In 2012, a program was initiated using in-stream and aerial (whole-watershed) liming to improve water quality and Brook Trout (Salvelinus fontinalis) recruitment in three acidified tributaries of a high-elevation Adirondack lake in New York State. Concurrently, macroinvertebrates were sampled annually between 2013 and 2016 at 3 treated and 3 untreated reference sites to assess the effects of each liming technique on this community. Macroinvertebrate communities were monitored at 6 study sites: T16, T8A (50 m upstream of lime application point), T8 (50 m downstream of lime application point), T6 (1230 m downstream of the lime application point), and at two unlimed reference streams, T24 and T20. T24 is of similar orientation, drainage area, discharge, and water chemistry as T16 and was selected as a reference site to assess the impacts of the watershed liming. T20 is a relatively well-buffered tributary that was monitored as a reference site for the in-stream liming effort. This dataset includes macroinvertebrate community data from 4-years (2013-2016) of macroinvertebrate sampling using artificial substrate basket samplers at six sites on tributaries to Honnedaga Lake, NY. Baskets were deployed in pairs at five stations (replicates) distributed longitudinally within each site (10 total baskets per site) and were placed on the bottom in pools where they were unlikely to become desiccated during water level fluctuations. Baskets were deployed between May 12 and May 16 and retrieved between July 10 and July 17 during each year, resulting in a colonization period of approximately two months. At the end of the colonization period, macroinvertebrates were extracted from each basket through a shaking and rinsing process. The contents from each pair of baskets were preserved together in 95-percent ethanol, resulting in 5 replicate samples collected from each site. A 200-organism subsample, or an exhaustive pick when less than 200 organisms were present, was sorted from each replicate using a gridded tray and identified to the lowest possible taxonomic resolution (usually genus or species). These identifications were then used to generate metrics of macroinvertebrate community condition for subsequent analyses. Data are provided in CSV and XLSX (MS Office 2013) format, a sample site location map is also provided (latitude/longitude datum and projection: NAD 1983 UTM Zone 18N).

Stream water chemistry and benthic macroinvertebrate data collected in the North Fork of Clear Creek and Clear Creek, CO, USA before and after the diversion and treatment of acid mine drainage by a high-density sludge lime process water treatment plant. Stream water chemistry and benthic macroinvertebrate samples were collected from November 2011 through October 2019 at 6 sites located above and below the North Clear Creek Water Treatment Plant (NCCWTP). The NCCWTP became fully operational in September 2017. Measured water chemistry included pH, conductivity, alkalinity, ferrous iron, total and dissolved concentrations of metals and major geochemical cations, and dissolved organic carbon. Replicate (n=5) benthic macroinvertebrate samples were collected using a 0.1 square meter Hess sampler at targeted riffle habitats among sites. Benthic samples were processed from detrital material in the laboratory using a subsampling technique to reach 300+ invertebrates, and identified to the lowest taxonomic resolution possible, typically to genus (except for chironomids that were identified to subfamily).

Stream water chemistry and benthic macroinvertebrate data collected in the North Fork of Clear Creek and Clear Creek, CO, USA before and after the diversion and treatment of acid mine drainage by a high-density sludge lime process water treatment plant. Stream water chemistry and benthic macroinvertebrate samples were collected from November 2011 through October 2019 at 6 sites located above and below the North Clear Creek Water Treatment Plant (NCCWTP). The NCCWTP became fully operational in September 2017. Measured water chemistry included pH, conductivity, alkalinity, ferrous iron, total and dissolved concentrations of metals and major geochemical cations, and dissolved organic carbon. Replicate (n=5) benthic macroinvertebrate samples were collected using a 0.1 square meter Hess sampler at targeted riffle habitats among sites. Benthic samples were processed from detrital material in the laboratory using a subsampling technique to reach 300+ invertebrates, and identified to the lowest taxonomic resolution possible, typically to genus (except for chironomids that were identified to subfamily).

These data were used to describe temporal trends in fish and invertebrate communities in the Scientific Investigators Report: Evaluation and Review of Ecology-Focused Stream Studies to Support Cooperative Monitoring, Fountain Creek Basin, Colorado. This data release consists of invertebrate data collected between 1985 and 2022, fish data collected between 2003 and 2022, and the input and output files from the trend analyses.

These data were used to describe temporal trends in fish and invertebrate communities in the Scientific Investigators Report: Evaluation and Review of Ecology-Focused Stream Studies to Support Cooperative Monitoring, Fountain Creek Basin, Colorado. This data release consists of invertebrate data collected between 1985 and 2022, fish data collected between 2003 and 2022, and the input and output files from the trend analyses.

These data were used for an analysis as part of a long-term project monitoring the biological communities, habitat, and water quality of the Fountain Creek Basin. Biology, habitat, and water quality data have been collected at 10 sites starting in 2003. These data include annual samples of aquatic invertebrate communities, fish communities, water quality, and quantitative riverine habitat collected from 2003-2016. These data will provide insight into trends in biological communities from 2003-2016 and relationships between biological communities and abiotic variables (antecedent streamflow, physical habitat, and water quality). This USGS data release contains all the input and output data and files necessary to reproduce results of the report describing an analysis of ecological trends from 2003-2016 in the Fountain Creek Basin, Colorado, USA (https://doi.org/10.3133/sir20175162). Data preparation of these data files is fully described in the report mentioned previously. These data are outputs from the two-step trend analysis and provide the statistical measures of trend significance, potential drivers of trends, and the change in each biological metric over this trend period (2003-2016).

These data were used for an analysis as part of a long-term project monitoring the biological communities, habitat, and water quality of the Fountain Creek Basin. Biology, habitat, and water quality data have been collected at 10 sites starting in 2003. These data include annual samples of aquatic invertebrate communities, fish communities, water quality, and quantitative riverine habitat collected from 2003-2016. These data will provide insight into trends in biological communities from 2003-2016 and relationships between biological communities and abiotic variables (antecedent streamflow, physical habitat, and water quality). This USGS data release contains all the input and output data and files necessary to reproduce results of the report describing an analysis of ecological trends from 2003-2016 in the Fountain Creek Basin, Colorado, USA (https://doi.org/10.3133/sir20175162). Data preparation of these data files is fully described in the report mentioned previously. These data are outputs from the two-step trend analysis and provide the statistical measures of trend significance, potential drivers of trends, and the change in each biological metric over this trend period (2003-2016).

These data were used for an analysis as part of a long-term project monitoring the biological communities, habitat, and water quality of the Fountain Creek Basin. Biology, habitat, and water quality data have been collected at 10 sites starting in 2003. These data include annual samples of aquatic invertebrate communities, fish communities, water quality, and quantitative riverine habitat collected from 2003-2016. These data will provide insight into trends in biological communities from 2003-2016 and relationships between biological communities and abiotic variables (antecedent streamflow, physical habitat, and water quality).

A data release containing information on benthic macroinvertebrate communities and sediment toxicity in the Eighteenmile Creek Area of Concern and a nearby reference area at Oak Orchard Creek. Bed sediments were collected during summer 2021 at 8 sites on Eighteenmile Creek and at 6 sites on Oak Orchard Creek using a petite Ponar dredge for macroinvertebrate identification and sediment toxicity testing. Macroinvertebrates were identified to the lowest practical taxonomic resolution and used to calculate metrics of biological integrity following standard New York State Department of Environmental Conservation procedures. 10-day sediment toxicity tests were conducted using two test species, Chironomus dilutus and Hyalella azteca, following USEPA test methods 100.2 and 100.1, respectively. In situ habitat measurements and sediment samples for determination of grain size distribution and total organic carbon concentration were also taken at the time of sample collection at all sites. The data release has four separate tables: one containing site locations and habitat information, one containing the results of sediment toxicity tests, one containing macroinvertebrate identifications, and one containing standard New York State Department of Environmental Conservation metrics of macroinvertebrate community integrity calculated from the macroinvertebrate identifications.

A data release containing information on benthic macroinvertebrate communities and sediment toxicity in the Eighteenmile Creek Area of Concern and a nearby reference area at Oak Orchard Creek. Bed sediments were collected during summer 2021 at 8 sites on Eighteenmile Creek and at 6 sites on Oak Orchard Creek using a petite Ponar dredge for macroinvertebrate identification and sediment toxicity testing. Macroinvertebrates were identified to the lowest practical taxonomic resolution and used to calculate metrics of biological integrity following standard New York State Department of Environmental Conservation procedures. 10-day sediment toxicity tests were conducted using two test species, Chironomus dilutus and Hyalella azteca, following USEPA test methods 100.2 and 100.1, respectively. In situ habitat measurements and sediment samples for determination of grain size distribution and total organic carbon concentration were also taken at the time of sample collection at all sites. The data release has four separate tables: one containing site locations and habitat information, one containing the results of sediment toxicity tests, one containing macroinvertebrate identifications, and one containing standard New York State Department of Environmental Conservation metrics of macroinvertebrate community integrity calculated from the macroinvertebrate identifications.