1. 16S rRNA sequences, generated using Illumina sequencer, and 2. water parameters, included temperature and chlorine residual concentrations. This dataset is associated with the following publication: Zhang, C., K. Qin, I. Struewing, H.Y. Buse, J. SantoDomingo, D. Lytle, and J. Lu. The Bacterial Community Diversity of Bathroom Hot Tap Water Was Significantly Lower Than That of Cold Tap and Shower Water. Frontiers in Microbiology. Frontiers, Lausanne, SWITZERLAND, 12: 625324, (2021).

This is a compiled data set for the qPCR analyses, bacterial enumeration analyses, and water quality parameters collected for this study. This dataset is associated with the following publication: Buse, H., B. Morris, and E. Rice. Early detection of viable Francisella tularensis in environmental matrices byculture-based PCR. BMC Microbiology. BioMed Central Ltd, London, UK, 20(66): 15, (2020).

This is a compiled data set for the qPCR analyses, bacterial enumeration analyses, and water quality parameters collected for this study. This dataset is associated with the following publication: Buse, H., B. Morris, and E. Rice. Early detection of viable Francisella tularensis in environmental matrices byculture-based PCR. BMC Microbiology. BioMed Central Ltd, London, UK, 20(66): 15, (2020).

Estimated mean log10 concentration of genetic markers for CQQ_056, CPQ_064, HF183/BacR287, and HumM2 genetic markers in nine sewage and two surface water samples. This dataset is associated with the following publication: Stachler, E., C. Kelty, M. Sivaganesan, X. Li, K. Bibby, and O. Shanks. Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 51(16): 9146-9154, (2017).

Estimated mean log10 concentration of genetic markers for CQQ_056, CPQ_064, HF183/BacR287, and HumM2 genetic markers in nine sewage and two surface water samples. This dataset is associated with the following publication: Stachler, E., C. Kelty, M. Sivaganesan, X. Li, K. Bibby, and O. Shanks. Quantitative CrAssphage PCR Assays for Human Fecal Pollution Measurement. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 51(16): 9146-9154, (2017).

This U.S. Geological Survey (USGS) Data Release provides water-quality data collected during 1-week and 2-week nutrient studies beginning on February 22, May 17, and July 26 located in the Caloosahatchee River at the S-79 water-control structure. For each study period, 12 fiberglass tanks were suspended in the river using three floating cradles with each cradle holding four tanks. Each tank was open to the atmosphere and closed to the river. The tanks were filled with native water during the first day of each study period. Four different treatment methods were represented within each cradle. For all three study periods, two of the four treatments remained constant: ammonium hydroxide and untreated. For the 1-week study period in February, the additional treatment methods were sodium phosphate dibasic dodecahydrate and sodium nitrate. For the 2-week study period in May, the additional treatment methods were sodium phosphate dibasic dodecahydrate and urea. For the 2-week study period during July-August, the additional treatment methods were sodium nitrate and urea. Treatments were added to the tanks following the profiles on the first day of each study period. Nutrient samples were collected and processed by Nova Southeastern University. Water-quality sensor data were collected near the surface (approximately 1 foot below the water surface), near the middle of the water column (approximately 2 feet below the water surface), and near the bottom (approximately 3 feet below the water surface) of each tank using a multi-parameter water-quality sonde. Additional water-quality sensor data were collected at approximately 1, 2, and 3 feet below the water surface within the river. Each point reading is provided as an instantaneous measurement. Water-quality parameters measured include water temperature, specific conductance, pH, dissolved oxygen, turbidity, phycocyanin fluorescence, chlorophyll fluorescence, and fluorescence of dissolved organic matter. Revision History: First release: November 2023. Version 1.1: August 2024. Changes from previous version: in file "Mesocosms_profiles_2021_datarelease_data" revised one of the four treatment methods for the July-August study period from sodium phosphate dibasic dodecahydrate to sodium nitrate to correct an Excel entry error.

The dataset includes water-quality sensor readings collected by the U.S Geological Survey (USGS) from the Caloosahatchee River at the Franklin Lock and Dam and 12 open-air fiberglass tanks filled with Caloosahatchee River water used for mesocosm experiments testing the effects of elevated nutrients on harmful algal bloom (HAB) dynamics. This dataset contains water quality sensor readings from two of eight total independent experiments conducted from June 8-11, 2020 and September 14-17, 2020. Each of the 12 tanks were randomly treated with either ammonium hydroxide, sodium nitrate, sodium phosphate, or left untreated (controls) for a total of three replicates of each treatment. The tanks were treated with incrementally higher dosing solutions every 24 hours for the first three days of the four-day experiment (T0, T24, and T48). Biological and nutrient concentration samples were collected each day before and after the dosing solution was applied and at T72, the final day of the experiment. Water-quality sensor data were collected on all four days (T0, T24, T48, T72) at three depths within each tank and the river using a multi-parameter water-quality sonde before the dosing solutions were applied. Each point reading is provided as an instantaneous measurement. Water-quality parameters measured include chlorophyll fluorescence, dissolved oxygen, fluorescence of dissolved organic matter, pH, phycocyanin fluorescence, specific conductance, turbidity, and water temperature. The data is provided as a table in comma delimited format.

This U.S. Geological Survey (USGS) Data Release provides water-quality data collected during 1-week and 2-week nutrient studies beginning on February 22, May 17, and July 26 located in the Caloosahatchee River at the S-79 water-control structure. For each study period, 12 fiberglass tanks were suspended in the river using three floating cradles with each cradle holding four tanks. Each tank was open to the atmosphere and closed to the river. The tanks were filled with native water during the first day of each study period. Four different treatment methods were represented within each cradle. For all three study periods, two of the four treatments remained constant: ammonium hydroxide and untreated. For the 1-week study period in February, the additional treatment methods were sodium phosphate dibasic dodecahydrate and sodium nitrate. For the 2-week study period in May, the additional treatment methods were sodium phosphate dibasic dodecahydrate and urea. For the 2-week study period during July-August, the additional treatment methods were sodium nitrate and urea. Treatments were added to the tanks following the profiles on the first day of each study period. Nutrient samples were collected and processed by Nova Southeastern University. Water-quality sensor data were collected near the surface (approximately 1 foot below the water surface), near the middle of the water column (approximately 2 feet below the water surface), and near the bottom (approximately 3 feet below the water surface) of each tank using a multi-parameter water-quality sonde. Additional water-quality sensor data were collected at approximately 1, 2, and 3 feet below the water surface within the river. Each point reading is provided as an instantaneous measurement. Water-quality parameters measured include water temperature, specific conductance, pH, dissolved oxygen, turbidity, phycocyanin fluorescence, chlorophyll fluorescence, and fluorescence of dissolved organic matter. Revision History: First release: November 2023. Version 1.1: August 2024. Changes from previous version: in file "Mesocosms_profiles_2021_datarelease_data" revised one of the four treatment methods for the July-August study period from sodium phosphate dibasic dodecahydrate to sodium nitrate to correct an Excel entry error.

They are qPCR data for opportunistic pathogens and drinking water parameters. This dataset is associated with the following publication: Zhang, C., I. Struewing, J. Mistry, D. Wahman, J. Pressman, and J. Lu. Legionella and other opportunistic pathogens in full-scale chloraminated municipal drinking water distribution systems. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 205: 117571, (2021).

Water-quality data were collected by Providence Water, formerly referred to as Providence Water Supply Board (PWSB), from tributaries in the Scituate Reservoir drainage area, October 1, 2017 through September 30, 2024 (water years 2018 through 2024). Water-quality samples were collected by the PWSB either monthly or quarterly at fixed stations on 38 tributaries in the Scituate Reservoir drainage area, Rhode Island. These data were used to calculate instantaneous loads and yields of constituents in reports by the U.S. Geological Survey. Water-quality samples were collected following a strict sampling schedule so that water-quality samples would be representative of various weather conditions. Samples were analyzed at the PWSB water-quality laboratory at the P.J. Holton Water Purification Plant in Scituate, Rhode Island. Water-quality properties and constituent concentrations were measured by using unfiltered water samples. These water-quality properties included pH, alkalinity, color, turbidity, and concentrations of chloride, nitrite, nitrate, orthophosphate, and bacteria (Escherichia coli [E. coli] and total coliform). This data release will be updated to append additional water-quality data collected by the PWSB. First posted May 2021, ver. 1.0 Revised July 2022, ver. 2.0 Revised November 2023, ver. 3.0 Revised December 2025, ver. 4.0