Biological indicators including morphometric (length, weight), age, sex and health indicators including organismal (health assessment index, condition factor), organ (hepatosomatic index, gonadosomatic index), cellular (intersex, parasite density, macrophage aggregate density), subcellular (plasma vitellogenin, estradiol, testosterone, 11-keto testosterone) and molecular (hepatic transcript abundance) were measured in smallmouth bass from tributaries in the Chesapeake Bay watershed. Total mercury was measured in these fish and correlations between mercury and biological indicators were assessed.

Comma-separated values (.csv) file containing data related to mercury in smallmouth bass (Micropterus dolomieu) collected from the Snake River, USA.

Comma-separated values (.csv) file containing data related to mercury in smallmouth bass (Micropterus dolomieu) collected from the Snake River, USA.

Comma-separated values (.csv) file containing data related to mercury in smallmouth bass (Micropterus dolomieu) collected from the Snake River, USA.

This dataset includes fish scientific name, fish common name, site identification codes, latitude, longitude, site name, sample identification codes, collection date, tissue type, percent moisture content in fish samples, and wet and dry total mercury concentrations in fish tissues.

This dataset includes fish scientific name, fish common name, site identification codes, latitude, longitude, site name, sample identification codes, collection date, tissue type, percent moisture content in fish samples, and wet and dry total mercury concentrations in fish tissues.

Comma-separated values (.csv) files containing data related to amphibian, fish, and sediment sampling near Churchill, Manitoba between 2015-2019. Data files contain mercury concentrations in amphibian and fish tissues, amphibian egg masses, and sediment, as well as amphibian and fish morphometrics, life stage, site distance to coast and biome where the samples were collected.

Comma-separated values (.csv) files containing data related to amphibian, fish, and sediment sampling near Churchill, Manitoba between 2015-2019. Data files contain mercury concentrations in amphibian and fish tissues, amphibian egg masses, and sediment, as well as amphibian and fish morphometrics, life stage, site distance to coast and biome where the samples were collected.

Adult and young of the year smallmouth bass from the Chesapeake Bay watershed have faced health issues (mortality events, disease by a variety of pathogens, endocrine disruption in male fish, evidence of oxidative damage to tissues, and signs of exposure to chemical contaminants) at multiple sites within the Susquehanna and Potomac River basins. Functional immune assays were added to long-term comprehensive monitoring assessments to determine if immunosuppression was playing a role in observed disease and mortalities because no one single or consistent cause could be identified. The functional immune assays measured bactericidal activity, respiratory burst activity, and lymphocyte mitogenesis using leukocytes isolated from anterior kidney tissue. From 2016-2021, a total of 740 smallmouth bass were sampled among eight (8) sites. Twenty adults were sampled from each site in spring (pre-spawn; April – May) and fall (recrudescence; October – November) at different points in the study. Ancillary data, including biometrics of the fish and parasite/macrophage aggregate counts in multiple tissue types, were collected in conjunction with the immune data. The functional immune assays serve as part of an integrated assessment to determine environmental stressors and infectious agents that may modulate the immune response in wild smallmouth bass, ultimately making them more susceptible to disease and death. Therefore, it is important to consider the functional immune data alongside other aspects of host and environmental health.

Adult and young of the year smallmouth bass from the Chesapeake Bay watershed have faced health issues (mortality events, disease by a variety of pathogens, endocrine disruption in male fish, evidence of oxidative damage to tissues, and signs of exposure to chemical contaminants) at multiple sites within the Susquehanna and Potomac River basins. Functional immune assays were added to long-term comprehensive monitoring assessments to determine if immunosuppression was playing a role in observed disease and mortalities because no one single or consistent cause could be identified. The functional immune assays measured bactericidal activity, respiratory burst activity, and lymphocyte mitogenesis using leukocytes isolated from anterior kidney tissue. From 2016-2021, a total of 740 smallmouth bass were sampled among eight (8) sites. Twenty adults were sampled from each site in spring (pre-spawn; April – May) and fall (recrudescence; October – November) at different points in the study. Ancillary data, including biometrics of the fish and parasite/macrophage aggregate counts in multiple tissue types, were collected in conjunction with the immune data. The functional immune assays serve as part of an integrated assessment to determine environmental stressors and infectious agents that may modulate the immune response in wild smallmouth bass, ultimately making them more susceptible to disease and death. Therefore, it is important to consider the functional immune data alongside other aspects of host and environmental health.