A-3txf_sequence summary.xksx: Abundance of contigs or unique sequences for each biofilm samples from anodes in the MEC reactor Hodon Waterloo final_fasta_working.docx: Raw sequences with their identification numbers RNA S1_MEC.docx: Representative sequences with their ID number and taxonomy. This dataset is associated with the following publication: Santodomingo, J., H. Ryu, B. Dhar, and H. Lee. Ohmic resistance affects microbial community and electrochemical kinetics in a multi-anode microbial electrochemical cell. JOURNAL OF POWER SOURCES. Elsevier Science Ltd, New York, NY, USA, 331: 315-321, (2016).

Data from small lab scale tests conducted at ECBC. It contains efficacy data as well as data on env conditions such as temperature, RH, and hydrogen peroxide vapor concentration. This dataset is associated with the following publication: Wood, J., W. Calfee, S. Ryan, L. Mickelsen, M. Clayton, and V. Rastogi. A Simple Decontamination Approach Using Hydrogen Peroxide Vapor for Bacillus anthracis Spore Inactivation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(6): 1603-1615, (2016).

Data summary for each figure. This dataset is associated with the following publication: Lehmann, D., K. Krishnakuman, M. Batres, A. Hakola-Parry, N. Cokcetin, E. Harry, and D. Carter. A Cost Effective Colourimetric Assay for Quantifying Hydrogen Peroxide in Honey. Access Microbiology. Microbiology Society, London, UK, 1(10): e000065, (2019).

The attached extensive computational chemistry dataset involves detailed electronic structure (density functional theory - DFT) and kinetic calculation (master equation formalism) outputs for the reactions of isoprene and first generation oxidants with the hydroxyl radical. There are a total of 9 tabs in the Excel spreadsheet. The first two tabs provide the potential energy surfaces (PESs) of the isoprene+OH and isopOOH+OH (1st generation oxidant) reactions. The PESs are zero-point energy corrected and obtained at the M062x/maug-cc-pVTZ level of DFT. The third tab provides the reaction barriers for first and second generation 1,5-hydrogen atom shifts for two different isoprene peroxy radical isomers with several different DFT methods. The fourth and fifth tabs provide microcanonical rate constants for the reactions of isoprene and isopOOH with OH respectively. The remaining tabs give the rate constants for the 1,5-H shifts for four different isoprene peroxy radicals. The rate constants are computed using the M062x density functional and an average of 4 different DFT methods given in tab 3 for comparison. The average values are reported as the final rate constants determined by computational methods. This dataset is associated with the following publication: Piletic, I., R. Howell, L. Bartolotti, T. Kleindienst, S. Kaushik, and E. Edney. Multigenerational Theoretical Study of Isoprene Peroxy Radical 1–5-Hydrogen Shift Reactions that Regenerate HOx Radicals and Produce Highly Oxidized Molecules. JOURNAL OF PHYSICAL CHEMISTRY A. American Chemical Society, Washington, DC, USA, 123(4): 906-919, (2019).

This study assessed the conductivity of a Geobacter-enriched biofilm anode along with biofilm activity in a microbial electrochemical cell (MxC) equipped with two gold anodes (25 mM acetate medium), as different proton gradients were built throughout the biofilm. There was no pH gradient across the biofilm anode at 100 mM phosphate buffer (current density 2.38 A/m2) and biofilm conductivity (Kbio) was as high as 0.87 mS/cm. In comparison, an inner biofilm became acidic at 2.5 mM phosphate buffer in which approximately 80 μm of the inner biofilm anode was metabolically inactive. At this low phosphate buffer, Kbio significantly decreased by 0.27 mS/cm, together with declined current density of 0.64 A/m2. This work demonstrates that biofilm conductivity depends on metabolic activity of Geobacter in the conductive biofilm anode. The decreased Kbio at acidic environment implies the presence of multiple conduction-EET pathways in the biofilm anode. This dataset is associated with the following publication: Dhar, B., J. Sim, H. Ryu, H. Ren, J. Santodomingo, J. Chae, and H. Lee. Microbial Activity Influences Electrical Conductivity of Biofilm Anode. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 127: 230-238, (2017).

A biofilm anode acclimated with acetate, acetate+methane, and methane growth media for over three years produced a steady current density of 1.6-2.3 mA/m^2 in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor. Geobacter was the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanum and Methanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. A fluorescence in situ hybridization (FISH) image clearly showed a biofilm of bacteria and archaea, supporting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs correlated linearly to the methane-gas concentration in the range of 10% to 99.97% (R^2 ≥ 0.99) when the measurement was sustained for at least 50 min. Thus, cumulative coulombs over 50 min. could be used to quantify the methane concentration in gas samples. This dataset is associated with the following publication: Gao, Y., H. Ryu, B. Rittmann, A. Hussain, and H. Lee. Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer. Bioresource Technology. Elsevier Online, New York, NY, USA, 241: 979-984, (2017).