The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length, shoot number, gemma presence, protonema presence, bound sand mass, and moss organic matter mass were all measured at the end of the experiments. This dataset reports mass adjustment ratios (average non-moss organic matter mass in milligrams per 1 milligram of bound sand mass) that were used to subtract non-moss organic matter mass from total organic matter mass measurements, ultimately calculating moss organic matter mass per fragment at the end of the experiment. Total organic matter mass was determined by placing fragments and attached substrate in a furnace and burning off organic matter. The post-furnace weight was subtracted from the pre-furnace weight to calculate total organic matter mass, with the remaining weight representing the bound sand mass. A separate experiment was performed without moss fragments, but with tackifier and sand, and subjected to the same furnace process to determine the proportion of total organic matter mass made up of sand organic matter and tackifier. Bryum and Syntrichia were grown on different sand types, which were composed of different amounts of organic matter and resulted in different adjustment ratios for Bryum and Syntrichia experiments.

The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length, shoot number, gemma presence, protonema presence, bound sand mass, and moss organic matter mass were all measured at the end of the experiments. This dataset reports all measurements and calculations related to Bryum argenteum and Syntrichia ruralis and additional information describing mass determinations.

The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length, shoot number, gemma presence, protonema presence, bound sand mass, and moss organic matter mass were all measured at the end of the experiments. This dataset reports all measurements and calculations related to Bryum argenteum and Syntrichia ruralis and additional information describing mass determinations.

The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length, shoot number, gemma presence, protonema presence, bound sand mass, and moss organic matter mass were all measured at the end of the experiments. This dataset reports all measurements and calculations related to Syntrichia ruralis.

The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length, shoot number, gemma presence, protonema presence, bound sand mass, and moss organic matter mass were all measured at the end of the experiments. This dataset reports all measurements and calculations related to Syntrichia ruralis.

The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length, shoot number, gemma presence, protonema presence, bound sand mass, and moss organic matter mass were all measured at the end of the experiments. This dataset reports all measurements and calculations related to Bryum argenteum.

The dataset supports a larger study that examined the impacts of three tackifiers (guar, psyllium, and polyacrylamide) on growth of two dryland mosses (Bryum argenteum and Syntrichia ruralis). Moss fragments were grown in petri dishes and subjected to individual tackifiers in one of three possible concentrations (0.5x, 1x, or 2x) of the respective manufacturer's recommended application rate. Distilled water was used as a control treatment, giving a total of ten treatments (nine tackifier-concentration combinations and a water control). Bryum fragments were watered four times daily for six weeks and Syntrichia fragments were watered twice daily for five weeks, after which the experiments were concluded. Shoot length, shoot number, gemma presence, protonema presence, bound sand mass, and moss organic matter mass were all measured at the end of the experiments. This dataset reports all measurements and calculations related to Bryum argenteum.

This dataset contains data supporting the paper: DeCrappeo, N.M., DeLorenze, E.J., Giguere, A.T., Pyke, D.A., and Bottomley, P.J. Fungal and bacterial contributions to nitrogen cycling in cheatgrass-invaded and uninvaded native sagebrush soils of the western USA (accepted at the journal Plant and Soil). The purpose of the study was to evaluate the relative contributions of soil bacteria and fungi to inorganic nitrogen (N) cycling in sagebrush and cheatgrass-invaded soils using a 15N isotope dilution experiment. Soils were collected from sagebrush and cheatgrass rhizospheres at six paired sites in southwest Idaho and southeast Oregon. In order to partition the contribution of each microbial group to N cycling, soils were treated with isotopically labeled N sources and protein synthesis inhibitors. Bronopol and cycloheximide block protein synthesis in bacteria and fungi, respectively; nitrogen can still be taken up by the organisms, but the organisms are unable to assimilate the nutrient into biomass. Laboratory incubations were carried out to study the partitioning of N to microbial biomass and dissolved inorganic nitrogen pools, which were then used to calculate the following nitrogen transformation rates: gross mineralization, net mineralization, ammonium consumption, and net nitrification.

This dataset contains data supporting the paper: DeCrappeo, N.M., DeLorenze, E.J., Giguere, A.T., Pyke, D.A., and Bottomley, P.J. Fungal and bacterial contributions to nitrogen cycling in cheatgrass-invaded and uninvaded native sagebrush soils of the western USA (accepted at the journal Plant and Soil). The purpose of the study was to evaluate the relative contributions of soil bacteria and fungi to inorganic nitrogen (N) cycling in sagebrush and cheatgrass-invaded soils using a 15N isotope dilution experiment. Soils were collected from sagebrush and cheatgrass rhizospheres at six paired sites in southwest Idaho and southeast Oregon. In order to partition the contribution of each microbial group to N cycling, soils were treated with isotopically labeled N sources and protein synthesis inhibitors. Bronopol and cycloheximide block protein synthesis in bacteria and fungi, respectively; nitrogen can still be taken up by the organisms, but the organisms are unable to assimilate the nutrient into biomass. Laboratory incubations were carried out to study the partitioning of N to microbial biomass and dissolved inorganic nitrogen pools, which were then used to calculate the following nitrogen transformation rates: gross mineralization, net mineralization, ammonium consumption, and net nitrification.

This data release contains a formatted dataset compiled from multiple databases on restoration treatments and environmental conditions from across the sagebrush (Artemisia spp.) biome. With these data, we modeled the influence of environmental conditions and restoration treatments on trends in sagebrush cover using generalized additive models. We then used these models to create maps of projected sagebrush cover 30 years following wildfire (no treatment, and aerial or drill seeding of sagebrush). We also provide maps for the probability of recovery after 30 years without treatment, with aerial seeding of sagebrush, or with drill seeding of sagebrush. Widespread degradation of ecosystem function and biodiversity loss has led to calls for massive investments in ecological restoration across the globe, but limited resources necessitate targeted application of restoration efforts. In western North America, disturbances such as wildfire, drought, and invasive species are increasingly altering the sagebrush biome, degrading habitat for species of conservation concern such as greater sage-grouse (Centrocercus urophasianus). Effective restoration is needed to address these challenges, but understanding the conditions determining when, where, and at what rate sagebrush recovery will occur is a pressing research need across the vast and heterogeneous sagebrush landscape. Files included in this data release: sage_dat_release.csv – compiled and formatted multiple treatment and environmental datasets spanning broad spatio-temporal extents sagebrush_notreat.tif – projected sagebrush cover 30 years following wildfire given local environmental conditions, without treatment sagebrush_notreat_sd.tif – error (summarized across simulations) in projected sagebrush cover 30 years following wildfire given local environmental conditions, without treatment perc_change_sage_aerial_artemisia.tif – projected change in sagebrush cover (relative to no treatment) 30 years following wildfire given local environmental conditions, with aerial seeding Artemisia spp. perc_change_sage_aerial_artemisia_sd.tif – error (summarized across simulations) in projected change in sagebrush cover (relative to no treatment) 30 years following wildfire given local environmental conditions, with aerial seeding Artemisia spp. perc_change_sage_drill_artemisia.tif – projected change in sagebrush cover (relative to no treatment) 30 years following wildfire given local environmental conditions, with drill seeding Artemisia spp. perc_change_sage_drill_artemisia_sd.tif – error (summarized across simulations) in projected change in sagebrush cover (relative to no treatment) 30 years following wildfire given local environmental conditions, with drill seeding Artemisia spp. prob_recovery_notreat.tif – probability of recovery 30 years after wildfire, without treatment prob_recovery_aerial_artemisia.tif – probability of recovery 30 years after wildfire, with aerial seeding Artemisia spp. prob_recovery_drill_artemisia.tif – probability of recovery 30 years after wildfire, with drill seeding Artemisia spp.