The data include temperature and relative humidity for the positive controls; T, RH, and hydrogen peroxide concentrations that the test coupons were exposed to in each experiment; and then the plaque forming unit data. The PFU data for each experiment are in separate Excel files. Each file with the PFU data shows the PFU recovered for every material, timepoint, and replicate; for both positive controls and test coupons. The log reduction calculations are also included in each file. This dataset is associated with the following publication: Wood, J., W. Richter, M. Sunderman, M. Calfee, S. Serre, and R. Mickelsen. Evaluating the Environmental Persistence and Inactivation of MS2 Bacteriophage and the Presumed Ebola Virus Surrogate Phi6 Using Low Concentration Hydrogen Peroxide Vapor. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 10, (2020).

Dataset includes data from each experiment conducted in the pilot-scale testing. Each sheet of the Excel file pertains to each test. A data dictionary is included in the first sheet. In each sheet there are microbiological data (colony forming units) for each test and positive control coupon used in the study. Also shown is the calculation of decontamination efficacy (log10 reduction). This dataset is associated with the following publication: Wood, J., W. Calfee, S. Ryan, L. Mickelsen, M. Clayton, and V. Rastogi. A Simple Decontamination Approach Using Hydrogen Peroxide Vapor for Bacillus anthracis Spore Inactivation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(6): 1603-1615, (2016).

Dataset includes data from each experiment conducted in the pilot-scale testing. Each sheet of the Excel file pertains to each test. A data dictionary is included in the first sheet. In each sheet there are microbiological data (colony forming units) for each test and positive control coupon used in the study. Also shown is the calculation of decontamination efficacy (log10 reduction). This dataset is associated with the following publication: Wood, J., W. Calfee, S. Ryan, L. Mickelsen, M. Clayton, and V. Rastogi. A Simple Decontamination Approach Using Hydrogen Peroxide Vapor for Bacillus anthracis Spore Inactivation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(6): 1603-1615, (2016).

Data from small lab scale tests conducted at ECBC. It contains efficacy data as well as data on env conditions such as temperature, RH, and hydrogen peroxide vapor concentration. This dataset is associated with the following publication: Wood, J., W. Calfee, S. Ryan, L. Mickelsen, M. Clayton, and V. Rastogi. A Simple Decontamination Approach Using Hydrogen Peroxide Vapor for Bacillus anthracis Spore Inactivation. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 121(6): 1603-1615, (2016).

The data set is comprised of five Excel spreadsheets, one for each of the tests described in the research article. The data in the spreadsheets are the colony forming unit (CFU) data for each coupon material replicate and location. This dataset is associated with the following publication: Mickelsen, L., J. Wood, W. Calfee, S. Serre, S. Ryan, A. Touati, F. Delafield, and D. Aslett. Low‐concentration hydrogen peroxide decontamination for Bacillus spore contamination in buildings. Remediation Journal. John Wiley & Sons, Inc., Hoboken, NJ, USA, 30(1): 47-56, (2019).

In the 2014 West African Ebola outbreak, international organizations provided conflicting recommendations for disinfecting surfaces contaminated by uncontrolled patient spills. We compared the efficacy of four chlorine solutions (sodium hypochlorite, sodium dichloroisocyanurate, high test hypochlorite, and generated hypochlorite) for disinfection of three surface types (stainless steel, heavy duty tarp, and nitrile), with and without precleaning practices (prewiping and/or covering) and soil load. The test organisms were E. coli and the Ebola surrogate Phi6. All tests achieved a minimum of 5.9 and 3.1log removal in E. coli and Phi6, respectively. A 15-minute exposure to 0.5% chlorine was sufficient to ensure less than 8 Phi6 PFU/cm2 in all tests. While chlorine types were equally efficacious with and without soil load, variation was seen by surface type. Wiping did not increase disinfection efficacy, and is not recommended as it generates infectious waste. Covering spills decreased disinfection efficacy against E. coli on heavy duty tarp but does prevent splashing, which is critical in Ebola contexts. Our results support the recommendation of a 15-minute exposure to 0.5% chlorine, independently of chlorine type, surface, pre-cleaning practices, and organic matter, as an efficacious measure to interrupt disease transmission from uncontrolled spills in Ebola outbreaks.

In the 2014 West African Ebola outbreak, international organizations provided conflicting recommendations for disinfecting surfaces contaminated by uncontrolled patient spills. We compared the efficacy of four chlorine solutions (sodium hypochlorite, sodium dichloroisocyanurate, high test hypochlorite, and generated hypochlorite) for disinfection of three surface types (stainless steel, heavy duty tarp, and nitrile), with and without precleaning practices (prewiping and/or covering) and soil load. The test organisms were E. coli and the Ebola surrogate Phi6. All tests achieved a minimum of 5.9 and 3.1log removal in E. coli and Phi6, respectively. A 15-minute exposure to 0.5% chlorine was sufficient to ensure less than 8 Phi6 PFU/cm2 in all tests. While chlorine types were equally efficacious with and without soil load, variation was seen by surface type. Wiping did not increase disinfection efficacy, and is not recommended as it generates infectious waste. Covering spills decreased disinfection efficacy against E. coli on heavy duty tarp but does prevent splashing, which is critical in Ebola contexts. Our results support the recommendation of a 15-minute exposure to 0.5% chlorine, independently of chlorine type, surface, pre-cleaning practices, and organic matter, as an efficacious measure to interrupt disease transmission from uncontrolled spills in Ebola outbreaks.

Dataset contains efficacy data, as well as temperature and RH data. The data describe inactivation of non-spore-forming biological agents on surfaces following exposure to different temperature and humidity environments. This dataset is associated with the following publication: Richter, W., M. Sunderman, M. Wendling, S. Serre, L. Mickelsen, R. Rupert, J. Wood, Y. Choi, Z. Willenberg, and M. Calfee. Evaluation of Altered Environmental Conditions as a Decontamination Approach for Non-Spore-Forming Biological Agents-Journal Article. JOURNAL OF APPLIED MICROBIOLOGY. Blackwell Publishing, Malden, MA, USA, 128(4): 1050-1059, (2019).

The SARS-CoV-2 virus has put a strain on the supply and distribution of N95 masks, with some hospitals electing to reuse masks after disinfection. Currently, vaporized hydrogen peroxide (VHP) mask disinfection systems are being deployed at multiple locations throughout the United States, for example in shipping containers and in small rooms. Such enclosures will be of a different size, have different surface materials and have varying air change rates; all these parameters will impact the VHP dose that the masks receive. This spreadsheet tool can be used to estimate the VHP concentration in air of such a room. The tool employs a single-zone mass balance analysis that accounts for room size, VHP losses to surfaces and air change rate. This tool is intended to support VHP disinfection efforts by providing estimates of VHP concentrations in the room being employed, but it does not describe or provide guidance on VHP disinfection applications.

The SARS-CoV-2 virus has put a strain on the supply and distribution of N95 masks, with some hospitals electing to reuse masks after disinfection. Currently, vaporized hydrogen peroxide (VHP) mask disinfection systems are being deployed at multiple locations throughout the United States, for example in shipping containers and in small rooms. Such enclosures will be of a different size, have different surface materials and have varying air change rates; all these parameters will impact the VHP dose that the masks receive. This spreadsheet tool can be used to estimate the VHP concentration in air of such a room. The tool employs a single-zone mass balance analysis that accounts for room size, VHP losses to surfaces and air change rate. This tool is intended to support VHP disinfection efforts by providing estimates of VHP concentrations in the room being employed, but it does not describe or provide guidance on VHP disinfection applications.