This study evaluated the use of dissolved carbon dioxide as a control method for juvenile red swamp crayfish (Procambarus clarkii). Four concentrations of CO2 and a control were administered to 18 test tanks over a 96-hour period. Red swamp crayfish were observed for behavioral changes during the exposure and were assessed for mortality 24 hours post treatment. Datasets include daily care, water quality, biometric, behavioral observation, flow rate, and CO2 titration data from one trial. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

This data was collected to determine responses of red swamp crayfish and rusty crayfish to CO2-enriched water to determine the utility of CO2 as a crayfish control tool. Avoidance tests, emergence tests, and loss of equilibrium (LOE) tests were performed to determine crayfish behavior in CO2-enriched water. Avoidance data (TimeinChamber2.csv, Crossings.csv, FirstLastShuttle.csv, AvoidanceLengthsandWeights.csv) were collected on 20171220 through 20180214. CO2 was injected in one side of a choice chamber under light or dark conditions and 10oC or 24oC water temperature. Shuttle movements were recorded and used to determine the number of crossings from the CO2 injected side to the non-injected side, the CO2 concentration and time of the first and last shuttle after CO2 injection began, and the total times spent in the injected and non-injected chambers. Loss of equilibrium (LOE) data were collected on 20180117 through 20180119 (LOEStats.csv). Crayfish were placed in a bucket with water and CO2 was injected into the bucket until the crayfish lost equilibrium. The concentration of CO2 and time until LOE occurred was recorded along with other behaviors induced by the elevated CO2 concentration. Red swamp crayfish emergence data was collected on 20180221 through 20180227 (Incline.csv). One side of the choice chamber used in the behavior/shuttle trials was isolated and an incline ramp was placed on the tank. CO2 was then injected into the tank and the behavior of the crayfish was recorded. Behavior data was recorded as in the tank, on the incline, or out of the water.

This data was collected to determine responses of red swamp crayfish and rusty crayfish to CO2-enriched water to determine the utility of CO2 as a crayfish control tool. Avoidance tests, emergence tests, and loss of equilibrium (LOE) tests were performed to determine crayfish behavior in CO2-enriched water. Avoidance data (TimeinChamber2.csv, Crossings.csv, FirstLastShuttle.csv, AvoidanceLengthsandWeights.csv) were collected on 20171220 through 20180214. CO2 was injected in one side of a choice chamber under light or dark conditions and 10oC or 24oC water temperature. Shuttle movements were recorded and used to determine the number of crossings from the CO2 injected side to the non-injected side, the CO2 concentration and time of the first and last shuttle after CO2 injection began, and the total times spent in the injected and non-injected chambers. Loss of equilibrium (LOE) data were collected on 20180117 through 20180119 (LOEStats.csv). Crayfish were placed in a bucket with water and CO2 was injected into the bucket until the crayfish lost equilibrium. The concentration of CO2 and time until LOE occurred was recorded along with other behaviors induced by the elevated CO2 concentration. Red swamp crayfish emergence data was collected on 20180221 through 20180227 (Incline.csv). One side of the choice chamber used in the behavior/shuttle trials was isolated and an incline ramp was placed on the tank. CO2 was then injected into the tank and the behavior of the crayfish was recorded. Behavior data was recorded as in the tank, on the incline, or out of the water.

This study evaluated carbon dioxide (CO2) injected into water as a possible behavioral stimulant to enhance capture and removal of invasive red swamp crayfish (RSC, Procambarus clarkii Girard, 1852) from a retention pond in southeastern Michigan. Objectives of this study were to (1) determine if target CO2 concentrations were attainable within the infested pond, and (2) determine if CO2 treatment was effective to push RSC towards shorelines or onto dry land where they could be collected and removed. Carbon dioxide was applied directly into one treatment pond (~2,500 m3) in Novi, MI. Two nearby ponds in Livonia, MI were used as untreated control ponds. Crayfish removal efficiency was evaluated in all ponds using baited traps and shoreline surveys. Results showed that the CO2 treatment pond reached its target concentration of >200 milligrams per liter (mg/L) CO2, a benchmark determined from previous laboratory studies, approximately 11 hours after injection started and was maintained between 200-351 mg/L CO2 for approximately 2.5 days. During treatment, some emergent crayfish were observed near influent culverts around the pond, possibly indicative of a behavioral response. However, the number of individuals and crayfish observations were minimal and infrequent. Crayfish continued to be removed throughout CO2 treatment with baited traps and perimeter surveys, but differences in catch rates between the treatment and control ponds were not apparent and confounded by a temporal decline in catch rates across all ponds. Overall, this study demonstrated that open-water treatment applications with CO2 are possible, but its effectiveness to enhance RSC removal was unclear due to limited crayfish observations.

This study evaluated carbon dioxide (CO2) injected into water as a possible behavioral stimulant to enhance capture and removal of invasive red swamp crayfish (RSC, Procambarus clarkii Girard, 1852) from a retention pond in southeastern Michigan. Objectives of this study were to (1) determine if target CO2 concentrations were attainable within the infested pond, and (2) determine if CO2 treatment was effective to push RSC towards shorelines or onto dry land where they could be collected and removed. Carbon dioxide was applied directly into one treatment pond (~2,500 m3) in Novi, MI. Two nearby ponds in Livonia, MI were used as untreated control ponds. Crayfish removal efficiency was evaluated in all ponds using baited traps and shoreline surveys. Results showed that the CO2 treatment pond reached its target concentration of >200 milligrams per liter (mg/L) CO2, a benchmark determined from previous laboratory studies, approximately 11 hours after injection started and was maintained between 200-351 mg/L CO2 for approximately 2.5 days. During treatment, some emergent crayfish were observed near influent culverts around the pond, possibly indicative of a behavioral response. However, the number of individuals and crayfish observations were minimal and infrequent. Crayfish continued to be removed throughout CO2 treatment with baited traps and perimeter surveys, but differences in catch rates between the treatment and control ponds were not apparent and confounded by a temporal decline in catch rates across all ponds. Overall, this study demonstrated that open-water treatment applications with CO2 are possible, but its effectiveness to enhance RSC removal was unclear due to limited crayfish observations.

Data were collected associated with the application of a pesticide to a stormwater retention pond and burrows to suppress or eradicate an invasive crayfish species, Procambarus clarkii, in support of high-priority research developing control methods to mitigate impacts of invasive crayfish within the Great Lakes Basin. Effectiveness of the treatment was accessed using an in-situ bioassay and by measuring pesticide concentrations in water, sediment, and caged crayfish. Water quality data, including temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, and turbidity, in stormwater ponds was collected to evaluate whether environmental conditions may impact treatment effectiveness and persistence of pesticide. Pesticide concentrations were assessed prior to chemical application of ponds and monitored for 88 days post application. Pesticide concentrations in burrows and adjacent pond were monitored prior to treating burrows and for up to three days post application. Research will assist management and regulatory agencies in interpretating laboratory acute and chronic data relative to field-based treatment and effects data, and in developing permitting requirements and best management practices for open-water and burrowed invasive crayfish populations. Data associated with laboratory studies were collected to determine 24-hr acute lethal concentrations of two pesticides containing pyrethrin or cypermethrin for two crayfish species, Procambarus clarkii and Faxonius virilis. Tests investigated whether two nominal temperatures (10 and 22 °C) and total suspended solid affected pesticide toxicity. Water quality monitoring during testing followed standard testing protocols and included temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, turbidity, and total suspended solids in test jars with sediment from West Bearskin Lake, MN (about 2% Total Organic Carbon (TOC)). Water was collected from water treatments with and without sediment for pesticide concentrations. Pesticide concentrations are reported for water and for filters plus sediment. Test organisms were exposed to pesticides for 24-hr before being assessed for survival.

Data were collected associated with the application of a pesticide to a stormwater retention pond and burrows to suppress or eradicate an invasive crayfish species, Procambarus clarkii, in support of high-priority research developing control methods to mitigate impacts of invasive crayfish within the Great Lakes Basin. Effectiveness of the treatment was accessed using an in-situ bioassay and by measuring pesticide concentrations in water, sediment, and caged crayfish. Water quality data, including temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, and turbidity, in stormwater ponds was collected to evaluate whether environmental conditions may impact treatment effectiveness and persistence of pesticide. Pesticide concentrations were assessed prior to chemical application of ponds and monitored for 88 days post application. Pesticide concentrations in burrows and adjacent pond were monitored prior to treating burrows and for up to three days post application. Research will assist management and regulatory agencies in interpretating laboratory acute and chronic data relative to field-based treatment and effects data, and in developing permitting requirements and best management practices for open-water and burrowed invasive crayfish populations. Data associated with laboratory studies were collected to determine 24-hr acute lethal concentrations of two pesticides containing pyrethrin or cypermethrin for two crayfish species, Procambarus clarkii and Faxonius virilis. Tests investigated whether two nominal temperatures (10 and 22 °C) and total suspended solid affected pesticide toxicity. Water quality monitoring during testing followed standard testing protocols and included temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, turbidity, and total suspended solids in test jars with sediment from West Bearskin Lake, MN (about 2% Total Organic Carbon (TOC)). Water was collected from water treatments with and without sediment for pesticide concentrations. Pesticide concentrations are reported for water and for filters plus sediment. Test organisms were exposed to pesticides for 24-hr before being assessed for survival.

In order to estimate the costs of chemical control of non-indigenous crayfish versus the cost of manual inspection of fish hatchery shipments a cost analysis was performed. The data includes ranking the likelihood of missing non-indigenous crayfish and effects on fish of handling during manual searches and the effects on fish during chemical exposure. The data also includes estimates of the time, staff, and salary requirements and cost of materials for each approach.

Invasive crayfish are known to displace native crayfish species, alter aquatic habitat and community structure and function, and are serious pests for fish hatcheries. White River Crawfish (WRC; Procambarus acutus) were inadvertently introduced to a warm-water fish hatchery in Missouri, USA, possibly in an incoming fish shipment. We evaluated the use of chemical control for crayfish to ensure incoming and outgoing fish shipments from hatcheries do not contain live crayfish. We conducted acute (less than or equal to 24 hr) static toxicity tests to determine potency, dose-response, and selectivity of pesticides to WRC, Virile Crayfish (VC; Orconectes virilis), and Fathead Minnow (FHM; Pimephales promelas). Data included are: Collection location and size of test organisms; Test chemical concentrations and recovery; Mortality and effect-based responses of test organisms; Water quality of test solutions

These data consist of three related tables describing test conditions including pesticide concentration, water quality, and post-treatment survival of crayfish associated with laboratory studies conducted to determine 24-hr acute lethal concentrations of two pesticides containing pyrethrin or cypermethrin for two crayfish species, Procambarus clarkii and Faxonius virilis. Tests investigated whether two nominal temperatures (10 and 22 °C) and total suspended solids in water affected pesticide toxicity. Laboratory data will be used to design field treatments for open-water populations of invasive P. clarkii. Water quality monitoring during testing followed standard testing protocols and included temperature, pH, specific conductance, dissolved oxygen, alkalinity, hardness, ammonia, turbidity, and total suspended solids in test jars with sediment from West Bearskin Lake, MN (about 2% Total Organic Carbon (TOC)). Water samples were collected from water treatments with and without sediment for pesticide concentrations. Pesticide concentrations are reported for water and for filters plus sediment. Test organisms were exposed to pesticides for 24-hr prior before being assessed for survival.