The data set involved mice consumed diets amended with a lead-contaminated soil in its native (untreated) state or after treatment with phosphoric acid or triple superphosphate alone or in combination with iron-waste material or biosolids compost. This dataset is associated with the following publication: George, S., J. James, R. Devereux, Y. Wan, G. Diamond, K. Bradham, K. Scheckel, and D. Thomas. Ingestion of Remediated Lead-Contaminated Soils Affects the Fecal Microbiome of Mice. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 837: 155797, (2022).

The effect of dietary lead (Pb) on the murine intestinal microbiome was explored to determine if there was an association between microflora modulation and Pb source. Mice were treated with 6.25-25 ppm Pb acetate (PbOAc) or 7.5-30 ppm Pb in reference soil SRM 2710a having 0.552% Pb among other heavy metals such as Cd. Feces and ceca were collected following 9 days of treatment and the microbiome analyzed by 16S rRNA gene sequencing. Treatment effects on the microbiome were observed in both feces and ceca of mice. Changes in the cecal microbiomes of mice fed Pb were similar except for a few exceptions regardless of source. Akkermansia, a common gut bacterium, was highest ranked species in control microbiomes whereas Lactobacillus ranked highest in treated mice. Firmicutes/Bacteroidetes ratios in the ceca of PbOAc and SRM 2710a treated mice increased suggestive of changes in gut microbiome metabolism that promotes obesity. Bacilli/Clostridia increased in the ceca of PbOAc treated mice and may be indicative of increased risk of host sepsis. Family Deferribacteraceae also were modulated by PbOAc or SRM 2710a, respectively, possibly impacting iron metabolism. Understanding the relationship between microbiome composition and Pb concentration, especially in soil, may ultimately provide new insights into the utility of various remediation methodologies that modulate the effects and assist in the selection of an optimal treatment for a specific contaminated site. This dataset is associated with the following publication: George, S., R. Devereux, J. James, Y. Wan, G. Diamond, K. Bradham, and D. Thomas. Dietary lead modulates the mouse intestinal microbiome: Subacute exposure to lead acetate and lead contaminated soil. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY. Elsevier Science Ltd, New York, NY, USA, 249: (2023).

The effect of dietary lead (Pb) on the murine intestinal microbiome was explored to determine if there was an association between microflora modulation and Pb source. Mice were treated with 6.25-25 ppm Pb acetate (PbOAc) or 7.5-30 ppm Pb in reference soil SRM 2710a having 0.552% Pb among other heavy metals such as Cd. Feces and ceca were collected following 9 days of treatment and the microbiome analyzed by 16S rRNA gene sequencing. Treatment effects on the microbiome were observed in both feces and ceca of mice. Changes in the cecal microbiomes of mice fed Pb were similar except for a few exceptions regardless of source. Akkermansia, a common gut bacterium, was highest ranked species in control microbiomes whereas Lactobacillus ranked highest in treated mice. Firmicutes/Bacteroidetes ratios in the ceca of PbOAc and SRM 2710a treated mice increased suggestive of changes in gut microbiome metabolism that promotes obesity. Bacilli/Clostridia increased in the ceca of PbOAc treated mice and may be indicative of increased risk of host sepsis. Family Deferribacteraceae also were modulated by PbOAc or SRM 2710a, respectively, possibly impacting iron metabolism. Understanding the relationship between microbiome composition and Pb concentration, especially in soil, may ultimately provide new insights into the utility of various remediation methodologies that modulate the effects and assist in the selection of an optimal treatment for a specific contaminated site. This dataset is associated with the following publication: George, S., R. Devereux, J. James, Y. Wan, G. Diamond, K. Bradham, and D. Thomas. Dietary lead modulates the mouse intestinal microbiome: Subacute exposure to lead acetate and lead contaminated soil. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY. Elsevier Science Ltd, New York, NY, USA, 249: (2023).

The files in the dataset include tables of Pb bioavailability data from mice studies, of Pb x-ray absorption near-edge structure spectroscopy data, and of Pb speciation results of diets and excreted Pb. This dataset is associated with the following publication: Karna, R.R., M.P. Noerpel, C. Nelson, B. Elek, K. Herbin-Davis, G. Diamond , K. Bradham, D.J. Thomas, and K.G. Scheckel. Bioavailable soil Pb minimized by in situ transformation to plumbojarosite. PNAS (PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES). National Academy of Sciences, WASHINGTON, DC, USA, 118(3): 01-06, (2021).

The files in the dataset include tables of Pb bioavailability data from mice studies, of Pb x-ray absorption near-edge structure spectroscopy data, and of Pb speciation results of diets and excreted Pb. This dataset is associated with the following publication: Karna, R.R., M.P. Noerpel, C. Nelson, B. Elek, K. Herbin-Davis, G. Diamond , K. Bradham, D.J. Thomas, and K.G. Scheckel. Bioavailable soil Pb minimized by in situ transformation to plumbojarosite. PNAS (PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES). National Academy of Sciences, WASHINGTON, DC, USA, 118(3): 01-06, (2021).

The data set comprises of two tables the show the linear combination fitting results for lead and iron speciation in samples collected for the study. The tables show the names of samples pre- and post-treatment and the relative abundance of various lead and iron species present in each sample. This dataset is associated with the following publication: Kastury, F., E. Smith, E. Doelsh, E. Lombi, M. Donnelley, P. Cmielewski, D.W. Parsons, K.G. Scheckel, D. Paterson, M.D. de Jonge, C. Herde, and A.L. Juhasz. In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments.. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 53(17): 10329-10341, (2019).

The data set comprises of two tables the show the linear combination fitting results for lead and iron speciation in samples collected for the study. The tables show the names of samples pre- and post-treatment and the relative abundance of various lead and iron species present in each sample. This dataset is associated with the following publication: Kastury, F., E. Smith, E. Doelsh, E. Lombi, M. Donnelley, P. Cmielewski, D.W. Parsons, K.G. Scheckel, D. Paterson, M.D. de Jonge, C. Herde, and A.L. Juhasz. In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments.. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 53(17): 10329-10341, (2019).

Linear combination fitting data for lead speciation of soil samples evaluated through an in-vivo/in-vitro correlation for quail exposure. This dataset is associated with the following publication: Beyer, W.N., N. Basta, R. Chaney, P. Henry, D. Mosby, B. Rattner, K. Scheckel , D. Sprague, and J. Weber. BIOACCESSIBILITY TESTS ACCURATELY ESTIMATE BIOAVAILABILITY OF LEAD TO QUAIL. G.A. Burton, Jr., and C. H. Ward ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 35(9): 2311-2319, (2016).

Linear combination fitting data for lead speciation of soil samples evaluated through an in-vivo/in-vitro correlation for quail exposure. This dataset is associated with the following publication: Beyer, W.N., N. Basta, R. Chaney, P. Henry, D. Mosby, B. Rattner, K. Scheckel , D. Sprague, and J. Weber. BIOACCESSIBILITY TESTS ACCURATELY ESTIMATE BIOAVAILABILITY OF LEAD TO QUAIL. G.A. Burton, Jr., and C. H. Ward ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 35(9): 2311-2319, (2016).

These data were collected by the EPA Toxic Element Bioavailability Laboratory and consists of lead (Pb) and/or arsenic (As) mouse tissue, soil, aqueous soil leachate, and spectroscopy data. The mouse-based assay for assessment of lead and arsenic in vivo mouse bioavailability in soil and dust is a critical tool needed to improve our understanding of the factors affecting the metal’s uptake across the gastrointestinal barrier and to evaluate the efficacy of methods used to remediate lead and/or arsenic contaminated media. The mouse assay has been optimized to yield accurate, reproducible, and reliable estimates of the in vivo mouse bioavailability of lead and/or arsenic in soil and dust. The reported Pb/As data is used to assess the total, bioaccessible, and bioavailable concentrations resulting from exposure to soils. These data were collected using EPA Methods 1340, 3050b, 6010d, and 6020b. These quantitative characterization data are then paired with X-ray absorption spectroscopy data that is used to deduce the valence, coordination environment, and mineral/sorbed phase of the element of interest. These data are collected at a synchrotron facility by our team and then processed at EPA ORD using previously established methods (e.g., Sowers et al., 2023).