Arabidopsis thaliana was evaluated for its response to the spaceflight environment in three replicated experiments on the International Space Station. Two approaches were used; GFP reporter genes were used to collect gene expression data in real time within unique GFP imaging hardware and plants were harvested on orbit to RNAlater for subsequent analyses of gene expression with using Affymetrix and SAGE transcriptome analyses. Three tissue types were examined (leaves hypocotyls and roots) and compared to analyses conducted with whole plants. Transcriptome analyses with whole plants suggested that the spaceflight environment had little impact on the transcriptome of arabidopsis however closer examination of selected tissues revealed that there are a number of tissue-specific responses that arabidopsis employs to respond to this novel environment

Arabidopsis thaliana was evaluated for its response to the spaceflight environment in three replicated experiments on the International Space Station. Two approaches were used; GFP reporter genes were used to collect gene expression data in real time within unique GFP imaging hardware and plants were harvested on orbit to RNAlater for subsequent analyses of gene expression with using Affymetrix and SAGE transcriptome analyses. Three tissue types were examined (leaves hypocotyls and roots) and compared to analyses conducted with whole plants. Transcriptome analyses with whole plants suggested that the spaceflight environment had little impact on the transcriptome of arabidopsis however closer examination of selected tissues revealed that there are a number of tissue-specific responses that arabidopsis employs to respond to this novel environment

These data include field observations from Northern Gulf of Mexico research surveys, as well as hydrodynamic and water quality model data generated by the Coastal Generalized Ecosystem Model (CGEM) between 2003-2007.

This dataset holds Level 1 (L1) brightness temperature data collected by the Hyperspectral Thermal Emission Spectrometer (HyTES) instrument. This imagery was acquired as part of the Surface Biology and Geology High-Frequency Time Series (SHIFT) campaign on March 23, 2022. The SHIFT campaign generated precise, high-frequency data on plant communities for nearly 1,656 square kilometers across Santa Barbara County, California, US, and the nearby ocean. HyTES is a compact image spectrometer that acquires data in 256 spectral bands between 7.5 and 12 micrometers; it was deployed on a Twin Otter aircraft. The SHIFT campaign sought to demonstrate the joint use of both VSWIR and thermal infrared (TIR) data. TIR data are used to measure land surface temperature (LST), which informs models of water flux from land surface through processes such as evapotranspiration. LST is sensitive to solar heat gains and local cooling effects due to evaporative cooling. The HyTES instrument measures TIR radiances that can be used to derive LST, emissivity and Level 3 products such as latent heat flux and detection of air pollution sources. The HyTES data are provided in HDF5 format and include 91 flight scenes. The data are not projected, but georeferencing information for each pixel are provided in the HDF5 and a separate ENVI file for each flight scene. In addition, the flight scene boundaries and an overlay image are provided in Keyhole Markup Language (KML) along with a quicklook image and spectral response data.

Background Vacuolar type H+-ATPases play a critical role in the maintenance of vacuolar homeostasis in plant cells. V-ATPases are also involved in plants' defense against environmental stress. This research examined the expression and regulation of the catalytic subunit of the vacuolar type H+-ATPase in Arabidopsis thaliana and the effect of environmental stress on multiple transcripts generated by this gene. Results Evidence suggests that subunit A of the vacuolar type H+-ATPase is encoded by a single gene in Arabidopsis thaliana. Genome blot analysis showed no indication of a second subunit A gene being present. The single gene identified was shown by whole RNA blot analysis to be transcribed in all organs of the plant. Subunit A was shown by sequencing the 3' end of multiple cDNA clones to exhibit multi site polyadenylation. Four different poly (A) tail attachment sites were revealed. Experiments were performed to determine the response of transcript levels for subunit A to environmental stress. A PCR based strategy was devised to amplify the four different transcripts from the subunit A gene. Conclusions Amplification of cDNA generated from seedlings exposed to cold, salt stress, and etiolation showed that transcript levels for subunit A of the vacuolar type H+-ATPase in Arabidopsis were responsive to stress conditions. Cold and salt stress resulted in a 2–4 fold increase in all four subunit A transcripts evaluated. Etiolation resulted in a slight increase in transcript levels. All four transcripts appeared to behave identically with respect to stress conditions tested with no significant differential regulation.

The hyporheic zone (i.e., where surface & groundwater converge) is a geomorphic feature that contributes to the overall health of streams & rivers. Much of the function of the hyporheic zone depends on microbes, that modify & recycle nutrients & other biological molecules. How the hyporheic zone is altered in stream restorations is understudied. This project attempts to document changes microbial activity & community structure in restored reaches of an urban stream, Thornton Creek in Seattle, including attempts to "seed" or accelerate population of newly installed hyporheic elements by transplanting hyporheic material from a pristine system, the Cedar River watershed. Assessment of the microbial component is being performed in concert with assessments of macroinvertebrate communities & water quality measures. Filemaker Pro database of results from field & lab effort.

These plant and soil data were collected by Timothy M. Wertin and Sasha C. Reed in the spring, summer, and fall of 2011 at a climate manipulation experiment site near Moab, UT (38.521411, -109.470567). These data were collected to assess how warming affects leaf photosynthesis, soil CO 2 efflux, and soil chemistry in plots of ambient and warming treatments.

Contains the HYD-08 weights of the dried moss samples. These weights do not include the weight of the tray in which the sample was contained. Contains the HYD-08 moss measurements of water equivalent made at the moss lysimeter sites near the SSA-OBS in 19

,A native solitary bee to North America, the blue orchard bee (Osmia lignaria Say, Hymenoptera: Megachilidae) is a crucial pollinator for orchard crops such as apples, almonds, and cherries. Osmia lignaria is often managed commercially and sold to complement honey bee pollination services.,We collected data following an accidental drop of developing immature bees inside their cocoons. These bees were part of a larger experiment performed in 2020. On June 17, 2020, bees were dropped approximately one meter onto a linoleum floor at the USDA-ARS-PWA Pollinating Insect Research Unit in Logan, Utah, USA. Developing bees were in gelatin capsules and attached to a sticky board for X-ray imaging. Using a board from the same study that had not fallen, we compared survival, life stages, and bodily injuries to document the effects of dropping immature O. lignaria a short distance.,Our research highlights the risks of handling immature O. lignaria during metamorphosis. Our data provides valuable information for bee managers and researchers about the risks of physical disturbances during critical developmental stages, which could affect bee survival and pollination services in orchards.,Key findings include: (1) Near-complete mortality of developing bees before the adult molt stage, (2) Insights into the vulnerability of O. lignaria during immature developmental stages, even when inside cocoons, and (3) Documentation of how mechanical injury during immature development impacts survival.,The dataset provides counts of bees in different life stages and conditions, including: (1) Life status (alive or dead) at cocoon completion, pupation, and adult molt stages, (2) Sex determination for bees that reached adulthood (male or female), (3) Final life stage reached (prepupa, pupa, or adult), and (4) Body condition after the fall (malformed, melanized, no observable change, or partially melanized).,Additional variables in the dataset include: (1) Sample identifiers, treatment groups, and X-ray board identifiers from the original experiment and (2) Whether the board was dropped or not.,Abbreviations and acronyms in the dataset,,