1. All OTUs (operational taxonomic units) raw sequences 2. Bacterial taxonomic structures at class and phylum level 3. Analytical data generated by multiple bioinformatics analyses. This dataset is not publicly accessible because: All the data were generated by THL in Finland. There is no EPA generated data. It can be accessed through the following means: Contact Dr. Tarja Pitkanen in Finnish Institute for Health and Welfare to request all the data used for the manuscript. Here is her contact information. Phone: +358 29 524 6315 Email: tarja.pitkanen@thl.fi. Format: Not available. This dataset is associated with the following publication: Tiwari, A., A. Hokajärvi, J. SantoDomingo, M. Elk, B. Jayaprakash, H. Ryu, S. Siponen, A. Vepsäläinen, A. Kauppinen, O. Puurunen, A. Artimo, N. Perkola, T. Huttula, I.T. Miettinen, and T. Pitkänen. Bacterial diversity and predicted enzymatic function in a multipurpose surface water system – from wastewater effluent discharges to drinking water production. Environmental Microbiome. BioMed Central Ltd, London, UK, 16(11): 17, (2021).

These data include detailed sample description (sampling locations, sampling events, DNA concentrations in four separate spreadsheet in an Excel file) and DNA sequencing plate layout. This dataset is associated with the following publication: Li, L., D. Ning, Y. Jeon, H. Ryu, J. SantoDomingo, D. Kang, A. Kadudula, and Y. Seo. Ecological Insights into Assembly Processes and Network Structures of Bacterial Biofilms in Full-scale Biologically Active Carbon Filters under Ozone Implementation. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 751: 141409, (2021).

These data include detailed sample description (sampling locations, sampling events, DNA concentrations in four separate spreadsheet in an Excel file) and DNA sequencing plate layout. This dataset is associated with the following publication: Li, L., D. Ning, Y. Jeon, H. Ryu, J. SantoDomingo, D. Kang, A. Kadudula, and Y. Seo. Ecological Insights into Assembly Processes and Network Structures of Bacterial Biofilms in Full-scale Biologically Active Carbon Filters under Ozone Implementation. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 751: 141409, (2021).

These data describe microbiological analyses performed on groundwater samples from domestic drinking water supply collected from 42 groundwater wells in the Central Valley of California. Samples were collected between January 2014 and April 2014 for the Groundwater Ambient Monitoring and Assessment (GAMA) program priority basin assessment of the Madera, Chowchilla, and Kings (MACK) groundwater sub-basins’ shallow aquifers. A total of 75 wells were sampled for the MACK study unit between August 2013 and April 2014. Samples for this dataset were vacuum filtered and plated on MI and mEI agars prior to incubation to promote colony growth. Colonies were tallied by their species into columns for various fecal indicator bacteria (FIBs): total coliforms (TCs), Escherichia coli (E. coli), enterococci. Non-target growths were also counted and tallied. Six additional replicate samples were collected for quality assurance. Of the 579 total FIB colonies detected, 106 were selected for polymerase chain reaction (PCR) analysis with the goal of sequencing their DNA. Selected colonies consisted of both target and non-target growths and were taken from 14 samples collected at 13 different wells. DNA sequencing was successful for 34 of the sampled colonies out of a total of 59 submitted. Results for these analyses were reported in FASTA format with the number of bases and their starting position indicated for each batch.

These data describe microbiological analyses performed on groundwater samples from domestic drinking water supply collected from 42 groundwater wells in the Central Valley of California. Samples were collected between January 2014 and April 2014 for the Groundwater Ambient Monitoring and Assessment (GAMA) program priority basin assessment of the Madera, Chowchilla, and Kings (MACK) groundwater sub-basins’ shallow aquifers. A total of 75 wells were sampled for the MACK study unit between August 2013 and April 2014. Samples for this dataset were vacuum filtered and plated on MI and mEI agars prior to incubation to promote colony growth. Colonies were tallied by their species into columns for various fecal indicator bacteria (FIBs): total coliforms (TCs), Escherichia coli (E. coli), enterococci. Non-target growths were also counted and tallied. Six additional replicate samples were collected for quality assurance. Of the 579 total FIB colonies detected, 106 were selected for polymerase chain reaction (PCR) analysis with the goal of sequencing their DNA. Selected colonies consisted of both target and non-target growths and were taken from 14 samples collected at 13 different wells. DNA sequencing was successful for 34 of the sampled colonies out of a total of 59 submitted. Results for these analyses were reported in FASTA format with the number of bases and their starting position indicated for each batch.

The bacteria sequence data generated in this study is available in the Short Read Archive (SRA) of NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA509718.

The bacteria sequence data generated in this study is available in the Short Read Archive (SRA) of NCBI (https://www.ncbi.nlm.nih.gov/) under BioProject PRJNA509718.

PS_GenBank.fasta file contain the sequences of the bacterial 16S rRNA-encoding gene for each representative sequence. The sequence containing four hypervariable regions was amplified using the primer set Eub-8f and 787. PS_GenBank.xlsx file contains rows as sample and columns as entries representing the accession number (NCBI) deposited in GenBank for each representative sequence (i.e. unique sequences). PS_OTU.fasta file contain the sequences of the bacterial 16S rRNA-encoding gene for each Operational Taxonomic Unit (OTU). The sequence containing four hypervariable regions was amplified using the primer set Eub-8f and 787. This dataset is associated with the following publication: Revetta , R., V. Gomez-Alvarez, T. Gerke, J. Santodomingo , and N. Ashbolt. CHANGES IN BACTERIAL COMPOSITION OF BIOFILM IN A METROPOLITAN DRINKING WATER DISTRIBUTION SYSTEM. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(1): 294-305, (2016).

PS_GenBank.fasta file contain the sequences of the bacterial 16S rRNA-encoding gene for each representative sequence. The sequence containing four hypervariable regions was amplified using the primer set Eub-8f and 787. PS_GenBank.xlsx file contains rows as sample and columns as entries representing the accession number (NCBI) deposited in GenBank for each representative sequence (i.e. unique sequences). PS_OTU.fasta file contain the sequences of the bacterial 16S rRNA-encoding gene for each Operational Taxonomic Unit (OTU). The sequence containing four hypervariable regions was amplified using the primer set Eub-8f and 787. This dataset is associated with the following publication: Revetta , R., V. Gomez-Alvarez, T. Gerke, J. Santodomingo , and N. Ashbolt. CHANGES IN BACTERIAL COMPOSITION OF BIOFILM IN A METROPOLITAN DRINKING WATER DISTRIBUTION SYSTEM. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(1): 294-305, (2016).