Monitoring change in genetic diversity in wildlife populations across multiple scales could facilitate prioritization of conservation efforts. We used microsatellite genotypes from 7,080 previously collected genetic samples from across the greater sage-grouse (Centrocercus urophasianus) range to develop a modelling framework for estimating genetic diversity within a recently developed hierarchically nested monitoring framework (clusters). The majority of these genetic samples (n=6560) were used in previous research (Oyler-McCance et al. 2014; Cross et. al 2018; Row et. al. 2018). Genetic diversity values associated with clusters across multiple scales could facilitate the identification of areas with low genetic diversity and inform the potential management or conservation priority and response. We also report the data used to define genetic diversity thresholds of conservation concern and a full reporting of the genetic diversity estimates associated with the evaluated clusters.

Genetic variation is a well-known indicator of population fitness yet is not typically included in monitoring programs for sensitive species. Additionally, most programs monitor populations at one scale, which can lead to potential mismatches with ecological processes critical to species’ conservation. Recently developed methods generating hierarchically nested population units (i.e., clusters of varying scales) for greater sage-grouse (Centrocercus urophasianus) have identified population trend declines across spatiotemporal scales to help managers target areas for conservation. The same clusters used as a proxy for spatial scale can alert managers to local units (i.e., fine-scale) with low genetic diversity relative to regional units (i.e., coarse-scale), further facilitating identification of management targets. We developed a genetic warning system utilizing previously developed hierarchical population units to identify management-relevant areas with low genetic diversity within the greater sage-grouse range. Within this warning system we characterized conservation concern thresholds based on values of genetic diversity for hierarchically nested populations. We developed a spatial data set to display genetic diversity values and conservation concern information from a Genetic Warning System (GWS) for population monitoring of greater sage-grouse, as described in Zimmerman et al. (2022). Here we added the genetic diversity estimates (allelic richness and expected heterozygosity) and GWS information as attributes to the relevant fine-scale (level 2) and coarse-scale (level 13) previously developed hierarchically nested population clusters (O’Donnell et al. 2019, O’Donnell et al. 2022). The GWS incorporates population trend decline watches and warnings from the Targeted Annual Warning System (TAWS) for greater sage-grouse as reported in Coates et al. (2021) to further refine degree of conservation concern.

Genetic variation is a well-known indicator of population fitness yet is not typically included in monitoring programs for sensitive species. Additionally, most programs monitor populations at one scale, which can lead to potential mismatches with ecological processes critical to species’ conservation. Recently developed methods generating hierarchically nested population units (i.e., clusters of varying scales) for greater sage-grouse (Centrocercus urophasianus) have identified population trend declines across spatiotemporal scales to help managers target areas for conservation. The same clusters used as a proxy for spatial scale can alert managers to local units (i.e., fine-scale) with low genetic diversity relative to regional units (i.e., coarse-scale), further facilitating identification of management targets. We developed a genetic warning system utilizing previously developed hierarchical population units to identify management-relevant areas with low genetic diversity within the greater sage-grouse range. Within this warning system we characterized conservation concern thresholds based on values of genetic diversity for hierarchically nested populations. We developed a spatial data set to display genetic diversity values and conservation concern information from a Genetic Warning System (GWS) for population monitoring of greater sage-grouse, as described in Zimmerman et al. (2022). Here we added the genetic diversity estimates (allelic richness and expected heterozygosity) and GWS information as attributes to the relevant fine-scale (level 2) and coarse-scale (level 13) previously developed hierarchically nested population clusters (O’Donnell et al. 2019, O’Donnell et al. 2022). The GWS incorporates population trend decline watches and warnings from the Targeted Annual Warning System (TAWS) for greater sage-grouse as reported in Coates et al. (2021) to further refine degree of conservation concern.

The authors and the Bureau of Land Management (BLM) have expanded the greater sage-grouse (GRSG [also referenced as sage-grouse]; Centrocercus urophasianus) hierarchical population units/clusters to ensure inclusion of the proposed revisions (2025) of the BLM habitat management areas (HMAs) in future management implementation decisions. The authors used a consolidated dataset of all GRSG HMAs provided by the BLM from individual Records of Decision (ROD) and Approved Resource Management Plan Amendments (ARMPA) for GRSG in Oregon and Colorado. In addition, the proposed HMAs from the Greater Sage-Grouse Rangewide Planning Proposed Resource Management Plan Amendment and Final Environmental Impact Statement (EIS) for California, Idaho, Montana, Nevada, North Dakota, South Dakota, Utah, and Wyoming were incorporated (DOI-BLM-WO-2300-2022-0001-RMP-EIS). The Federal Land Policy and Management Act requires that Resource Management Plans (RMPs) for managing public lands be developed and maintained, and the National Environmental Policy Act requires that an environmental impact statement (EIS) be prepared for Federal actions significantly affecting the quality of the human environment. The EIS for the Greater Sage-Grouse RMPAs identified updated HMAs, areas of highest conservation value for the species, based on new habitat use data. A consolidated version of the HMAs were provided to USGS authors. Information about designated HMAs is described in the "Supplemental" section of the metadata file. The authors developed three new datasets that reflect revised GRSG HMA boundaries produced by BLM. The new data include a revised GRSG boundary, cluster level 2 (neighborhood clusters; NC), and cluster level 13 (climate clusters; CC). These revisions include any designations or proposed designations of HMAs falling outside previously published population unit/cluster versions (O’Donnell et al. 2022; https://doi.org/10.5066/P9D1K0LX). Background information on original population units/clusters of GRSG: We produced 13 hierarchically nested cluster levels that reflect the results from developing a hierarchical monitoring framework for GRSG across the western United States. Polygons (clusters) within each cluster level group a population of GRSG leks (sage-grouse breeding grounds) and each level increasingly groups lek clusters from previous levels. We developed the hierarchical clustering approach by identifying biologically relevant population units aimed to use a statistical and repeatable approach and include biologically relevant landscape and habitat characteristics. We desired a framework that was spatially hierarchical, discretized the landscape while capturing connectivity (habitat and movements), and supported management questions at different spatial scales. The spatial variability in the amount and quality of habitat resources can affect local population success and result in different population growth rates among smaller clusters. Equally so, the spatial structure and ecological organization driving scale-dependent systems in a fragmented landscape affects dispersal behavior, suggesting inclusion in population monitoring frameworks. Studies that compare conditions among spatially explicit hierarchical clusters may elucidate the cause of differing growth rates at local scales affected by changes in habitat quality compared to larger scaled processes affecting growth rates, such as regional climate/vegetation communities. Therefore, the use of multiple scales (hierarchical cluster levels) that group demographic data can provide information driving population changes at different spatial scales, thereby providing a tool for population monitoring and adaptive management.

Boundary of the conservation assessment of Greater Sage-grouse and sagebrush habitat conducted by the Western Association of Fish and Wildlife Agencies. The boundary is derived from the pre-settlement distribution of the Sage-grouse.

Boundary of the conservation assessment of Greater Sage-grouse and sagebrush habitat conducted by the Western Association of Fish and Wildlife Agencies. The boundary is derived from the pre-settlement distribution of the Sage-grouse.

We produced 13 hierarchically nested cluster levels that reflect the results from developing a hierarchical monitoring framework for greater sage-grouse across the western United States. Polygons (clusters) within each cluster level group a population of sage-grouse leks (sage-grouse breeding grounds) and each level increasingly groups lek clusters from previous levels. We developed the hierarchical clustering approach by identifying biologically relevant population units aimed to use a statistical and repeatable approach and include biologically relevant landscape and habitat characteristics. We desired a framework that was spatially hierarchical, discretized the landscape while capturing connectivity (habitat and movements), and supported management questions at different spatial scales. The spatial variability in the amount and quality of habitat resources can affect local population success and result in different population growth rates among smaller clusters. Equally so, the spatial structure and ecological organization driving scale-dependent systems in a fragmented landscape affects dispersal behavior, suggesting inclusion in population monitoring frameworks. Studies that compare conditions among spatially explicit hierarchical clusters may elucidate the cause of differing growth rates at local scales affected by changes in habitat quality compared to larger scaled processes affecting growth rates, such as regional climate/vegetation communities. Therefore, the use of multiple scales (hierarchical cluster levels) that group demographic data can provide information driving population changes at different spatial scales, thereby providing a tool for population monitoring and adaptive management.

We produced 13 hierarchically nested cluster levels that reflect the results from developing a hierarchical monitoring framework for greater sage-grouse across the western United States. Polygons (clusters) within each cluster level group a population of sage-grouse leks (sage-grouse breeding grounds) and each level increasingly groups lek clusters from previous levels. We developed the hierarchical clustering approach by identifying biologically relevant population units aimed to use a statistical and repeatable approach and include biologically relevant landscape and habitat characteristics. We desired a framework that was spatially hierarchical, discretized the landscape while capturing connectivity (habitat and movements), and supported management questions at different spatial scales. The spatial variability in the amount and quality of habitat resources can affect local population success and result in different population growth rates among smaller clusters. Equally so, the spatial structure and ecological organization driving scale-dependent systems in a fragmented landscape affects dispersal behavior, suggesting inclusion in population monitoring frameworks. Studies that compare conditions among spatially explicit hierarchical clusters may elucidate the cause of differing growth rates at local scales affected by changes in habitat quality compared to larger scaled processes affecting growth rates, such as regional climate/vegetation communities. Therefore, the use of multiple scales (hierarchical cluster levels) that group demographic data can provide information driving population changes at different spatial scales, thereby providing a tool for population monitoring and adaptive management.

We developed a hierarchical clustering approach that identifies biologically relevant landscape units that can 1) be used as a long-term population monitoring framework, 2) be repeated across the Greater sage-grouse range, 3) be used to track the outcomes of local and regional populations by comparing population changes across scales, and 4) be used to inform where to best spatially target studies that identify the processes and mechanisms causing population trends to change among spatial scales. The spatial variability in the amount and quality of habitat resources can affect local population success and result in different population growth rates among smaller clusters. Equally so, the spatial structure and ecological organization driving scale-dependent systems in a fragmented landscape affects dispersal behavior, suggesting inclusion in population monitoring frameworks. Studies that compare conditions among spatially explicit hierarchical clusters may elucidate the cause of differing growth rates, indicating the appropriate location and spatial scale of a management action. The data presented here reflect the results from developing a hierarchical monitoring framework and then applying these methods to Greater Sage-grouse in Nevada and Wyoming, US. When using these data for evaluating population changes or when identifying a spatially balanced sampling protocol, all cluster levels are designed to work together and therefore we recommend evaluating multiple cluster levels prior to selecting a single cluster level, if a single scale is desired, when analyzing population growth rates or other analyses, as these data are intended for multi-scale efforts. In other words, let your data decide which scale(s) are appropriate for the given species. These cluster levels are specific to Greater Sage-grouse but they may be appropriate for other sagebrush obligate species, but the user will need to make this determination. The products from this study aim to support multiple research and management needs. However, these data represent an interim data product because there may be errors associated with clusters along the edges of the state boundaries (due to the lack of lek data in neighboring states). We are planning to release new data that we will develop for the Greater sage-grouse range. We recommend using the new data products once available instead of these data products. These data will remain online as they are associated with the following citation, which provides a detailed explanation of the methods used to develop these data: O’Donnell, Michael S., David R. Edmunds, Cameron L. Aldridge, Julie A. Heinrichs, Peter S. Coates, Brian G. Prochazka, and Steve E. Hanser. 2018. Designing hierarchically nested and biologically relevant monitoring frameworks to study populations across scales. Ecosphere

We developed a hierarchical clustering approach that identifies biologically relevant landscape units that can 1) be used as a long-term population monitoring framework, 2) be repeated across the Greater sage-grouse range, 3) be used to track the outcomes of local and regional populations by comparing population changes across scales, and 4) be used to inform where to best spatially target studies that identify the processes and mechanisms causing population trends to change among spatial scales. The spatial variability in the amount and quality of habitat resources can affect local population success and result in different population growth rates among smaller clusters. Equally so, the spatial structure and ecological organization driving scale-dependent systems in a fragmented landscape affects dispersal behavior, suggesting inclusion in population monitoring frameworks. Studies that compare conditions among spatially explicit hierarchical clusters may elucidate the cause of differing growth rates, indicating the appropriate location and spatial scale of a management action. The data presented here reflect the results from developing a hierarchical monitoring framework and then applying these methods to Greater Sage-grouse in Nevada and Wyoming, US. When using these data for evaluating population changes or when identifying a spatially balanced sampling protocol, all cluster levels are designed to work together and therefore we recommend evaluating multiple cluster levels prior to selecting a single cluster level, if a single scale is desired, when analyzing population growth rates or other analyses, as these data are intended for multi-scale efforts. In other words, let your data decide which scale(s) are appropriate for the given species. These cluster levels are specific to Greater Sage-grouse but they may be appropriate for other sagebrush obligate species, but the user will need to make this determination. The products from this study aim to support multiple research and management needs. However, these data represent an interim data product because there may be errors associated with clusters along the edges of the state boundaries (due to the lack of lek data in neighboring states). We are planning to release new data that we will develop for the Greater sage-grouse range. We recommend using the new data products once available instead of these data products. These data will remain online as they are associated with the following citation, which provides a detailed explanation of the methods used to develop these data: O’Donnell, Michael S., David R. Edmunds, Cameron L. Aldridge, Julie A. Heinrichs, Peter S. Coates, Brian G. Prochazka, and Steve E. Hanser. 2018. Designing hierarchically nested and biologically relevant monitoring frameworks to study populations across scales. Ecosphere