaitram, and M. Gray. Evaluation of standardized sample collection, packaging, and decontamination procedures to assess cross-contamination potential during Bacillus anthracis incident response operations. JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL HYGIENE. Taylor & Francis, Inc., Philadelphia, PA, USA, 13(12): 12, (2016).

Outdoor Sampling Paper Data Set

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This data set presents surface sampling data and weather information for Bacillus atrophaeus spores, a surrogate for B. anthracis, from a 210-day outdoor study that evaluated the detection and recovery of spores using five different sampling methods: sponge sticks, 37 mm vacuum filter cassettes, residential wet vacuums, robotic floor cleaners, and grab samples of soil, leaves, and grass. This dataset is associated with the following publication: Mikelonis, A., A. Abdel-Hady, D. Aslett, K. Ratliff, A. Touati, J. Archer, S. Serre, L. Mickelsen, S. Taft, and W. Calfee. Comparison of surface sampling methods for an extended duration outdoor biological contamination study. ENVIRONMENTAL MONITORING AND ASSESSMENT. Springer, New York, NY, USA, 192(455): 13, (2020).

Outdoor Sampling Paper Data Set

공공데이터포털

This data set presents surface sampling data and weather information for Bacillus atrophaeus spores, a surrogate for B. anthracis, from a 210-day outdoor study that evaluated the detection and recovery of spores using five different sampling methods: sponge sticks, 37 mm vacuum filter cassettes, residential wet vacuums, robotic floor cleaners, and grab samples of soil, leaves, and grass. This dataset is associated with the following publication: Mikelonis, A., A. Abdel-Hady, D. Aslett, K. Ratliff, A. Touati, J. Archer, S. Serre, L. Mickelsen, S. Taft, and W. Calfee. Comparison of surface sampling methods for an extended duration outdoor biological contamination study. ENVIRONMENTAL MONITORING AND ASSESSMENT. Springer, New York, NY, USA, 192(455): 13, (2020).

Outdoor Sampling Paper Data Set

공공데이터포털

This data set presents surface sampling data and weather information for Bacillus atrophaeus spores, a surrogate for B. anthracis, from a 210-day outdoor study that evaluated the detection and recovery of spores using five different sampling methods: sponge sticks, 37 mm vacuum filter cassettes, residential wet vacuums, robotic floor cleaners, and grab samples of soil, leaves, and grass. This dataset is associated with the following publication: Mikelonis, A., A. Abdel-Hady, D. Aslett, K. Ratliff, A. Touati, J. Archer, S. Serre, L. Mickelsen, S. Taft, and W. Calfee. Comparison of surface sampling methods for an extended duration outdoor biological contamination study. ENVIRONMENTAL MONITORING AND ASSESSMENT. Springer, New York, NY, USA, 192(455): 13, (2020).

Inactivation Data.xlsx

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The data set is a spreadsheet that contains results of inactivation experiments that were conducted to to determine the effectiveness of chlorine in inactivating B. anthracis spore surrogates in wash waters similar to waters that would be generated during building decontamination activities. This dataset is associated with the following publication: Gallardo, V., D. Schupp, J. Heckman, R. Krishnan, and E. Rice. Inactivation of Bacillus Spores in Wash Waters Using Dilute Chlorine Bleach Solutions at Different Temperatures and pH Levels. WATER ENVIRONMENT RESEARCH. Water Environment Federation, Alexandria, VA, USA, 1-36, (2017).