This data set includes information on collections of fecal or cloacal samples from wild gulls (Larus spp.) at four locations on the Kenai Peninsula, Alaska, USA. Samples were also collected from sockeye salmon harvested as part of the personal-use fisheries on the Kenai and Kasilof Rivers. Samples were screened for Escherichia coli (E. coli) and tested for resistance to multiple antibiotics.

These data represent ten (10) weeks of monitoring E. coli levels in Abrams Creek upstream and downstream of the Cades Cove Riding Stables in Cades Cove, TN, GRSM. Water samples from Abrams Creek were later taken to Twin Creeks Science Center, Gatlinburg, TN, GRSM, where nutrient packs were added to the samples, which were then aliquoted into sealed trays and placed in an incubator for 24-hours. After the 24-hour incubation period, trays were removed and placed under a blacklight hood to determine the number of aliquoted wells that fluoresced from bacterial growth. From these observations, E.coli numbers were recorded and later quantified using the IDEXX Water MPN Generator V 1.4.4.

,This dataset includes soil health, crop biomass, and crop yield data for a 13-year corn stover harvest trial in central Iowa.,Following the release in 2005 of the Billion Ton Study assessment of biofuel sources, several soil health assessments associated with harvesting corn stover were initiated across ARS locations to help provide industry guidelines for sustainable stover harvest. This dataset is from a trial conducted by the National Laboratory for Agriculture and Environment from 2007-2021 at the Iowa State University Ag Engineering and Agronomy farm. Management factors evaluated in the trial included the following.,The dataset includes: 1) Crop biomass and yields for all crop phases in every year. 2) Soil organic carbon, total carbon, total nitrogen, and pH to 120 cm depth in 2012, 2016, and 2017. Soil cores from 2005 (pre-study) were also sampled to 90 cm depth. 3) Soil chemistry sampled to 15 cm depth every 1-2 years from 2007 to 2017. 4) Soil strength and compaction was assessed to 60 cm depth in April 2021.,These data have been presented in several manuscripts, including Phillips et al. (in review), O'Brien et al. (2020), and Obrycki et al. (2018).,

,REAP Study for Resilient Economic Agricultural Practices in West Lafayette, Indiana Corn stover is an important livestock feed and will probably be a major source of renewable bioenergy, especially in the U.S. Corn Belt. Overly aggressive removal of stover, however, could lead to greater soil erosion and hurt producer yields in the long-run. Good residue management practices could help prevent erosion of valuable topsoil by wind and water while still providing a revenue source for producers, either as livestock feed or for use in renewable bioenergy. Plant residues also contribute to soil structure, nutrient cycling, and help sustain the soil microbiota. Good residue management could also help control the loss of greenhouse gases from agricultural soils that could add to already increasing levels of atmospheric greenhouse gases contributing to global climate change. Cumulative GHG emissions varied widely across locations, by management, and from year-to-year. Despite this high variability, maximum stover removal averaged across all sites, years, and management resulted in lower total emissions of CO2 (-12 ± 11%) and N2O (-13 ± 28%) compared to no stover removal. Decreases in total CO2 and N2O emissions in stover removal treatments were attributed to decreased availability of stover-derived C and N inputs into soils, as well as possible microclimatic differences. Soils at all sites were CH4 neutral or small CH4 sinks. Exceptions to these trends occurred for all GHGs, highlighting the importance of site-specific management and environmental conditions on GHG fluxes in agricultural soils.,

,EV Smith Study for Resilient Economic Agricultural Practices in Auburn, Alabama There is a potential in the southeastern US to harvest winter cover crops from cotton (Gossypium hirsutum L.) fields for biofuels or animal feed use, but this could impact yields and nitrogen (N) fertilizer response. An experiment was established to examine rye (Secale cereale L.) residue management (RM) and N rates on cotton productivity. Three RM treatments (no winter cover crop (NC), residue removed (REM) and residue retained (RET)) and four N rates for cotton were studied. Cotton population, leaf and plant N concentration, cotton biomass and N uptake at first square, and cotton biomass production between first square and cutout were higher for RET, followed by REM and NC. However, leaf N concentration at early bloom and N concentration in the cotton biomass between first square and cutout were higher for NC, followed by REM and RET. Seed cotton yield response to N interacted with year and RM, but yields were greater with RET followed by REM both years. These results indicate that a rye cover crop can be beneficial for cotton, especially during hot and dry years. Long-term studies would be required to completely understand the effect of rye residue harvest on cotton production under conservation tillage.,

,Nutrient cycling is a key ecosystem service provided by soils, which may be impacted by global change-induced droughts and alterations to grazing pressure. While the belowground abiotic and biotic responses to drought are increasingly studied, linkages among plant, animal, and microbial responses to drought remain poorly understood. Here we used an innovative experimental approach to enable understanding the relative importance of rainfall reduction, bovine grazing, and their interplay on soil nutrient pools and processes during and after treatments. Specifically, we experimentally imposed a two-year drought of varying intensity (five levels) at two northern mixed-grass prairie sites in Montana and Wyoming. Crossed with this drought treatment, we also imposed a gradient of bovine grazing pressure during the two drought years and three years of recovery following the drought. We found that rainfall reductions at both sites resulted in reductions in soil available P and micronutrients during treatment application. Conversely, rainfall reductions caused both immediate and persistent increases in soil NO3-. Soil nutrients were generally unaffected by grazing treatments. In contrast, biotic soil properties including the activities of six extracellular enzymes and bacterial and fungal community compositions were relatively resistant to rainfall reduction treatments. However, grazing treatments appeared to have a greater effect on extracellular enzyme activity potentials and soil microbial community composition. Overall, our results highlight the relative stability of belowground processes in semi-arid rangelands in the face of drought and land management strategies.,The article utilizing this dataset is at https://doi.org/10.1016/j.soilbio.2025.110071.,

,2014 Swine CAFO Study SE for Agricultural Antibiotic Resistance in Mississippi State, Mississippi The environmental influence of farm management in concentrated animal feeding operations (CAFO) can yield vast changes to the microbial biota and ecological structure of both the pig and waste manure lagoon wastewater. While some of these changes may not be negative, it is possible that CAFOs can enrich antibiotic resistant bacteria or pathogens based on farm type, thereby influencing the impact imparted by the land application of its respective wastewater. The purpose of this study was to measure the microbial constituents of swine-sow, -nursery, and -finisher farm manure lagoon wastewater and determine the changes induced by farm management. A total of 37 farms were visited in the Mid-South USA and analyzed for the genes 16S rRNA, spaQ (Salmonella spp.), Camp-16S (Campylobacter spp.), tetA, tetB, ermF, ermA, mecA, and intI using quantitative PCR. Additionally, 16S rRNA sequence libraries were created. Overall, it appeared that finisher farms were significantly different from nursery and sow farms in nearly all genes measured and in 16S rRNA clone libraries. Nearly all antibiotic resistance genes were detected in all farms. Interestingly, the mecA resistance gene (e.g. methicillin resistant Staphylococcus aureus) was below detection limits on most farms, and decreased as the pigs aged. Finisher farms generally had fewer antibiotic resistance genes, which corroborated previous phenotypic data; additionally, finisher farms produced a less diverse 16S rRNA sequence library. Comparisons of Camp-16S and spaQ GU (genomic unit) values to previous culture data demonstrated ratios from 10 to 10,000:1 depending on farm type, indicating viable but not cultivatable bacteria were dominant. The current study indicated that swine farm management schemes positively and negatively affect microbial and antibiotic resistant populations in CAFO wastewater which has future “downstream” implications from both an environmental and public health perspective.,

,On-Farm Residue Removal Study for Resilient Economic Agricultural Practices in Morris, Minnesota Interest in harvesting crop residues for energy has waxed and waned since the oil embargo of 1973. Since the at least the late 1990’s interest has been renewed due to concern of peak oil, highly volatile natural gas prices, replacing fossil fuel with renewable sources and a push for energy independence. The studies conducted on harvesting crop residues during the 1970’s and1980’s focused primarily on erosion risk and nutrient removal as a result early estimates of residue availability focused on erosion control (Perlack et al., 2005). More recently, the focus has expanded to also address harvest impacts on soil organic matter and other constraints (Wilhelm et al., 2007; Wilhelm et al., 2010). In West Central Minnesota, crop residues have been proposed a replacement for natural gas (Archer and Johnson, 2012) while nationally residues are also be considered for cellulosic ethanol production (US DOE, 2011). The objective of the on-farm study was to assess the impact of residue harvest on working farms with different management systems and soils. Indicators of erosion risk, soil organic matter, and crop productivity is response to grain plus cob, or grain plus stover compared to grain only harvest.,

,This is digital research metadata corresponding to a published manuscript in Energies (MDPI) entitled "Biochar stability in a highly weathered sandy soil under four years of continuous corn production", Volume 14, Issue 19, 6157. Dataset may be accessed via the included link at the Dryad data repository.,Biochar is being considered a climate change mitigation tool by increasing soil organic carbon contents (SOC), however, questions remain concerning its longevity in soil. We applied 30,000 kg ha−1 of biochars to plots containing a Goldsboro sandy loam (Fine-loamy, siliceous, sub-active, thermic Aquic Paleudults) and then physically disked all plots. Thereafter, the plots were agronomically managed under 4 years (Y) of continuous corn (Zea mays, L.) planting. Annually, incremental soil along with corresponding bulk density samples were collected and SOC concentrations were measured in topsoil (down to 23-cm). The biochars were produced from Lodgepole pine (Pinus contorta) chip (PC) and Poultry litter (PL) feedstocks. An untreated Goldsboro soil (0 biochar) served as a control. After four years, SOC contents in the biochar treated plots were highest in the top 0–5 and 5–10 cm depth suggesting minimal deeper movement. Declines in SOC contents varied with depth and biochar type. After correction for SOC declines in controls, PL biochar treated soil had a similar decline in SOC (7.9 to 10.3%) contents. In contrast, the largest % SOC content decline (20.2%) occurred in 0–5 cm deep topsoil treated with PC biochar. Our results suggest that PC biochar had less stability in the Goldsboro soil than PL biochar after 4 years of corn grain production.,Methods are described in the manuscript: https://doi.org/10.3390/en14196157. Descriptions corresponding to each figure and table in the manuscript are placed on separate tabs in the Excel file to clarify abbreviations and summarize the data headings and units.,,