Data describe the results of a controlled laboratory mesocosm experiment evaluating the influence of a nitrifier enriched microbial community on silver carp (Hypophthalmichthys molitrix) milt eDNA degradation. Parameters described include the concentration results, limit of detection, and limit of quantification of two silver carp specific quantitative PCR assays and water chemistry results of experimental mesocosms.

These data describe the results of a controlled laboratory mesocosm experiment evaluating the effect of nitrapyrin, a nitrification inhibitor, on nitrification in a nitrifier enriched microbial (NEM) community. Parameters described include the water chemistry and nitrapyrin measurement results of experimental mesocosms. Microbial community data is described in a data release linked to this one.

These data were collected to determine the effect of nitrapyrin, a commercial nitrification inhibitor, on nitrification by a diverse nitrifier-enriched microbial (NEM) community. The NEM community was originally collected from an aquaculture pond in Columbia, Missouri and cultured in HEPES-buffered, minimal mineral salts media, supplemented with a nominal dose of 2 milligrams per liter ammonia-nitrogen, added as ammonium-sulfate. NEM were exposed to nitrapyrin (0 to 32 micrograms per liter) for 192 hours. Before dosing, the nitrapyrin was dissolved in dimethyl sulfoxide (DMSO). After dosing, the DMSO concentration in the flasks was 0.1 percent volume to volume. Ammonia, nitrite, nitrate, pH, dissolved oxygen, temperature, water hardness, conductivity, alkalinity, turbidity, and nitrapyrin were monitored at intervals during the experiment.

Wetlands are frequently used in the U.S. for treating nitrate in agricultural runoff. However, numerous other biologically active contaminants, such as antibiotics and nitrification inhibitors, regularly co-occur with nitrate and many can affect the efficiency of nitrate removal. This project evaluated the discrete and combined effects of specific veterinary and human antibiotics and a common nitrification inhibitor (nitrapyrin) on nitrate-N treatment efficiency in saturated sediments and floating treatment wetlands. Sediment and water samples were collected from 3 locations at the USDA Meat Animal Research Facility near Clay Center NE in August 2019 for assessment of background conditions in order to determine the ideal collection site for future microcosm experiments to quantify rates of N-cycling activity in sediments. The sites ranged in historical exposure to inhibitors from (1) little to no relative historical exposure (Control site) (2) moderate relative historical exposure (Grade Control Structure 5), and (3) heavy relative historical exposure to antibiotic runoff from agricultural and cattle grazing land (Reuse Pit (RP)). Laboratory microcosm experiments were then initiated in December 2019 using sediment collected from the historically high exposure site (Reuse Pit) only and an artificial water prepared based on chemistry of the water measured in August 2019. One set of microcosms was amended with an antibiotic mixture (chlortetracycline, sulfadimethoxine, lincomycin, monensin) and another set with nitrapyrin. The targeted range of concentrations for both sets was 0 to 1000 microgram per L. Sediments were exposed to the inhibitors for 3 weeks prior to initiation of the experiments to ensure the microbial communities were affected by the presence of the inhibitors. Anaerobic denitrification and aerobic nitrification potential rates were then quantified after the 3-week exposure period, and samples were collected for microbial community structure and gene abundance. Background sediments from August and December 2019 collections were characterized for potassium chloride extractable nitrogen species, total percent carbon and nitrogen content, microbial community composition, and gene abundance. And background water chemistry was measured from both collections. Data from the N-cycling measurements, molecular analyses, inhibitor concentrations, and background sediment and water characterization are documented in this data release.

SOUR (oxygen uptake rates) and qPCR for nitrification genes. This dataset is associated with the following publication: Kapoor , V., X. Li , M. Elk , K. Chandran, C. Impellitteri , and J. Santodomingo. Transcriptional and physiological responses of nitrifying bacteria to heavy metal inhibition. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 49: 13454-13462, (2015).

SOUR and qPCR data. This dataset is associated with the following publication: Kapoor, V., M. Elk, and X. Li. Inhibitory effect of cyanide on wastewater nitrification determined using SOUR and RNA-based gene-specific assays. Letters in Applied Microbiology. Blackwell Publishing, Malden, MA, USA, 63(2): 155-161, (2016).

SOUR and qPCR data. This dataset is associated with the following publication: Kapoor, V., M. Elk, and X. Li. Inhibitory effect of cyanide on wastewater nitrification determined using SOUR and RNA-based gene-specific assays. Letters in Applied Microbiology. Blackwell Publishing, Malden, MA, USA, 63(2): 155-161, (2016).

This data package was created 2025-12-03 08:20:34 by NPSTORET and includes selected project, location, and result data. Data are from Ernest A. Matson's (University of Guam's Marine Laboratory) 1990 Technical Report (#93) entitled "Effects of the Agat, Agana, and Northern District Wastewater Effluents on Receiving Water Quality (Chemistry and Bacteriology) and the Effects of the Agat Effluent on Success of Sea Urchin Egg Fertilization". Data contained in WAPA_NPSTORET_BE.ACCDB (NPSTORET back-end file) were filtered to include: Organization: - WAPA: War in the Pacific National Historical Park Project: - WAPA0009: Effects of the Agat and Agana Wastewater Effluents on Receiving Water Quality Station: - Include Trip QC And All Station Visit Results Value Status: - Accepted or Certified (exported as Final) or Final The data package is organized into five data tables: - Projects.csv - describes the purpose and background of the monitoring efforts - Locations.csv - documents the attributes of the monitoring locations/stations - Results.csv - contains the field measurements, observations, and/or lab analyses for each sample/event/data grouping - HUC.csv - enumerates the domain of allowed values for 8-digit and 12-digit hydrologic unit codes utilized by the Locations data table - Characteristics.csv - enumerates the domain of characteristics available in NPSTORET to identify what was sampled, measured or observed in Results Period of record for filtered data is 1989-02-09 to 1989-12-12. This data package is a snapshot in time of one National Park Service project. The most current data for this project, which may be more or less extensive than that in this data package, can be found on the Water Quality Portal at: https://www.waterqualitydata.us/data/Result/search?project=WAPA0009&mimeType=csv&zip=yes&dataProfile=biological&providers=STORET

These data have been collected as part of a cooperative project between the U.S. Geological Survey (USGS) and the U.S Environmental Protection Agency. This project began in 2016 and consisted of a mesocosm experiment quantifying short term responses by a transplanted natural diatom dominant algae community exposed to gradients of nitrogen, phosphorous, and a mixture of both nitrogen and phosphorus. The data consist of water quality parameters such as temperature and pH as well as aqueous nutrient concentrations and algae responses, including chlorophyll a concentrations, algae dry mass, algae ash-free dry mass, and intra-cellular phosphorus and nitrogen in algae. Algae growth rates were estimated from these data and are meant to be used with water-quality models; the results of this investigation can be found in the manuscript associated with this data release (Schmidt and others 2019).

This dataset includes laboratory data collected to determine the effects of formaldehyde on nitrification processes in biofilters of recirculating aquaculture systems (RAS). Data from the laboratory studies were used to inform methods used to conduct field trials in a pilot and commercial-scale RAS. Biofilter nitrification was monitored by measuring the levels of total ammonia nitrogen, nitrite, and nitrate in experimental aquaria containing a small biofilter before and after formaldehyde was applied to the test system.