This dataset is from the fourth of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of cobalt (Co), copper (Cu), and nickel (Ni), singly and in mixtures, to natural benthic communities including aquatic insect and algal communities. The methods are the same as those used in Experiment 3, except for the metals combinations. Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, and larvae were collected at the end of the experiment. Additionally, metal residues were measured in sediments, periphyton and Brachycentrus caddisflies (a common, large-bodied, robust taxa that could survive high metals accumulations). Algal biomass responses to metals were measured in situ by in-vivo fluorescence.

This dataset is from the second of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of cadmium (Cd), zinc (Zn), and copper (Cu) singly and in mixtures, to natural aquatic insect communities. Thus, the exposures reproduced those in experiment 1, with the addition of a third metal, Cu Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, while larvae and metal residues in periphyton, sediment, and Brachycentrus caddisflies (a common, large-bodied, robust taxa that could survive high metals accumulations) were collected on the final day of the experiment.

This dataset is from the second of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of cadmium (Cd), zinc (Zn), and copper (Cu) singly and in mixtures, to natural aquatic insect communities. Thus, the exposures reproduced those in experiment 1, with the addition of a third metal, Cu Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, while larvae and metal residues in periphyton, sediment, and Brachycentrus caddisflies (a common, large-bodied, robust taxa that could survive high metals accumulations) were collected on the final day of the experiment.

This dataset is from the third of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of copper (Cu), nickel (Ni), and zinc (Zn), singly and in mixtures, to natural aquatic insect communities. Methods are the same as those in Experiment 2, with the addition of in situ, in-vivo fluorescence measurements of benthic algae. Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, while larvae and metal residues were measured in periphyton and Brachycentrus caddisflies (a common, large-bodied, robust insect that could survive high metals accumulations) at the end of the experiment. In addition, algal biomass was measured in situ by in-vivo fluorescence at the end of the experiment.

This dataset is from the third of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from testing the toxicity of copper (Cu), nickel (Ni), and zinc (Zn), singly and in mixtures, to natural aquatic insect communities. Methods are the same as those in Experiment 2, with the addition of in situ, in-vivo fluorescence measurements of benthic algae. Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions. Emerging adult insects were captured and collected daily throughout the experiment, while larvae and metal residues were measured in periphyton and Brachycentrus caddisflies (a common, large-bodied, robust insect that could survive high metals accumulations) at the end of the experiment. In addition, algal biomass was measured in situ by in-vivo fluorescence at the end of the experiment.

This dataset is from the first of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from an experiment testing the toxicity of cadmium (Cd) and zinc (Zn), singly and in mixtures, to natural aquatic insect communities. Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions.

This dataset is from the first of four experiments which test the toxicity of several metals with aquatic insect communities that were colonized in the field and then transferred and tested in experimental streams. Here we report original data from an experiment testing the toxicity of cadmium (Cd) and zinc (Zn), singly and in mixtures, to natural aquatic insect communities. Trays of cleaned gravel were placed in a stream (the Cache La Poudre River, Colorado) and allowed to colonize for about 40 days before being translocated to Aquatic Experimental Stream Laboratory (AXL) which was located at the USGS Fort Collins Science Center. There the insects were exposed for 30 days to metal mixtures in ratios and concentrations similar to those occurring in ambient conditions.

This data release includes data from laboratory bioassay sediment exposures to Lampsilis siliquoidea and Hyallela azteca. Sediment was collected from streams and rivers within the Tri-State Mining District (TSMD). Endpoints include survival, reproduction and growth, metal concentrations, and various water quality parameters collected during the exposures

This data release includes data from laboratory bioassay water only exposures to Lampsilis siliquoidea. Endpoints include survival and growth, metal concentrations, and various water quality parameters collected during the exposures.

This data release includes data from laboratory bioassay sediment exposures to Lampsilis siliquoidea and Hyallela azteca. Sediment was collected from streams and rivers within the Tri-State Mining District (TSMD). Endpoints include survival, reproduction and growth, metal concentrations, and various water quality parameters collected during the exposures