We determined acute and chronic toxicity of sodium chloride (NaCl) and sodium sulfate (Na2SO4) to up to 7 freshwater species of different taxa in a hardness water of 40 mg/L as CaCO3 and evaluated the effect of 4 hardness waters (15, 30, 60, and 120 mg/L as CaCO3) on acute toxicity of NaCl and Na2SO4 to 3 sensitive species tested in the acute exposures. This data release reports results from acute and chronic exposures with Ceriodaphnia dubia (cerios), Chironomus dilutus (midge), Hyalella azteca (amphipod), Hyla versicolor (gray treefrog), Lampsilis abrupta (pink mucket), Lampsilis siliquoidea (fatmucket), and Pimephales promelas (fathead minnow. Date include survival, size, and other endpoints and associated water quality measurements.

The responses (survival, growth, and/or reproduction) of test organisms in six concentrations of toxicants in several test waters with different water quality characteristics. In addition to the individual biological data, chemical, and water quality measurements from each toxicity test are also reported. Test organisms include unionid mussels (Lampsilis siliquoidea, Villosa iris), a midge (Chironomus dilutus), fish (rainbow trout Oncorhynchus mykiss and fathead minnow, Pimephales promelas), 2 amphibians (Hyla versicolor, Lithobates sylvaticus), and an amphipod (Hyalella azteca).

The responses (survival, growth, and/or reproduction) of test organisms in six concentrations of toxicants in several test waters with different water quality characteristics. In addition to the individual biological data, chemical, and water quality measurements from each toxicity test are also reported. Test organisms include unionid mussels (Lampsilis siliquoidea, Villosa iris), a midge (Chironomus dilutus), fish (rainbow trout Oncorhynchus mykiss and fathead minnow, Pimephales promelas), 2 amphibians (Hyla versicolor, Lithobates sylvaticus), and an amphipod (Hyalella azteca).

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

The data consists of the responses (survival, growth, and/or reproduction) of test organisms were determined in six concentrations of toxicants in 7-day toxicity tests or in four different feeding rates in 7-day feeding experiments. Specifically we evaluated the sensitivity of 2 mussel species (Villosa constricta and Lampsilis siliquoidea) and P. promelas and C. dubia using effluents in 7-d exposures. We then refined the method by determining the best feeding rate of algal mixture for 1-, 2-, and 3-wk-old L. siliquoidea in a 7-d feeding experiment, and using derived optimal feeding rates to assess the sensitivity of the 3 ages of juveniles in a 7-d NaCl test. Finally, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-d NaCl test with L. siliquoidea.

Attributes regarding the acute toxicity of sodium chloride (NaCl) and potassium chloride (KCl) to larvae (glochidia) or juveniles of a unionid mussel (fatmucket, Lampsilis siliquoidea) at various water hardness and in the presence of other major ions.

Attributes regarding the acute toxicity of sodium chloride (NaCl) and potassium chloride (KCl) to larvae (glochidia) or juveniles of a unionid mussel (fatmucket, Lampsilis siliquoidea) at various water hardness and in the presence of other major ions.

This dataset provides data files and software regarding the report "EVALUATIONS IN SUPPORT OF THE DEVELOPMENT OF AQUATIC LIFE CRITERIA FOR MAJOR GEOCHEMICAL IONS: TOTAL ION TOXICITY VERSUS CALCIUM FOR SEVERAL AQUATIC SPECIES". This includes eight excel files containing data on multiple tests (of different salts, salt mixtures, and test conditions) regarding the acute toxicity of major geochemical ions to eight aquatic species. These data provide the chemistry of test solutions at median lethal conditions in these tests and were used in subsequent analyses described in the report to derive the relationships for total ion toxicity versus calcium concentration for these eight species (for subsequent use in criteria development). A Word file describing these files is provided, and additional files regarding the analyses are archived and subject to request. The dataset also provides two zip files containing software used in these analyses, including software for determining concentration-response curves (to quality assure author-reported LC50s during report preparation) and the software for establishing the total ion toxicity vs calcium relationships. Each zip file contains an executable file and the Fortran code used to build these executables for code review as desired, but are not recompilable into a new executables without Winteracter (a Fortran-based user interface development application). This dataset is associated with the following publication: Erickson, R. Evaluations in support of the development of aquatic life criteria for major geochemical ions: Total ion toxicity versus calcium for several aquatic species. U.S. Environmental Protection Agency, Washington, DC, USA, 2025.

Biological endpoints for three focal test species [Daphnia magna (mortality), Psuedacris maculata (mortality, growth, and development), and Lemna gibba (growth)] in response to exposure to reconstituted or field-collected water under laboratory conditions. Field-collected waters were collected from wetlands within the Prairie Pothole Region (PPR) of the United States. Reconstituted water was mixed to mimic some chemistry of surface water from brine contaminated PPR wetlands. Also included are water quality and chemical concentration data from all assays.

Biological endpoints for three focal test species [Daphnia magna (mortality), Psuedacris maculata (mortality, growth, and development), and Lemna gibba (growth)] in response to exposure to reconstituted or field-collected water under laboratory conditions. Field-collected waters were collected from wetlands within the Prairie Pothole Region (PPR) of the United States. Reconstituted water was mixed to mimic some chemistry of surface water from brine contaminated PPR wetlands. Also included are water quality and chemical concentration data from all assays.