Solibacillus kalamii was isolated from a HEPA filter in the International Space Station. This strain was of particular interest due to the unique environment in which it was isolated from.

Draft genomes of various bacterial phyla isolated from different locations on the International Space Station, as part of the Microbial Tracking 2 mission (ISS-MOP).

The microbial tracking-1 (MT-1) investigation allowed the characterization of the microbial population aboard the International Space Station (ISS).

['"Draft Genome Sequences of isolates belonging to family Methylobacteriaceae Isolated from the International Space Station"']

Rubinsicoccus jplei genome a novel genus isolated from JPL spacecraft environment where Mars 2020 subsystems are assembled.

The BRIC-21 mission was designed to identify the response of Bacillus subtilis to the human spaceflight environment. For this mission samples were grown in rich-medium using the Biological Research in Canister Petri Dish Fixation Units (BRIC-PDFU) spaceflight hardware. B. subtilis spores were inoculated during spaceflight grown at the ambient ISS temperature and frozen in the onboard -80 C freezer prior to returning to Earth. RNA was extracted from samples grown onboard the International Space Station (ISS) and matching Ground Controls for transcriptome analysis.

['"Draft Genome Sequences of Sphingomonas sp. Isolated from the International Space Station"']

The microbial tracking-1 (MT-1) investigation allowed the characterization of the microbial population aboard the International Space Station (ISS).

Bacillus pumilus SAFR-032, an endospore-forming bacterial strain, was investigated to determine its methylation pattern (methylome) change, compared to ground control, after direct exposure to space conditions onboard the International Space Station (ISS) for 1.5 years. The resulting ISS-flown and non-flown strains were sequenced using the Nanopore MinION and an in-house method and pipeline to identify methylated positions in the genome. Our analysis indicated genomic variants and m6A methylation increased in the ISS-flown SAFR-032. To complement the broader omics investigation and explore phenotypic changes, ISS-flown and non-flown strains were compared in a series of laboratory-based chamber experiments using an X-ray irradiation source (doses applied at 250, 500, 750, 1000 and 1250 Gy); results show a potentially higher survival fraction of ISS-flown DS2 at the two highest exposures. Taken together, results from this study document lasting changes to the genome by methylation, potentially triggered by conditions in spaceflight, with functional consequences for the resistance of bacteria to stressors expected on long-duration missions beyond low Earth orbit.

['We report here the draft genome sequences of four strains isolated from spacecraft-associated surfaces exhibiting increased resistance to stressors such as UV radiation and exposure to H2O2. The draft genomes of strains 1P01SC, FO-92, 50v1, and 2P01AA had sizes of 5,500,894 bp, 4,699,376 bp, 3,174,402 bp, and 4,328,804 bp, respectively.']