We evaluated the influence of diurnal carbonate chemistry variability on the bioerosion rates of two Caribbean sponges: the zooxanthellate Cliona varians and azooxanthellate Cliothosa delitrix. Sponge samples were exposed to four precisely-controlled pH treatments: contemporary static (8.05 ± 0.00; mean pH ± diurnal pH oscillation), contemporary variable (8.05 ± 0.10), future OA static (7.80 ± 0.00), and future OA variable (7.80 ± 0.10). Tank pH conditions measured throughout the entirety of the experiment are provided in the "fullExperiment_pHData.xlsx" file. Significantly enhanced bioerosion rates, determined using buoyant weight measurements, were observed under more variable conditions in both the contemporary and OA scenarios for C. varians, whereas the same effect was only apparent under contemporary pH conditions for C. delitrix. Buoyant weight data is provided in the "Buoyant_Weight_Data_Submission.xlsx" file.

This dataset includes lab-based measurements of ocean acidification on Caribbean bioeroders (endolithic algae and reef-excavating sponges) collected on Cheeca Rocks Reef, Florida Keys, Northwest Atlantic Ocean, from 2018-06-11 to 2018-07-12. Caribbean coral reef ecosystems have entered a state of net erosion in response to ocean acidification (OA) due to a combination of reduced carbonate production and enhanced bioerosion. The negative response of coral reef calcifiers to OA has been well-established, whereas OA-enhanced bioerosion is relatively poorly understood. Microboring algae and macroboring sponges are both major contributors to coral reef carbonate budgets (Perry et al., 2012). Microboring algae use exclusively chemical (extracellular ion transport) means (Garcia-Pichel, 2006) to break down carbonate framework, whereas macroboring sponges use a combination of both chemical (enzymatic dissolution) and mechanical (substrate dislodgment) methods (Rutzler and Rieger, 1973) to erode reef framework. Prior studies have found that both microboring algae and macroboring sponges appear to benefit from OA through both enhanced bioerosion and physiological fitness, but have disproportionally focused on the responses of Pacific Ocean species. Here, we independently evaluated the OA-response of two Caribbean bioeroders to quantify the impact of OA on their physiology and bioerosion rates.

During the summers of 2022 and 2023, U.S. Geological Survey (USGS) researchers conducted underwater surveys aimed at quantifying the impact that past coral restoration by Mote Marine Laboratory had on the reef-accretion process. The surveys were conducted along paired transects representing restored and non-restored areas of eight offshore reefs and three patch-reef sites in the Lower Florida Keys. At each location on each reef, USGS researchers conducted photographic surveys (these images are published in an accompanying release by Johnson and others, 2025) to generate structure-from-motion (SfM) products (point clouds, orthomosaics, and digital surface models [DSMs]), which were used to quantify percent cover of corals and other benthos and topographic complexity. USGS researchers also conducted census surveys of bioeroding parrotfishes, urchins, and sponges at each site.

The files in this this U.S. Geological Survey (USGS) data release (Kellogg and others, 2021) are the raw 16S ribosomal ribonucleic acid (rRNA) gene amplicon deoxyribonucleic acid (DNA) sequence files from 90 samples of tropical and cold-water corals, as well as sequence files from a mock community and extraction blanks for the kits used for DNA extraction. The mock community was sequenced in order to assess any biases in the sequencing technology, while extraction blanks were sequenced in order to identify any contaminants in the DNA extraction kits. The tropical coral samples (three species) were collected by permit (#FKNMS-2017-064) in March 2018 from a nursery in the Florida Keys National Marine Sanctuary. The cold-water coral samples (two species) were collected in August 2018 from two locations in the Atlantic Ocean.

This dataset contains laboratory experimental data that were collected to examine the effects of elevated levels of carbon dioxide on the growth of Atlantic surfclam (Spisula solidissima), a species that supports both commercial and recreational fisheries in the Northeast United States. Three levels of carbon dioxide enrichment (low, medium, and high) were delivered to surfclams in a 12-week exposure experiment. All treatments were done in 3 replicates (A, B, C). Approximately every 2 to 3 weeks, 12 individuals were removed from each treatment and measurements of length, width, height, dry tissue, and dry shell were recorded. Length was measured across the longest part of the shell, parallel to the hinge. Width was the thickness of the shell, and height was measured form the hinge to the outer edge of the shell. Dry tissue and dry shell samples were dried at 60°C until constant weight was achieved (~5 days). DIC measurements of carbon dioxide enrichment were taken and analyzed on an Apollo SciTech, while pH was measured weekly with a spectrophotometer. Values reported for DIC, pH, temperature, and salinity are the mean of each treatment during the 12-week experiment. The data indicated that increased carbon dioxide affected growth, tissue mass, and shell weight for Atlantic surfclam.

This dataset contains laboratory experimental data that were collected to examine the effects of elevated levels of carbon dioxide on the growth of Atlantic surfclam (Spisula solidissima), a species that supports both commercial and recreational fisheries in the Northeast United States. Three levels of carbon dioxide enrichment (low, medium, and high) were delivered to surfclams in a 12-week exposure experiment. All treatments were done in 3 replicates (A, B, C). Approximately every 2 to 3 weeks, 12 individuals were removed from each treatment and measurements of length, width, height, dry tissue, and dry shell were recorded. Length was measured across the longest part of the shell, parallel to the hinge. Width was the thickness of the shell, and height was measured form the hinge to the outer edge of the shell. Dry tissue and dry shell samples were dried at 60°C until constant weight was achieved (~5 days). DIC measurements of carbon dioxide enrichment were taken and analyzed on an Apollo SciTech, while pH was measured weekly with a spectrophotometer. Values reported for DIC, pH, temperature, and salinity are the mean of each treatment during the 12-week experiment. The data indicated that increased carbon dioxide affected growth, tissue mass, and shell weight for Atlantic surfclam.

This dataset consists of dissolved inorganic carbon, total alkalinity, water temperature and salinity from laboratory analyzed discrete water samples taken at the Central Gulf of Mexico Ocean Observing System buoy moored at 28.9N, 90.5W off Coastal Louisiana from 2017-07-14 to 2022-02-20. These are validation samples for the NOAA Pacific Marine Environmental Laboratory (PMEL) Moored Autonomous pCO2 (MAPCO2®) system buoy CoastalLA_91W_30N, that includes SeaBird CTD and SAMI pH instruments.

This dataset contains laboratory experiment data that were collected to examine the effects of ocean acidification on the Atlantic surfclam, Spisula solidissima, a species worth $31 million in 2009. Ocean acidification has negatively impacted growth and survival of multiple bivalve species, but because each species and developmental stage can show different responses, these studies were designed to determine potential impacts of increased CO2 on the larvae of the commercially important surfclam. Additionally, the role of nutrition (i.e., phytoplankton concentration) was included in a portion of these experiments because food availability may be able to mitigate the stress of ocean acidification and because ocean acidification has the potential to impact marine phytoplankton communities. During the summer of 2011, three different experiments were conducted at Woods Hole Oceanographic Institution examining the effects of three different pCO2 concentrations on larval surf clams. Two short term experiments (~70h) examined the effect of food availability on early shell development (fed vs unfed). One long term experiment (~21d) was conducted to examine the effects of pCO2 on shell development and metamorphic success (all animals well fed). Carbonate data is reported from these preliminary short-term experiments, and survival and shell length data is reported, in addition to carbonate data, from the long-term experiment. During 2012, one 6 day experiment was conducted examining the role and potential interactive effects of high and low food availability (400 and 40,000 cells ml-1 Tiso) and differential CO2 concentrations (ambient, ~1200 ppm and ~2200ppm). From these experiments, carbonate data, shell length, mass and biochemical compositions are reported. In 2013, two additional experiments were conducted to confirm results obtained in 2012. Unfortunately we observed stunted larval growth, no feeding effect on growth, high mortalities and a general failure to thrive. Given this, we infer poor gamete quality may have been the cause, and have chosen not to interpret these data as results are suspect. Therefore, 2013 data are therefore not included in this data submission.

Coral cores collected nearby the Atlantic Ocean Acidification Test-bed (AOAT) at La Parguera, Puerto Rico were used to characterize the relationship between paleo-variations in coral growth and calcification and seawater pH via the boron isotope proxy. This study addressed impacts of ocean acidification in a geological context to quantify baseline variability in growth and pH and assess the historical response of coral ecosystems to increased atmospheric CO2 and enhance on-going AOAT observations.

The U.S. Geological Survey St. Petersburg Coastal and Marine Science Center’s (USGS SPCMSC) Core Archive in St. Petersburg, FL contains a collection of coral-reef cores collected from throughout the Florida Keys reef tract (FKRT). In a previous study (Toth and Stathakopoulos, 2019), USGS researchers analyzed the upper, Holocene (~11,700 years ago to present) sections of those cores to evaluate how the coral composition of the FKRT changed over millennial timescales. Using the same methods, USGS researchers quantified the relative composition of late Pleistocene (~116 to 74 thousand years before present; Marine Isotope Stages [MIS] 5d, 5c, 5b, and 5a) sections of the coral reef cores dated by Hsia and others (2024a,b). This data release provides metadata about the location of the cores and summarizes the relative composition of coral taxa and other carbonates and the water depths (relative to modern mean sea level) of the analyzed core intervals. The data release also provides a summary of previously unpublished data (collected by David Weinstein) on the relative composition of an older Late Pleistocene reef (growing ~130–116 thousand years before present; MIS5e) from the subaerially exposed fossil reef at Windley Key Fossil Reef Geological Park. These data are compared with Holocene and modern coral-reef assemblages on the FKRT in Toth and others (2025).