Data for Figures 2-5. This dataset is associated with the following publication: Shanks, O., A. Diedrich, M. Sivaganesan, J. Willis, and A. Shrifi. Quantitative fecal source characterization of urban municipal storm sewer system outfall ‘wet’ and ‘dry’ weather discharges. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 259: 121857, (2024).

In cooperation with the New York City Department of Environmental Protection (NYC DEP), the U.S. Geological Survey (USGS) collected water and bed sediment samples along Alley Creek (Queens, New York) to help determine likely sources of fecal bacteria to the creek and Little Neck Bay. Potential terrestrial sources include stormwater, sewage via combined sewer overflow (CSO) and compromised infrastructure, bed-sediment resuspension, and groundwater discharge. Host sources that were targeted using microbial source tracking (MST) techniques included human, canine, waterfowl, and general Bacteroides. Routine water samples were collected and analyzed for the fecal indicator bacteria enterococci and fecal coliform, along with the physicochemical constituent total suspended solids (TSS), at the NYC DEP Newton Creek Microbiological Laboratory (Brooklyn, New York) and for MST markers at the USGS Ohio Water Microbiological Laboratory (OWML; Columbus, Ohio)--these results from routine samples are available at the USGS National Water Information System (NWIS) Web interface (https://waterdata.usgs.gov/nwis/qw). Data in this data release were generated from the two local investigations of NYC DEP sites TI-008 and TI-24 collected to supplement routine monitoring. At TI-008, sediment and water samples were collected along a stormwater conveyance line between Oakland Lake and Alley Creek on September 22, 2020 to conduct a sediment resuspension experiment. In May 2021, a total of 20 surface water and depth-profile samples (taken from a hole in a 7-foot submerged wastewater pipe adjacent to TI-024), along with thermal imagery, were collected adjacent to combined sewer overflow outfall TI-024.

In cooperation with the New York City Department of Environmental Protection (NYC DEP), the U.S. Geological Survey (USGS) collected water and bed sediment samples along Alley Creek (Queens, New York) to help determine likely sources of fecal bacteria to the creek and Little Neck Bay. Potential terrestrial sources include stormwater, sewage via combined sewer overflow (CSO) and compromised infrastructure, bed-sediment resuspension, and groundwater discharge. Host sources that were targeted using microbial source tracking (MST) techniques included human, canine, waterfowl, and general Bacteroides. Routine water samples were collected and analyzed for the fecal indicator bacteria enterococci and fecal coliform, along with the physicochemical constituent total suspended solids (TSS), at the NYC DEP Newton Creek Microbiological Laboratory (Brooklyn, New York) and for MST markers at the USGS Ohio Water Microbiological Laboratory (OWML; Columbus, Ohio)--these results from routine samples are available at the USGS National Water Information System (NWIS) Web interface (https://waterdata.usgs.gov/nwis/qw). Data in this data release were generated from the two local investigations of NYC DEP sites TI-008 and TI-24 collected to supplement routine monitoring. At TI-008, sediment and water samples were collected along a stormwater conveyance line between Oakland Lake and Alley Creek on September 22, 2020 to conduct a sediment resuspension experiment. In May 2021, a total of 20 surface water and depth-profile samples (taken from a hole in a 7-foot submerged wastewater pipe adjacent to TI-024), along with thermal imagery, were collected adjacent to combined sewer overflow outfall TI-024.

Data set includes measurements used in Figure 2.

Fecal source identification qPCR, land use, and weather data used in the manuscript entitled, "Large-Scale Implementation of Standardized Quantitative Real-Time PCR Fecal Source Identification Procedures in the Tillamook Bay Watershed". This dataset is associated with the following publication: Li, X., M. Sivaganesan, C. Kelty, A. Zimmer-Faust, P. Clinton, J. Reichman, Y. Johnson, W. Matthews, S. Bailey, and O. Shanks. Large-scale implementation of standardized quantitative real-time PCR fecal source identification procedures in the Tillamook Bay Watershed. PLoS ONE. Public Library of Science, San Francisco, CA, USA, 14(6): e0216827, (2019).

Estimated mean log10 concentration of genetic markers for CQQ_056, CPQ_064, HF183/BacR287, and HumM2 genetic markers in nine sewage and two surface water samples. This dataset is associated with the following publication: Stachler, E., C. Kelty, M. Sivaganesan, X. Li, K. Bibby, and O. Shanks. Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 51(16): 9146-9154, (2017).

Estimated mean log10 concentration of genetic markers for CQQ_056, CPQ_064, HF183/BacR287, and HumM2 genetic markers in nine sewage and two surface water samples. This dataset is associated with the following publication: Stachler, E., C. Kelty, M. Sivaganesan, X. Li, K. Bibby, and O. Shanks. Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 51(16): 9146-9154, (2017).

Nutrients, rainfall, E coli, water quality parameters. This dataset is associated with the following publication: Friedman, S., E. Cooper, A. Blackwell, M.A. Elliott, M. Weinstein, J. Cara, and Y. Wan. A multi-tiered approach to assess fecal pollution in an urban watershed: Bacterial and viral indicators and sediment microbial communities. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 945: 174141, (2024).

Data contains information about bacterial concentrations in the water column in a mixed use watershed in Northeast GA. This dataset is associated with the following publication: Sowah, R., K. Bradshaw, B. Snyder, D. Spidle, and M. Molina. Evaluation of the soil and water assessment tool (SWAT) for simulating E. coli concentrations at the watershed-scale. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 746: 140669, (2020).

Dataset describes measurements of host-associated qPCR genetic markers along with other water quality parameters and precipitation from samples collected at marine, estuary, and freshwater recreational sites. Additional details provided in attached Dataset Description document. “This research dataset has been reviewed in accordance with U.S. Environmental Protection Agency (U.S. EPA), Office of Research and Development, and approved for release. Mention of brand names or vendors does not constitute an endorsement of products or services by the U.S. EPA.”