The data set provides all data that were reported in results, tables, and figures associated with Stinckens et al., "The effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish". The data set includes: 1) Effects of three test chemicals on thyroperoxidase enzyme activity 2) Effects of three test chemicals on deiodinase enzyme activity 3) Thyroid hormone (T3 and T4) concentrations measured in exposed larvae 4) Measures of posterior and anterior swimbladder chamber inflation and surface areas. 5) Data on swimming behavior (distance) of exposed fish. 6) Measured concentrations of the test chemicals in the test media and tissues. This dataset is associated with the following publication: Stinckens, E., L. Vergauwen, B. Blackwell, G. Ankley, D. Villeneuve, and D. Knapen. Effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(10): 6213-6223, (2020).

The data set includes: 1) Swimbladder inflation at 120 hours post-fertilization (hpf) 2) Swimbladder surface area at 120 hpf 3) Larval body length at 120 hpf 4) Swimming activity at 120 hpf 5) Targeted whole body gene expression results for nine mRNA transcripts evaluated at 60 hpf (hb9; fgf10a; acta2; anxa5b; wnt5b; fzd2; shha; ihha; ptc1) 6) Targeted whole body gene expression results for the same nine mRNA transcripts evaluated at 120 hpf. 7) Whole body thyroid hormone concentrations measured at 48, 60, 96, and 120 hpf 8) Whole body thyroid hormone concentrations in IOP exposed embryos/larvae at 48, 60, and 120 hpf 9) Data for supplementary figures S6, S7A, S7B 10) Ratio T3 of IOP exposed embryos over control T3 levels during embryonic-juvenile development (4 replicates, Figure S9). This dataset is associated with the following publication: Van Dingenen, I., L. Vergauwen, A. Haigis, B. Blackwell, E. Stacy, D. Villeneuve, and D. Knapen. Deiodinase inhibition impairs the formation of the three posterior swim bladder tissue layers during early embryonic development in zebrafish. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 261: 106632, (2023).

The present study investigated whether inhibition of deiodinase, the enzyme which converts thyroxine (T4) to the more biologically-active form, 3,5,3'-triiodothyronine (T3), would impact inflation of the posterior and/or anterior chamber of the swim bladder, processes previously demonstrated to be thyroid-hormone regulated. Two experiments were conducted using a model deiodinase inhibitor, iopanoic acid (IOP). In the first study, fathead minnow (Pimephales promelas) embryos were exposed to 0.6, 1.9, or 6.0 mg IOP/L or control water in a flow-through system until reaching 6 days post-fertilization (dpf) at which time posterior swim bladder inflation was assessed. To examine effects on anterior swim bladder inflation, a second study was conducted with 6 dpf larvae exposed to the same IOP concentrations until reaching 21 dpf. Fish from both studies were sampled for T4/T3 measurements, gene transcription analyses, and thyroid histopathology. In the embryo study, incidence and length of inflated posterior swim bladders were significantly reduced in the 6.0 mg/L treatment at 6 dpf. Incidence of inflation and length of anterior swim bladder in larval fish were significantly reduced in all IOP treatments at 14 dpf, but inflation recovered by 18 dpf. Throughout the larval study, whole body T4 concentrations were significantly increased and T3 concentrations were significantly decreased in all IOP treatments. Consistent with hypothesized compensatory responses, significant up-regulation of deiodinase-2 mRNA was observed in the larval study, and down-regulation of thyroperoxidase mRNA was observed in all IOP treatments in both studies. Taken together, these results support the hypothesized adverse outcome pathways linking inhibition of deiodinase activity to impaired swim bladder inflation. This dataset is associated with the following publication: Cavallin, J., G. Ankley, B. Blackwell, C. Blanksma, K. Fay, K. Jensen, M. Kahl, D. Knapen, P. Kosian, S. Poole, E. Randolph, A. Schroeder, L. Vergauwen, and D. Villeneuve. Impaired swim bladder inflation in early-life stage fathead minnows exposed to a deiodinase inhibitor, iopanoic acid (article). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 36(11): 2942-2952, (2017).

The present study investigated whether inhibition of deiodinase, the enzyme which converts thyroxine (T4) to the more biologically-active form, 3,5,3'-triiodothyronine (T3), would impact inflation of the posterior and/or anterior chamber of the swim bladder, processes previously demonstrated to be thyroid-hormone regulated. Two experiments were conducted using a model deiodinase inhibitor, iopanoic acid (IOP). In the first study, fathead minnow (Pimephales promelas) embryos were exposed to 0.6, 1.9, or 6.0 mg IOP/L or control water in a flow-through system until reaching 6 days post-fertilization (dpf) at which time posterior swim bladder inflation was assessed. To examine effects on anterior swim bladder inflation, a second study was conducted with 6 dpf larvae exposed to the same IOP concentrations until reaching 21 dpf. Fish from both studies were sampled for T4/T3 measurements, gene transcription analyses, and thyroid histopathology. In the embryo study, incidence and length of inflated posterior swim bladders were significantly reduced in the 6.0 mg/L treatment at 6 dpf. Incidence of inflation and length of anterior swim bladder in larval fish were significantly reduced in all IOP treatments at 14 dpf, but inflation recovered by 18 dpf. Throughout the larval study, whole body T4 concentrations were significantly increased and T3 concentrations were significantly decreased in all IOP treatments. Consistent with hypothesized compensatory responses, significant up-regulation of deiodinase-2 mRNA was observed in the larval study, and down-regulation of thyroperoxidase mRNA was observed in all IOP treatments in both studies. Taken together, these results support the hypothesized adverse outcome pathways linking inhibition of deiodinase activity to impaired swim bladder inflation. This dataset is associated with the following publication: Cavallin, J., G. Ankley, B. Blackwell, C. Blanksma, K. Fay, K. Jensen, M. Kahl, D. Knapen, P. Kosian, S. Poole, E. Randolph, A. Schroeder, L. Vergauwen, and D. Villeneuve. Impaired swim bladder inflation in early-life stage fathead minnows exposed to a deiodinase inhibitor, iopanoic acid (article). ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 36(11): 2942-2952, (2017).

Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2006.

Data describe results of a set of laboratory experiments using a previously described quantitative thiaminase I activity assay to test the thiaminase activity of whole zebrafish homogenates. Parameters described include the thiaminase activity data, dilution factors, incubation time, and tissue weight assayed. Experiments were conducted at the USGS Columbia Environmental Research Center, Columbia, MO in 2006.

In this study, a hypothesized adverse outcome pathway (AOP) linking inhibition of thyroid peroxidase (TPO) activity to impaired swim bladder inflation was investigated in experiments in which fathead minnows were exposed to the TPO inhibitor 2-mercaptobenzothiazole (MBT). Results show that anterior, but not posterior, swim bladder inflation was impacted by exposure to MBT supporting the development of an AOP linking a specific thyroid-disrupting molecular initiating event to a significant phenotypic outcome. Results also suggest an alternative short-term in vivo test with larval fathead minnows that could be used to screen chemicals for thyroid disrupting activity and possibly distinguish thyroid disrupting modes of action. The dataset contains information on TPO expression, thyroid hormone concentrations, and swim bladder inflation measurements in larval fathead minnows. This dataset is associated with the following publication: Nelson, K., A. Schroeder , G. Ankley , B. Blackwell, C. Blanksma, S. Degitz , K. Jensen , R. Johnson , M. Kahl , D. Knapen, P. Kosian , R. Milsk, E. Randolph, T. Saari, E. Stinckens, L. Vergauwen, and D. Villeneuve. Impaired anterior swim bladder inflation following exposure to the thyroid peroxidase inhibitor 2-Mercaptobenzothiazole Part I: Fathead minnow. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 173: 192-203, (2016).

In this study, a hypothesized adverse outcome pathway (AOP) linking inhibition of thyroid peroxidase (TPO) activity to impaired swim bladder inflation was investigated in experiments in which fathead minnows were exposed to the TPO inhibitor 2-mercaptobenzothiazole (MBT). Results show that anterior, but not posterior, swim bladder inflation was impacted by exposure to MBT supporting the development of an AOP linking a specific thyroid-disrupting molecular initiating event to a significant phenotypic outcome. Results also suggest an alternative short-term in vivo test with larval fathead minnows that could be used to screen chemicals for thyroid disrupting activity and possibly distinguish thyroid disrupting modes of action. The dataset contains information on TPO expression, thyroid hormone concentrations, and swim bladder inflation measurements in larval fathead minnows. This dataset is associated with the following publication: Nelson, K., A. Schroeder , G. Ankley , B. Blackwell, C. Blanksma, S. Degitz , K. Jensen , R. Johnson , M. Kahl , D. Knapen, P. Kosian , R. Milsk, E. Randolph, T. Saari, E. Stinckens, L. Vergauwen, and D. Villeneuve. Impaired anterior swim bladder inflation following exposure to the thyroid peroxidase inhibitor 2-Mercaptobenzothiazole Part I: Fathead minnow. AQUATIC TOXICOLOGY. Elsevier Science Ltd, New York, NY, USA, 173: 192-203, (2016).

Data used in the creation of each figure and table cited in Doering et al. (2019; DOI: 10.1021/acs.est.9b02606) are provided. This dataset is associated with the following publication: Doering, J., D. Villeneuve, S. Poole, B. Blackwell, K. Jensen, M. Kahl, A. Kittelson, D. Feifarek, C. Tilton, C. Lalone, and G. Ankley. Quantitative response-response relationships linking aromatase inhibition to decreased fecunditiy are conserved across three fishes with asynchronous oocyte development. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 53(17): 10470-10478, (2019).

Data used in the creation of each figure and table cited in Doering et al. (2019; DOI: 10.1021/acs.est.9b02606) are provided. This dataset is associated with the following publication: Doering, J., D. Villeneuve, S. Poole, B. Blackwell, K. Jensen, M. Kahl, A. Kittelson, D. Feifarek, C. Tilton, C. Lalone, and G. Ankley. Quantitative response-response relationships linking aromatase inhibition to decreased fecunditiy are conserved across three fishes with asynchronous oocyte development. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 53(17): 10470-10478, (2019).