This data release contains dissolved cyanotoxin concentrations for microcystins (MC), cylindrospermopsins (CYL), anatoxins (ATX), and saxitoxins (STX) assessed using enzyme-linked immunosorbent assays (ELISA) and liquid chromatography-mass spectrometry or tandem mass spectrometry (LC-MS and LC-MS/MS, respectively) in extracts from SPATT (n=95) and DGT samplers (n=10) deployed on three Finger Lakes (Owasco, Seneca, and Skaneateles) between June and November 2019. Relative percent contributions of microcystin congeners are also provided for samples analyzed by LC-MS and LC-MS/MS. Solid phase adsorption toxin tracking (SPATT) and diffusive gradients in thin-films (DGT) are types of passive environmental samplers containing porous synthetic resin beads that adsorb and concentrate cyanotoxins over time. SPATT and DGT samplers are relatively inexpensive to construct and have been widely demonstrated to adsorb a range of cyanotoxins in different environments. These samplers can be deployed in water bodies for time periods ranging from hours to weeks and, upon extraction and analysis, provide a semi-quantitative, relative dissolved cyanotoxin concentration that is calculated based on the deployment length.

This data release presents cyanotoxin and multiparameter water-quality sonde data collected from July-October 2020, at eleven sites in the Salem River, New Jersey. Data-collection sites included seven flowing and four impounded reaches of the main stem Salem River. Solid Phase Adsorption Toxin Tracking (SPATT) samplers were deployed at 11 sampling locations during the study period and retrieved after 1-15 days. Discrete grab samples also were collected at each sampling location during SPATT deployment and retrieval. Physicochemical measurements were collected in-situ with a multiparameter water-quality sonde when the SPATT samplers were deployed and retrieved and include measurements of water temperature, pH, specific conductance, dissolved oxygen concentration, and turbidity. Because of analytical expenses, discrete cyanotoxin samples were analyzed at only 10 of the 11 discrete sampling locations, and toxin results from these analyses informed selection of only 4 of the 11 SPATT sampling locations for additional analyses. SPATT sampler extracts and discrete-grab samples were analyzed for four cyanotoxins (anatoxin-a, cylindrospermopsin, microcystin, saxitoxin) by enzyme-linked immunosorbent assay (ELISA).

In cooperation with the Tennessee Wildlife Resources Agency (TWRA) and University of Tennessee Knoxville (UTK), the U.S. Geological survey (USGS) collected cyanobacterial toxin concentrations (i.e., microcystin, anatoxin, cylindrospermopsin, and saxitoxin) and water-quality field data at 4 sites on the Clinch River from November 2020 to December 2023. The goal for the sample data was to better understand the occurrence and distribution of cyanobacterial toxins in a section of the Clinch River. Sample collection was primarily during the growing season, when harmful algal blooms (HABs) are known to be most active. This data release documents the toxin concentrations and water-quality data produced from the study.

In cooperation with the Tennessee Department of Environment and Conservation (TDEC), the U.S. Geological Survey (USGS) measured cyanobacterial toxin concentrations (i.e., microcystin, anatoxin, cylindrospermopsin, and saxitoxin) and collected water-quality field data at 18 sites in North-central Tennessee from August 2022 to November 2024. Additionally, selected sites also were sampled once for environmental DNA (eDNA) analysis to confirm the presence of toxin producing DNA at the monitoring sites. The goal for the sample data was to better understand the occurrence and distribution of cyanobacterial toxins in various freshwater reservoir types in North-central Tennessee. Sample collection was primarily during the growing season, when harmful algal blooms (HABs) are known to be most active. This data release documents the sites, toxin concentrations, water-quality data, and eDNA data produced from the study.

This data release contains cyanotoxin concentrations for microcystins, cylindrospermopsins, anatoxins, and saxitoxins assessed using Enzyme-Linked Immunosorbent Assays (ELISA) on 363 samples collected from 82 surface-water sites located in the Cascade Range in Oregon, and eight sites located outside of the Oregon Cascade Range in Washington and California, during 2016-2020. Three sample types were assessed: 1) benthic colonies and mats of cyanobacteria (n=109), 2) plankton net tows (n=90), and 3) Solid Phase Adsorption Toxin Tracking samplers (SPATTs)(n=164).

This data release contains cyanotoxin concentrations for microcystins, cylindrospermopsins, anatoxins, and saxitoxins assessed using Enzyme-Linked Immunosorbent Assays (ELISA) on 363 samples collected from 82 surface-water sites located in the Cascade Range in Oregon, and eight sites located outside of the Oregon Cascade Range in Washington and California, during 2016-2020. Three sample types were assessed: 1) benthic colonies and mats of cyanobacteria (n=109), 2) plankton net tows (n=90), and 3) Solid Phase Adsorption Toxin Tracking samplers (SPATTs)(n=164).

This dataset is comprised of cyanotoxin concentrations in extracts from solid phase adsorption toxin tracking (SPATT) samplers deployed at fixed sampling stations in the Sacramento-San Joaquin Delta, beginning in 2020. SPATT samplers are a form of passive sampler constructed of adsorbent resin beads that concentrate cyanotoxins over time. Cyanotoxin congeners were measured from SPATT extracts within the cyanotoxin classes: microcystins, anatoxins, cylindrospermopsins, saxitoxins, anabaenopeptins, and nodularin. Concentrations were measured using liquid chromatography tandem mass spectrometry (LC-MS/MS), with some samples also measured with enzyme linked immunosorbent assay (ELISA).

The EPA National Coastal Condition Assessment (NCCA) is a nation-wide survey of coastal and estuarine water quality. During the 2015 EPA NCCA, samples were collected for analysis of algal toxins and cyanotoxins at the Organic Geochemistry Research Laboratory (OGRL) at the U.S. Geological Survey Kansas Water Science Center (KSWSC) by liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS). The 542 samples collected from the Great Lakes were analyzed for anatoxin-a, cylindrospermopsin, domoic acid, 10 microcystin congeners, nodularin, and okadaic acid. A subset of samples were also analyzed for dinophysistoxin-1, dinophysistoxin-2, gymnodimine, pectenotoxin-2, and 13-desmethyl spirolide c.

Cyanobacteria known to produce cyanotoxins have been reported worldwide. As monitoring efforts have improved, they have been detected in not only freshwater, but also in estuary and marine waters. To assess the occurrence of cyanotoxins and algal toxins in California estuaries, the NOAA MERHAB funded project collected samples monthly from 11 Californian estuary locations, with 5 locations sampled in 2016 as event response. This data release includes liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) results for 21 cyanotoxins and algal toxins in estuary samples collected from California in 2016-2017.

Cyanobacteria known to produce cyanotoxins have been reported worldwide. As monitoring efforts have improved, they have been detected in not only freshwater, but also in estuary and marine waters. To assess the occurrence of cyanotoxins and algal toxins in California estuaries, the NOAA MERHAB funded project collected samples monthly from 11 Californian estuary locations, with 5 locations sampled in 2016 as event response. This data release includes liquid chromatography triple quadrupole mass spectrometry (LC/MS/MS) results for 21 cyanotoxins and algal toxins in estuary samples collected from California in 2016-2017.