A manuscript describing the independent roles of beta adrenergic and glucocorticoid receptors in mediating ozone-induced pulmonary and systemic effects. This dataset is associated with the following publication: Henriquez, A., S. Snow, M.C. Schladweiler, C. Miller, and U. Kodavanti. Independent roles of beta-adrenergic and glucocorticoid receptors in systemic and pulmonary effects of ozone. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 32(4): 155-169, (2020).

This data set pertains to the manuscript "Exacerbation of ozone-induced pulmonary and systemic effects by 2-adrenergic and/or glucocorticoid agonist/s". It shows the raw data for each figure in the manuscript that is created with these data. Basically examining the influence of beta adrenergic and glucocorticoid receptor agonists on ozone-induced lung injury and inflammation. This dataset is associated with the following publication: Henriquez, A., S. Snow, M. Schladweiler, C. Miller, J. Dye, A. Ledbetter, M. Hargrove, U. Kodavanti, and J. Richards. Exacerbation of ozone-induced pulmonary and systemic effects by beta2-adrenergic and/or glucocorticoid agonist/s. Scientific Reports. Nature Publishing Group, London, UK, 9(1): 17925, (2019).

This data set pertains to the manuscript "Exacerbation of ozone-induced pulmonary and systemic effects by 2-adrenergic and/or glucocorticoid agonist/s". It shows the raw data for each figure in the manuscript that is created with these data. Basically examining the influence of beta adrenergic and glucocorticoid receptor agonists on ozone-induced lung injury and inflammation. This dataset is associated with the following publication: Henriquez, A., S. Snow, M. Schladweiler, C. Miller, J. Dye, A. Ledbetter, M. Hargrove, U. Kodavanti, and J. Richards. Exacerbation of ozone-induced pulmonary and systemic effects by beta2-adrenergic and/or glucocorticoid agonist/s. Scientific Reports. Nature Publishing Group, London, UK, 9(1): 17925, (2019).

We have previously shown that neuroendocrine activation leading to increased circulating stress hormones was necessary for mediating ozone-induced lung injury and inflammation since adrenalectomy rats were protected from these ozone effects. Because adrenalectomy is invasive and also eliminates circulating mineralocorticoids along with stress hormones, one cannot rule out their contribution in diminution of ozone-induced lung effects. The goal of this study was to evaluate if agonists of stress hormone receptors β2 adrenergic receptors and glucocorticoid receptors were able to restore ozone-induced lung injury, inflammation and innate immune cell trafficking in adrenalectomy rats, and exacerbate these effects in control rats with sham surgery. Here, we reconfirmed that the pulmonary and systemic effects of ozone inhalation, characterized by vascular leakage, neutrophilic inflammation, cytokine release in the lungs and peripheral vascular lymphopenia, were significantly diminished by adrenlectomy. The treatment with a combination of β2 adrenergic receptor and glucocorticoid receptor agonists (Clenbuterol and dexamethasone) was able to restore these ozone effects in AD rats, and further exacerbate ozone-induced lung protein leakage, inflammation and lymphopenia in sham surgery rats. It was also noted that clenbuterol plus dexamethasone itself caused injury and cytokine increases in the lung. Although a variety of β2 adrenergic receptor and glucocorticoid agonists have been widely used for the treatment of chronic lung diseases, β2 adrenergic receptor agonists have been shown to exacerbate lung inflammation in asthmatics and epidemiological studies have indicated exacerbation of lung inflammation in asthmatics during increased air pollution episodes. Even though high concentrations of agonists were used, our study provides a potential causal mechanistic link between activation of stress hormone receptors in mediation of air pollution health effects, and how these effects might be exacerbated in those receiving asthma therapy. This dataset is associated with the following publication: Henriquez, A., S. Snow, M. Schladweiler, C. Miller, J. Dye, A. Ledbetter, J. Richards, M. Hargrove, W. Williams, and U. Kodavanti. Beta-2 adrenergic and glucocorticoid receptor agonists modulate ozone-induced pulmonary protein leakage and inflammation in healthy and adrenalectomized rats. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 166(2): 288-305, (2018).

This data set contains one Excel file. In this file are all the data pertaining to the effects of propranolol and mifepristone on ozone induced lung injury and inflammation . The different tabs of the spreadsheet pertain to each figure found in the manuscript. This dataset is associated with the following publication: Henriquez, A., S. Snow, M. Schladweiler, C. Miller, J. Dye, A. Ledbetter, J. Richards, K. Mauge-Lewis, M. McGee, and U. Kodavanti. Adrenergic and glucocorticoid receptor antagonists reduce ozone-induced lung injury and inflammation. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 339: 161-171, (2018).

This data set contains one Excel file. In this file are all the data pertaining to the effects of propranolol and mifepristone on ozone induced lung injury and inflammation . The different tabs of the spreadsheet pertain to each figure found in the manuscript. This dataset is associated with the following publication: Henriquez, A., S. Snow, M. Schladweiler, C. Miller, J. Dye, A. Ledbetter, J. Richards, K. Mauge-Lewis, M. McGee, and U. Kodavanti. Adrenergic and glucocorticoid receptor antagonists reduce ozone-induced lung injury and inflammation. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 339: 161-171, (2018).

This data set is an excel file pertaining to the study that examined ozone-induced systemic and pulmonary effects in rats that underwent SHAM surgery (control), adrenal demedullation or total bilateral adrenalectomy. Different pages of the spreadsheet shows individual animal data for markers of lung injury and inflammation, body weights, whole body plethysmography measurements, levels of circulating hormones and lipids, and circulating white blood cell count as well as platelet count. This dataset is associated with the following publication: Miller, D., S. Snow, M. Schladweiler , J. Richards , A. Ghio , A. Ledbetter , and U. Kodavanti. Acute Ozone-Induced Pulmonary and Systemic Metabolic Effects are Diminished in Adrenalectomized Rats#. TOXICOLOGICAL SCIENCES. Society of Toxicology, 150(2): 312-22, (2016).

This data set is an excel file pertaining to the study that examined ozone-induced systemic and pulmonary effects in rats that underwent SHAM surgery (control), adrenal demedullation or total bilateral adrenalectomy. Different pages of the spreadsheet shows individual animal data for markers of lung injury and inflammation, body weights, whole body plethysmography measurements, levels of circulating hormones and lipids, and circulating white blood cell count as well as platelet count. This dataset is associated with the following publication: Miller, D., S. Snow, M. Schladweiler , J. Richards , A. Ghio , A. Ledbetter , and U. Kodavanti. Acute Ozone-Induced Pulmonary and Systemic Metabolic Effects are Diminished in Adrenalectomized Rats#. TOXICOLOGICAL SCIENCES. Society of Toxicology, 150(2): 312-22, (2016).

This data set shows high throughput gene expression assessment using RNAseq to examine how ozone-induced transcriptional changes in the lung are influenced by adrenalectomy or adrenal demedullation in rats. We have previously observed that lung injury and inflammation are diminished in adrenalectomized rats and this study was planned to understand if circulating stress hormones influence ozone transcriptional effects and what ozone-induced pathway changes might be impacted by removal of adrenal glands. This dataset is associated with the following publication: Henriquez, A., J. House, D. Miller, S. Snow, A. Fisher, H. Ren, M. Schladweiler, A. Ledbetter, F. Wright, and U. Kodavanti. Adrenal-derived stress hormones modulate ozone-induced lung injury and inflammation. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 329: 249-258, (2017).

This data set shows high throughput gene expression assessment using RNAseq to examine how ozone-induced transcriptional changes in the lung are influenced by adrenalectomy or adrenal demedullation in rats. We have previously observed that lung injury and inflammation are diminished in adrenalectomized rats and this study was planned to understand if circulating stress hormones influence ozone transcriptional effects and what ozone-induced pathway changes might be impacted by removal of adrenal glands. This dataset is associated with the following publication: Henriquez, A., J. House, D. Miller, S. Snow, A. Fisher, H. Ren, M. Schladweiler, A. Ledbetter, F. Wright, and U. Kodavanti. Adrenal-derived stress hormones modulate ozone-induced lung injury and inflammation. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 329: 249-258, (2017).