Data for Joan M. Hedge, et al., Influence of Methylene Blue or Dimethyl Sulfoxide on Larval Zebrafish Development and Behavior. Zebrafish. http://doi.org/10.1089/zeb.2023.0017. This dataset is associated with the following publication: Hedge, J., D. Hunter, E. Sanders, K. Jarema, J. Olin, K. Britton, M. Lowery, B. Knapp, S. Padilla, and B. Hill. Influence of Methylene Blue or Dimethyl Sulfoxide on Larval Zebrafish Development and Behavior. Zebrafish. Mary Ann Liebert, Inc., New Rochelle, NY, USA, 20(4): 132-145, (2023).

The instructions for analysis are described in Kolli et al. (2019) along with a sample Illumina sequence file available with a ready-to-run VirtualMachine that can also be accessed at http://toxicology.uga.edu/resources/dna_methylation_analysis/. Supporting data for sequencing and qPCR raw data are available at https://doi.org/10.5281/zenodo.8313356, sharing qPCR raw data files for the endpoint for male and female fish from CFX Manger (Ver. 3.0), qPCR quantification data, TGBS raw sequences, and a TGBS metadata file with sample information. This dataset is associated with the following publication: Kolli, R., T. Glenn, R. Bringolf,, W. Henderson, B. Cummings, and J. Kenneke. Changes in CpG Methylation of the Vitellogenin 1 Promoter in Adult Male Zebrafish after Exposure to 17α-Ethynylestradiol. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 43(7): 1547-1556, (2024).

This file contains data used to generate figures shown in Catron et al. Characterization of host toxicity and microbiota disruption in larval zebrafish following developmental exposure to BPA and BPA alternatives. This dataset is associated with the following publication: Catron, T., S. Keely, N. Brinkman, T. Zurlinden, C. Wood, J. Wright, D. Phelps, E. Wheaton, A. Kvasnicka, S. Gaballah, R. Lamendella, and T. Tal. Host Developmental Toxicity of BPA and BPA Alternatives Is Inversely Related to Microbiota Disruption in Zebrafish. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 167(2): 468-483, (2019).

Figure S1: BMC curves. Table S1: Fish assessments. Table S2: Data to make visualization of toxicity (Figure 3). Table S3: Data to make BMC curves. This dataset is associated with the following publication: Britton, K., R. Judson, B. Hill, K. Jarema, J. Olin, B. Knapp, M. Lowery, M. Feshuk, J. Brown, and S. Padilla. Using Zebrafish to Screen Developmental Toxicity of Per- and Polyfluoroalkyl Substances (PFAS). Toxics. MDPI, Basel, SWITZERLAND, 12(7): 501, (2024).

Supporting Information for "Gust KA, Mylroie JE, Kimble AN, Wilbanks MS, Steward CSC, Chapman KA, Jensen KM, Kennedy AJ, Krupa PM, Waisner SA, Pandelides Z, Garcia-Reyero N, Erickson RJ, Ankley GT, Conder J, Moore DW. Survival, Growth, and Reproduction Responses in a Three-Generation Exposure of the Zebrafish (Danio rerio) to Perfluorooctane Sulfonate. Environ Toxicol Chem. 2024 Jan;43(1):115-131. doi: 10.1002/etc.5770. Epub 2023 Nov 29. PMID: 38018867.". This dataset is associated with the following publication: Gust, K., J. Mylroie, A. Kimble, M. Wilbanks, C. Steward, K. Chapman, K. Jensen, A. Kennedy, P. Krupa, S. Waisner, Z. Pandelides, N. Garcia-Reyero, R. Erickson, G. Ankley, J. Conder, and D. Moore. Survival, Growth, and Reproduction Responses in a Three-Generation Exposure of the Zebrafish (Danio rerio) to Perfluorooctane Sulfonate. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 43(1): 115-131, (2024).

The environmental fate, persistence, and point-source discharge of traditional molluscicidal compounds led to the development of an alternative biomolluscicide, Zequanox. Previous studies evaluated the efficacy and non-target animal safety of Zequanox in laboratory, mesocosm, and field enclosure studies. One study indicated sensitivity of salmonid species and lake sturgeon (Acipenser fulvescens Rafinesque 1817) following exposure to Zequanox, however, the exposures were not conducted in a manner consistent with the product label. This laboratory study evaluated sublethal and lethal impacts of Zequanox on lake sturgeon and lake trout (Salvelinus namaycush Walbaum in Artedi, 1792) following exposures that were conducted consistent with a Zequanox open-water label application. Fish were exposed to 50 and 100 mg Zequanox active ingredient/L for 8 h and then held for an additional 33-d for post-exposure observation. No acute mortality was observed in either species, however, significant latent mortality (46.2%) was observed in lake trout that were exposed to 100 mg of Zequanox active ingredient/L for 8 h. At the termination of the 33d holding period, biologically minimal, yet statistically significant, differences were observed in the terminal weight of surviving lake sturgeon (range 20.17 to 21.49 g) and biologically, as well as, statistically significant differences were observed in the terminal weight of surviving lake trout (range 6.19 to 9.55 g). Histological evaluation of lake trout gastrointestinal tracts suggest that a different mode of action is responsible for the Zequanox exposure-related impacts to lake trout than the mode of action that induces zebra and quagga mussel mortality. Further research is required to determine if Zequanox sensitivity is limited to lake trout or if all salmonid species are vulnerable to exposure and to determine if native fish will avoid Zequanox exposure.

The general objective of the study was to determine modulation of gene expression by environmental factors with a special emphasis on bone formation. For this reason the specific period of treatment was chosen between 5-6 days post-fertilization (dpf) when bone formation and calcification are taking place. This experiment was designed as a new type of gravitational experiment which we like to call relative microgravity referring to the fact that the larvae first grow in hyper gravity for 5 days and are then returned to 1g normal gravity for 1 day. Zebrafish embryos were placed on a Large Diameter Centrifuge at 3 hpf brought to a gravitational force of 3 g until 5 dpf. Reference embryos were kept in parallel at 1g (Inc). At 5dpf one batch was left at 3g (3g) one batch was returned to 1g (3g>1g) while a third batch was returned to 1g but left on the axis of the centrifuge (Axe; 3g>Axe). The experiment was repeated 4 times each time with 4 batches of 60 larvae.

The data set provides all data that were reported in results, tables, and figures associated with Stinckens et al., "The effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish". The data set includes: 1) Effects of three test chemicals on thyroperoxidase enzyme activity 2) Effects of three test chemicals on deiodinase enzyme activity 3) Thyroid hormone (T3 and T4) concentrations measured in exposed larvae 4) Measures of posterior and anterior swimbladder chamber inflation and surface areas. 5) Data on swimming behavior (distance) of exposed fish. 6) Measured concentrations of the test chemicals in the test media and tissues. This dataset is associated with the following publication: Stinckens, E., L. Vergauwen, B. Blackwell, G. Ankley, D. Villeneuve, and D. Knapen. Effect of thyroperoxidase and deiodinase inhibition on anterior swim bladder inflation in the zebrafish. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 54(10): 6213-6223, (2020).

This study continues our investigations into the effects of lampricides on mussels by extending research into potential effects on behavioral and reproductive endpoints on the plain pocketbook mussel(Lampsilis cardium). We hypothesized that TFM (3-trifluoromethyl-4'-nitrophenol) and TFM with Niclosamide (NIC, 2', 5-dichloro-4'-nitrosalicylanilide, hereafter TFM:NIC) would reduce the viability of free glochidia in a dose-dependent manner, that older glochidia would be more sensitive than younger glochidia, and that exposure of gravid females to TFM and TFM:NIC would cause a suite of behavioral and reproductive effects. These data could be important in documenting the presence (or absence) of potential behavioral and reproductive effects of lampricides on mussels and could inform management decisions. For example, these data could be used by the state of Michigan and the USFWS to evaluate the potential for sea lamprey treatments in the Grand River watershed (Grand Rapids, Michigan) – which contains several federally and state listed mussels – in response to a dam removal. The data can also be used to inform risk assessments for lampricides and listed species.