This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources. To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions. Starting in 2010, the regular La Parguera survey area was extended eastward to encompass the Guanica Bay region. The purpose of this modification was to conduct a baseline assessment of fish, macro-invertebrates (conch, lobster, Diadema) and benthic communities in support of Guanica Bay watershed restoration, and then to monitor changes over time. A watershed management plan was developed in 2008 by the Center for Watershed Protection, in cooperation with various Divisions of Puerto Rico DNER and NOAA, to identify priority management recommendations and implementation strategies for the Guanica watershed based on input from local experts, observations from on-the-ground assessments, and a comprehensive review of

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources.To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrate (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources.To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, reef fish assessment surveys were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) Cruise HA1101 in the Marianas region by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, 10 REA sites were surveyed at Guguan in the Marianas region. At each REA site, fish biologists entered the water and conducted a fine-scale (~700 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species diversity, size distribution, and abundance of fish in shallow-water hard-bottom (less than 30 m) habitats. Reef fish assessment surveys were focused on cataloging the diversity (species richness), abundance (numeric density) and biomass (fish mass per unit area) of diurnally active reef fish assemblages. The stationary point count (SPC) method was used to quantify reef fish species. Two divers lay out a 30 m transect line, and position themselves at the 7.5 and 22.5 meter marks. The SPC biologist then records estimated size and abundance of all fish within a visually estimated 15-m diameter cylinder centered on the stationary diver (7.5-m radius, total area ~ 177m^2 per cylinder). The diver first spends 5 minutes identifying all fish species in the cylindrical area, then proceeds to count and estimate size (total length) for each in a series of "instantaneous" point counts or sweeps of the cylinder. Fish were identified at the species level, wherever possible. All reef-associated fish, including those in the water column, were surveyed. The survey time for each stationary point count survey was approximately 20 min and generally four stationary point count surveys (two per diver) were conducted at each fish REA site. After completing REA surveys, divers noted the presence, at the survey site, of any unusual fish species not counted during SPC counts, in order to facilitate species lists per location.

This data set is derived from complementary projects operated in western and southwestern Puerto Rico. The research was designed 1) to compare the prevalence and impact of coral diseases on reefs across a gradient of human affects from mainland Puerto Rico, to more remote shelf edge reefs, and also uninhabited offshore islands (Mona Island and Desecheo Island) to understand factors that enhance or decrease disease impacts and document the resulting effects on reef fish assemblages, and 2) to monitor restoration success at the site of the M/V Fortuna Reefer grounding on Mona Island using Acropora palmata fragment reattachment, growth, and survival, incidence of coral disease, and composition of reef fish assemblages as indicators of recovery and reef health. The data consist of transect surveys (30 x 2 m) and stationary point counts (5m radius) of reef fish assemblages taken around permanent sites: identification to the lowest taxon possible (generally, genus and species), abundance, size estimates (fork length to the nearest cm). Samples are identified by location, date, time, and observer. Benthic data are maintained separately by co-PI (A. Bruckner).

These water quality data are one of many studies being done to assess and monitor coral reef ecosystems. The intent of this work is three fold: (1) to spatially characterize and monitor the distribution, abundance, and size of both reef fishes and mega-invertebrates (conch, lobster, Diadema); (2) to relate this information to in-situ data collected on water quality and associated habitat parameters; (3) to use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting and to establish the efficacy of those management decisions. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has completed its fourth year and is beginning its fifth year of work in the US Virgin Islands and Puerto Rico. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources.To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps ( less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and associated water quality and habitat information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

This fish and benthic composition database is the result of a multifaceted effort described below. The intent of this work is five fold: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting; 4) To establish the efficacy of those management decisions; and 5) To work with the National Coral Reef Monitoring Program to develop data collection standards and easily implemented methodologies for transference to other agencies and to work toward standardizing data collection throughout the US states and territories. Toward this end, the Center for Coastal Monitoring and Assessment's Biogeography Branch (BB) has been conducting research in Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. It is critical, with recent changes in management at both locations (e.g. implementation of MPAs) as well as proposed changes (e.g. zoning to manage multiple human uses) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas. It is also important that BB work closely with the individuals responsible for recommending and implementing these management strategies. Recognizing this, BB has been collaborating with partners at the University of Puerto Rico, National Park Service, US Geological Survey and the Virgin Islands Department of Planning and Natural Resources. To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work therefore, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are stratified to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef or seagrass area. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, BB is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between locations. Armed with the knowledge of where "hot spots" of species richness and diversity are likely to occur in the seascape, the BB is in a unique position to answer questions about the efficacy of marine zoning strategies (e.g. placement of no fishing, anchoring, or snorkeling locations), and what locations are most suitable for establishing MPAs. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

This fish and benthic composition database is the result of a multifaceted effort described below.The National Oceanic and Atmospheric Administration's (NOAA) Biogeography Branch, in consultation with NOAA's Office of Response and Restoration (ORandR) and other local and regional experts, is conducting an ecological characterization of the marine ecosystem around Vieques Island, Puerto Rico. The assessment will support effective management and conservation of marine resources in Vieques as a whole. To date a spatially comprehensive assessment of coral reef and hardbottom habitat around Vieques has been lacking. To fill this gap, the Biogeography Branch is expanding long term monitoring efforts to Vieques to collect detailed information about the benthic habitats, fish, and invertebrate communities. Spatially comprehensive information on reefs and hardbottom is vital to future management of the marine resources around Vieques. The collected data will be used to quantify the abundance and spatial distribution of fish, corals, and benthic invertebrates on hardbottom habitats around Vieques. Further, with regular monitoring, changes in the composition and condition of Vieques reefs over time can be detected.Data is collected using established protocols and monitoring efforts consistent with NOAA's National Coral Reef Monitoring Program and the Biogeography Branch's long-term monitoring efforts in southwest Puerto Rico and the US Virgin Islands since 2000 and 2001, respectively. The intent of this work is: 1) To spatially characterize and monitor the distribution, abundance, and size of both reef fishes and macro-invertebrates (conch, lobster, Diadema); 2) To relate this information to in-situ data collected on associated benthic composition parameters; and 3) To use this information to establish the knowledge base necessary for enacting management decisions in a spatial setting. It is critical, with recent changes in land and maritime use in Vieques (i.e., transfer of former Navy lands to the Fish and Wildlife Service, the Municipality in Vieques, and the Puerto Rico Conservation Trust) that action is taken now to accurately describe and characterize the fish/macro-invertebrate populations in these areas.To quantify patterns of spatial distribution and make meaningful interpretations, we must first have knowledge of the underlying variables determining species distribution. The basis for this work, is the nearshore benthic habitats maps (less than 100 ft depth) created by NOAA's Biogeography Program in 2001 and NOS' bathymetry models. Using ArcView GIS software, the digitized habitat maps are used to select reef/hardbottom habitat that is further stratified by proximity to former land use and geographic side of the island (i.e., north vs. south) to select sampling stations. Sites are randomly selected within these strata to ensure coverage of the entire study region and not just a particular reef. At each site, fish, macro-invertebrates, and benthic composition information is then quantified following standardized protocols. By relating the data collected in the field back to the habitat maps and bathymetric models, the Biogeography Branch is able to model and map species level and community level information. These protocols are standardized throughout the US Caribbean to enable quantification and comparison of reef fish abundance and distribution trends between Vieques and the other locations. Knowledge of the current status of fish/macro-invertebrate communities coupled with longer term monitoring will enable evaluation of management efficacy, thus it is essential to future management actions.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 15 April - 7 May 2009, reef fish assessment surveys were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) Cruise HI0903 in the Marianas Archipelago at biennial intervals by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, 6 REA sites were surveyed at Guguan Island in the Marianas Archipelago. At the specific REA sites, fish biologists along with coral biologists, algal biologists and marine invertebrate zoologist entered the water and conducted a fine-scale (~300 m2) and high degree of taxonomic resolution REA survey to assess and monitor species composition, abundance, percent cover, size distribution, diversity, and general health of fish, corals, macro-invertebrates, and algae in shallow-water (< 35 m) habitats. Reef fish assessment surveys were focused on cataloging the diversity (species richness), abundance (numeric density [# fish 100 m-2] and biomass density [kg 100 m-2]) of diurnally active reef fish assemblages. Three complementary noninvasive underwater survey methods were used, including belt-transect, stationary point count, and roving-diver surveys. For all methods, fish were identified at the species level, when possible, and assigned to a size bin ranging from 1 to 200 cm based on a visual estimate of total fish length. Belt-transect (BLT) surveys were used to quantify the entire diurnal fish community (all size classes). In belt-transect surveys, two fish biologists swam side-by-side along three consecutively-placed, 25m transect lines. The BLT team swam each transect two times. During the initial swim-out, each fish diver recorded all fish larger than 20 cm observed within a 4-m wide belt perpendicular to their respective side of the transect (200 m2 area per line, 100 m2 per diver). On the return swim, each fish diver recorded all fish less than 20 cm observed within a 2-m wide belt (100 m2 area per line, 50 m2 per diver). The survey of large fish took approximately 5 min to complete while the survey of smaller fish took about 10 min to complete. All reef-associated fish, including those in the water column (including planktivores), were counted. Any coastal pelagic species (e.g., clupeids [sardines], belonids [beakfish], antherinids [silversides]) seen near the surface were not recorded. The stationary point count (SPC) method were used to quantify larger, more mobile reef fish species that can be missed on belt-transect surveys. In stationary point count survey, a fish biologist swam approximately 15 m away from a transect line concurrently being surveyed by the other two BLT fish biologists. The SPC biologist then recorded all fish greater than 25 cm in length that passed within a visually estimated 20-m diameter cylinder centered on the diver's fixed position (10-m radius, total area = 314 m2). The survey time for each stationary point count survey was 5 min and a total of four stationary point count surveys were conducted at each REA site. Roving-diver surveys were followed belt-transect and stationary point count surveys. As diver bottom time permitted, the fish assessment team conducted random swim surveys throughout the REA site area, recording, to the species level or the lowest recognizable taxon, the presence of reef fish not encountered during previous methods.

To support a long-term NOAA Coral Reef Conservation Program (CRCP) for sustainable management and conservation of coral reef ecosystems, from 20110407 to 20110509, reef fish assessment surveys were conducted, as a part of Rapid Ecological Assessments (REA), during the Pacific Reef Assessment and Monitoring Program (RAMP) Cruise HA1101 in the Marianas region by the Coral Reef Ecosystem Division (CRED) at the NOAA Pacific Islands Fisheries Science Center (PIFSC). During the cruise, 52 REA sites were surveyed at Guam in the Marianas region. At each REA site, fish biologists entered the water and conducted a fine-scale (~700 m^2) and high degree of taxonomic resolution REA survey to assess and monitor species diversity, size distribution, and abundance of fish in shallow-water hard-bottom (less than 30 m) habitats. Reef fish assessment surveys were focused on cataloging the diversity (species richness), abundance (numeric density) and biomass (fish mass per unit area) of diurnally active reef fish assemblages. The stationary point count (SPC) method was used to quantify reef fish species. Two divers lay out a 30 m transect line, and position themselves at the 7.5 and 22.5 meter marks. The SPC biologist then records estimated size and abundance of all fish within a visually estimated 15-m diameter cylinder centered on the stationary diver (7.5-m radius, total area ~ 177m^2 per cylinder). The diver first spends 5 minutes identifying all fish species in the cylindrical area, then proceeds to count and estimate size (total length) for each in a series of "instantaneous" point counts or sweeps of the cylinder. Fish were identified at the species level, wherever possible. All reef-associated fish, including those in the water column, were surveyed. The survey time for each stationary point count survey was approximately 20 min and generally two stationary point count surveys (one per diver) were conducted at each fish REA site. After completing REA surveys, divers noted the presence, at the survey site, of any unusual fish species not counted during SPC counts, in order to facilitate species lists per location.