Dataset consists of morphometric measurements from unionid Pondmussel (Ligumia subrostrata) and concentrations of four per- and polyfluoroalkyl substances (PFAS) in water and freshwater mussels collected from a 14-day accumulation and 7-day elimination study. The accumulation of four per- and polyfluoroalkyl substances (PFAS) were measured. Samples (4 replicates) were collected at 0, 4, 12, 48, 96, 168, and 336 hours during the uptake phase of the exposure. Samples (4 replicates) were collected at time point 348, 360, 408, and 504 hours during the elimination phase of the exposure. Note the time points are sequential with the uptake portion of the study followed by the elimination portion of the study.

The data consists of targeted metabolite analysis (using quadrupole time-of-flight mass spectroscopy ;UHPLC-QTOF-MS) of hemolymph from two freshwater mussel species (Lampsilis cardium and L. siliquoidea) from three streams in Indiana. Concentrations of metabolites were modeled by species, stream and sex to determine the influence of each parameter.

The data consists of targeted metabolite analysis (using quadrupole time-of-flight mass spectroscopy ;UHPLC-QTOF-MS) of hemolymph from two freshwater mussel species (Lampsilis cardium and L. siliquoidea) from three streams in Indiana. Concentrations of metabolites were modeled by species, stream and sex to determine the influence of each parameter.

Invasion of North American waters by Dreissena polymorpha and D. rostriformis bugensis has resulted in declines in native North American Unionoida mussels. Dreissenid mussels biofoul unionid mussels in large numbers and interfere with unionid movement, acquisition of food and ability to open and close their shells. Initial expectations for the Great Lakes were that unionids would be extirpated where they co-occur with dreissenids, but recently adult and juvenile unionids have been found alive in several apparent refugia. These unionid populations may persist due to reduced dreissenid biofouling in these areas, and/or due to processes that remove biofoulers. For example, locations inaccessible to veligers may reduce biofouling and habitats with soft substrates may allow unionids to burrow and thus remove dreissenids. Here, biofouling was measured by deploying caged unionid mussels (Lampsilis siliquoidea) at 36 sites across the western basin of Lake Erie to assess spatial variation in biofouling and to identify other areas that might promote the persistence or recovery of native unionid mussels. Biofouling ranged from 0.03 – 26.33 g per mussel, reached a maximum in the immediate vicinity of the Maumee rivermouth, and appeared to primarily consist of dreissenid mussels. A known mussel refugium in the vicinity of a power plant near the Maumee rivermouth actually exhibited very high biofouling rates, suggesting low dreissenid colonization is unlikely to be the primary cause of unionid survival in this refugium. The southern nearshore area of Lake Erie, near another refugium, also had very low biofouling. A large stretch of the western basin appeared to have low biofouling rates and muddy substrates, raising the possibility that these open water areas could support remnant and returning populations of unionid mussels.

These datasets provide geographic sampling information for Bathymodiolin mussel samples collected from three cold seep sites off the mid-Atlantic U.S. coast, northwestern Atlantic Ocean. Also included are GenBank accession numbers for mitochondrial gene sequences (COI and ND4) used to identify the mussel species and GenBank accession numbers for BioProject containing 16S metabarcoding sequence data used to identify gill symbionts in mussels.

These datasets provide geographic sampling information for Bathymodiolin mussel samples collected from three cold seep sites off the mid-Atlantic U.S. coast, northwestern Atlantic Ocean. Also included are GenBank accession numbers for mitochondrial gene sequences (COI and ND4) used to identify the mussel species and GenBank accession numbers for BioProject containing 16S metabarcoding sequence data used to identify gill symbionts in mussels.

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

This data set contains toxicity data from 28- and 84-day sediment bioassays to assess the effects of contaminated Kanawha River, West Virginia sediment on juvenile mussels (fatmucket, Lampsilis siliquoidea) and one commonly tested benthic invertebrate (amphipod, Hyalella azteca) following ASTM standard methods for conducting 4-week sediment toxicity tests with the mussel and amphipod. In addition, a longer-term (12-week) sediment test with the mussel was conducted to determine toxic effects in a potentially more sensitive bioassay with a longer test duration. A 28-day sediment dilution bioassay of test site 2 from the initial sediment exposures was also conducted to further evaluate the toxicity.

The data release includes data from four studies: (1) toxicity of a permitted effluent, which entered the Deep Fork River (DFR), Oklahoma, USA, to a unionid mussel (Lampsilis siliquoidea) and to 2 standard test species (cladoceran Ceriodaphnia dubia; and fathead minnow Pimephales promelas) in short-term 7-d effluent tests; (2) relative sensitivities of the 3 species to potassium (K), an elevated major ion in the effluent, using 7-d toxicity tests with KCl spiked into a DFR upstream reference water; (3) potential influences of background water characteristics on the acute K toxicity to the mussel (96-h exposures) and cladoceran (48-h exposure) in 4 reconstituted waters that mimicked the hardness and ionic composition ranges of the DFR; and (4) potential influence of temperature on acute K toxicity to the mussel. Water quality, survival, growth, and reproduction endpoints are reported.