All data associated with publication are publicly available online at https://datadryad.org/ by searching doi:10.5061/dryad.2tg6967 Upon accessing data on datadryad.org: Data descriptions are found with Supplemental Data presented in the pdf (Supplementary Data.pdf (600.4 Kb)). Data description of supplemental Data (Supplementary Data File.xlsx (1.167 Mb)) is provided below: Tabs one, two, and three contain the raw data for acetylcholinesterase (AChE) Level 1 (Figure 1A), Level 2 (Figure 1B), and Level 3 (Table 2) SeqAPASS analysis, respectively. Tab four contains the raw data for AChE Level 3 improved SeqAPASS analysis (Table 5). Tabs five, six, and seven contain the raw data for ecdysone receptor (EcR) Level 1 (Figure 2A), Level 2 (Figure 2B), and Level 3 (Table 6) SeqAPASS analysis, respectively. Tab eight contains the raw data for EcR Level 3 improved SeqAPASS analysis (Table 8). This dataset is associated with the following publication: Doering, J., S. Lee, K. Kristiansen, L. Evenseth, M. Barron, I. Sylte, and C. LaLone. In silico site-directed mutagenesis informs species-specific predictions of chemical susceptibility derived from the Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) tool.. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 166(1): 131-145, (2018).