Although endocrine disrupting compounds have been detected in wastewater and surface waters worldwide using a variety of in vitro effects-based screening tools, e.g. bioassays, few have examined potential attenuation of environmental contaminants by both natural (sorption, degradation, etc.) and anthropogenic (water treatment practices) processes. This study used several bioassays and quantitative chemical analyses to assess residence-time weighted samples at six sites along a river in the northeastern United States beginning upstream from a wastewater treatment plant outfall and proceeding downstream along the stream reach to a drinking water treatment plant. Known steroidal estrogens were quantified and changes in signaling pathway molecular initiating events (activation of estrogen, androgen, glucocorticoid, peroxisome proliferator-activated, pregnane X receptor, and aryl hydrocarbon receptor signaling networks) were identified in water extracts. In initial multi-endpoint assays geographic and receptor-specific endocrine activity patterns in transcription factor signatures and nuclear receptor activation were discovered. In subsequent single endpoint receptor-specific bioassays, estrogen (16 of 18 samples; 0.01 to 28 ng estradiol equivalents [E2Eqs]/L) glucocorticoid (3 of 18 samples; 1.8 to 21 ng dexamethasone equivalents [DexEqs]/L), and androgen (2 of 18 samples; 0.95 to 2.1 ng dihydrotestosterone equivalents [DHTEqs]/L) receptor transcriptional activation occurred above respective assay method detection limits (0.04 ng E2Eqs/L, 1.2 ng DexEqs/L, and 0.77 ng DHTEqs/L) in multiple sampling events. Estrogen activity, the most often detected, correlated well with measured concentrations of known steroidal estrogens (r2 = 0.890). Overall, activity indicative of multiple types of endocrine active compounds was highest in wastewater effluent samples, while activity downstream was progressively lower, and negligible in unfinished treated drinking water. Not only was estrogenic and glucocorticoid activity confirmed in the effluent by utilizing multiple methods concurrently, but other activated signaling networks that historically received less attention (i.e. peroxisome proliferator-activated receptor) were also detected. This dataset is associated with the following publication: Medlock Kakaley, E., B. Blackwell, M. Cardon, J. Conley, N. Evans, D. Feifarek, E. Furlong, S. Glassmeyer, L. Gray, P. Hartig, D. Kolpin, M. Mills, L. Rosenblum, D. Villeneuve, and V. Wilson. De Facto Water Reuse: Bioassay suite approach delivers depth and breadth in endocrine active compound detection. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 699(134297): 1, (2020).

This dataset compared the de facto reuse percentage modeled for the 22 surface water sites sampled in Phase II of the drinking water project and the organic chemical data generated as part of the project. This dataset is associated with the following publication: Nguyen , T., P. Westerhoff , E. Furlong, D. Kolpin, A. Batt, H. Mash, K. Schenck, J.S. Boone, J. Rice, and S. Glassmeyer. Modeled De Facto Reuse and Contaminants of Emerging Concern in Drinking Water Source Waters. JOURNAL OF THE AMERICAN WATER WORKS ASSOCIATION. American Water Works Association, Denver, CO, USA, 110(4): E2-E18, (2018).

This dataset compared the de facto reuse percentage modeled for the 22 surface water sites sampled in Phase II of the drinking water project and the organic chemical data generated as part of the project. This dataset is associated with the following publication: Nguyen , T., P. Westerhoff , E. Furlong, D. Kolpin, A. Batt, H. Mash, K. Schenck, J.S. Boone, J. Rice, and S. Glassmeyer. Modeled De Facto Reuse and Contaminants of Emerging Concern in Drinking Water Source Waters. JOURNAL OF THE AMERICAN WATER WORKS ASSOCIATION. American Water Works Association, Denver, CO, USA, 110(4): E2-E18, (2018).

The associated manuscript reports the results of a repeated sampling of a watershed, including surface water and drinking water, that were analyzed with multiple non-targeted analysis (NTA) methods. About 130 chemicals were tentatively identified in the environmental samples, with no concentrations being reported with the non-targeted analysis. The results of this manuscript provide users of NTA with important information on which types of chromatography and ionization methods can be used to detect different classes of environmental contaminants, including PFAS, pesticides, surfactants, and pharmaceuticals. The metadata published here includes a list of all unidentified chemical features detected by each non-target method tested. This dataset is associated with the following publication: Batt, A., L. Brunelle, N. Quinete, E. Stebel, B. Ng, P. Gardinali, A. Chao, A. Huba, S. Glassmeyer, D. Alvarez, D. Kolpin, E. Furlong, and M. Mills. Investigating the chemical space coverage of multiple chromatographic and ionization methods using non-targeted analysis on surface and drinking water collected using passive sampling. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 955: 176922, (2024).

Dataset summarizes the results of a study to examine de facto reuse of contaminants of emerging concern

Collection of proposed, operating, demonstration, and pilot study potable reuse systems across the U.S. as of 2020. Information includes project name, location, augmentation type, capacity, year operational, treatment train, and source water type. References are included in this submission. As potable reuse facilities undergo expansions or upgrades, treatment trains and capacities are subject to change. Operational status may also change. Treatment trains listed are reported to the detail that the source provides. Type and doses of chemical post-treatment or chlorination is often not specified. Please see README section in spreadsheet for definitions of acronyms used.

Collection of proposed, operating, demonstration, and pilot study potable reuse systems across the U.S. as of 2020. Information includes project name, location, augmentation type, capacity, year operational, treatment train, and source water type. References are included in this submission. As potable reuse facilities undergo expansions or upgrades, treatment trains and capacities are subject to change. Operational status may also change. Treatment trains listed are reported to the detail that the source provides. Type and doses of chemical post-treatment or chlorination is often not specified. Please see README section in spreadsheet for definitions of acronyms used.

A full list of non-target chemical features found in the study "De facto water reuse - Investigating the fate and transport of chemicals of emerging concern from wastewater discharge through drinking water treatment using non-targeted analysis and suspect screening." This would include all identified and unidentified chemicals found in a watershed with wastewater, surface water, and drinking water, chemicals that were found to survive drinking water treatment. This dataset is associated with the following publication: Brunelle, L., A. Batt, A. Chao, S. Glassmeyer, N. Quinete, D. Alvarez, D. Kolpin, E. Furlong, M. Mills, and D. Aga. De facto water reuse - Investigating the fate and transport of chemicals of emerging concern from wastewater discharge through drinking water treatment using non-targeted analysis and suspect screening. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 58(5): 2468-2478, (2024).

In vitro bioactivity concentrations and chemical concentrations of estrogens, androgens, and glucocorticoids from a pilot study of US tapwaters. In vitro bioassays include T47D-Kbluc, MDA-kb2, and a CV-1 cell line transduced with human glucocorticoid receptor. This dataset is associated with the following publication: Bradley, P., D. Kolpin, K. Romanok, K. Smalling, M. Focaszio, J. Brown, M. Cardon, K. Carpenter, S. Corsi, L. DeCicco, J. Dietze, N. Evans, E. Furlong, C. Givens, J. Gray, D. Griffin, C. Higgins, M. Hladik, L. Iwanowicz, C. Journey, K. Kuivila, J. Masoner, C. McDonough, M. Meyer, J. Orlando, M. Strynar, C. Weis, and V. Wilson. Reconnaissance of Mixed Organic and Inorganic Chemicals in Private and Public Supply Tapwaters at Selected Residential and Workplace Sites in the United States. Environmental Science & Technology Letters. American Chemical Society, Washington, DC, USA, 13972-13985, (2018).